Enhancing peripheral nerve regeneration: A novel nanofibrous nerve conduit with bioactive poly(ɛ-caprolactone), collagen, and retinoic acid nanofiber
Objective(s): Peripheral nerve injury (PNI) is a critical clinical issue primarily caused by trauma. Tissue engineering approaches using nanofiber scaffolds have been extensively explored to improve material quality and create an environment resembling the natural extracellular matrix (ECM). Materia...
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Mashhad University of Medical Sciences
2025-01-01
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| Series: | Nanomedicine Journal |
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| Online Access: | https://nmj.mums.ac.ir/article_24903_f0e1a4ca975176489810fa793dcfa484.pdf |
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| author | Muhammad Aseer Niloofar Nazeri Nasrollah Tabatabaei Zohreh Arabpour Reza Faridi Majidi Hossein Ghanbari |
| author_facet | Muhammad Aseer Niloofar Nazeri Nasrollah Tabatabaei Zohreh Arabpour Reza Faridi Majidi Hossein Ghanbari |
| author_sort | Muhammad Aseer |
| collection | DOAJ |
| description | Objective(s): Peripheral nerve injury (PNI) is a critical clinical issue primarily caused by trauma. Tissue engineering approaches using nanofiber scaffolds have been extensively explored to improve material quality and create an environment resembling the natural extracellular matrix (ECM). Materials and Methods: In this study, we employed electrospinning technique to fabricate a composite scaffold comprising poly(ɛ-caprolactone) (PCL) and collagen (Col) loaded with all-trans retinoic acid (RA), a neural patterning and signaling chemical known to promote nerve regeneration. Results: The synthesized nanofiber scaffold exhibited a diameter of 391±79 nm and a tensile strength of 250±13 MPa, providing sufficient support for native peripheral nerve regeneration. The inclusion of Col enhanced the scaffold’s hydrophilic behavior (contact angle: 43±6°), ensuring stability in an aqueous solution. Moreover, the results demonstrated the proliferation and adhesion of nerve cells on the scaffold, aligning with the directions of the warp and weft of the nanofiber mat. Importantly, the scaffolds demonstrated non-toxicity, making them a promising substitute for the native ECM for enhanced cell attachment and proliferation. Finally, immune-histochemistry analyses further confirmed that the scaffolds supported the release and growth of neurites, promoting cell differentiation toward nerve repair. Conclusion: The RA-loaded scaffolds demonstrated the enhanced biocompatibility, supported neurite growth, and showed potential as a capable candidate for nerve regeneration. |
| format | Article |
| id | doaj-art-7d8cf06c526e4d7fbd23b7788a022bb7 |
| institution | Kabale University |
| issn | 2322-3049 2322-5904 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Nanomedicine Journal |
| spelling | doaj-art-7d8cf06c526e4d7fbd23b7788a022bb72025-08-20T03:58:41ZengMashhad University of Medical SciencesNanomedicine Journal2322-30492322-59042025-01-01121425010.22038/nmj.2024.77144.187824903Enhancing peripheral nerve regeneration: A novel nanofibrous nerve conduit with bioactive poly(ɛ-caprolactone), collagen, and retinoic acid nanofiberMuhammad Aseer0Niloofar Nazeri1Nasrollah Tabatabaei2Zohreh Arabpour3Reza Faridi Majidi4Hossein Ghanbari5Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, IranCellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IranDepartment of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL, USADepartment of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, IranObjective(s): Peripheral nerve injury (PNI) is a critical clinical issue primarily caused by trauma. Tissue engineering approaches using nanofiber scaffolds have been extensively explored to improve material quality and create an environment resembling the natural extracellular matrix (ECM). Materials and Methods: In this study, we employed electrospinning technique to fabricate a composite scaffold comprising poly(ɛ-caprolactone) (PCL) and collagen (Col) loaded with all-trans retinoic acid (RA), a neural patterning and signaling chemical known to promote nerve regeneration. Results: The synthesized nanofiber scaffold exhibited a diameter of 391±79 nm and a tensile strength of 250±13 MPa, providing sufficient support for native peripheral nerve regeneration. The inclusion of Col enhanced the scaffold’s hydrophilic behavior (contact angle: 43±6°), ensuring stability in an aqueous solution. Moreover, the results demonstrated the proliferation and adhesion of nerve cells on the scaffold, aligning with the directions of the warp and weft of the nanofiber mat. Importantly, the scaffolds demonstrated non-toxicity, making them a promising substitute for the native ECM for enhanced cell attachment and proliferation. Finally, immune-histochemistry analyses further confirmed that the scaffolds supported the release and growth of neurites, promoting cell differentiation toward nerve repair. Conclusion: The RA-loaded scaffolds demonstrated the enhanced biocompatibility, supported neurite growth, and showed potential as a capable candidate for nerve regeneration.https://nmj.mums.ac.ir/article_24903_f0e1a4ca975176489810fa793dcfa484.pdfcollagenelectrospinningnerve cellsnerve regenerationpoly(ɛ-caprolactone)retinoic acid |
| spellingShingle | Muhammad Aseer Niloofar Nazeri Nasrollah Tabatabaei Zohreh Arabpour Reza Faridi Majidi Hossein Ghanbari Enhancing peripheral nerve regeneration: A novel nanofibrous nerve conduit with bioactive poly(ɛ-caprolactone), collagen, and retinoic acid nanofiber Nanomedicine Journal collagen electrospinning nerve cells nerve regeneration poly(ɛ-caprolactone) retinoic acid |
| title | Enhancing peripheral nerve regeneration: A novel nanofibrous nerve conduit with bioactive poly(ɛ-caprolactone), collagen, and retinoic acid nanofiber |
| title_full | Enhancing peripheral nerve regeneration: A novel nanofibrous nerve conduit with bioactive poly(ɛ-caprolactone), collagen, and retinoic acid nanofiber |
| title_fullStr | Enhancing peripheral nerve regeneration: A novel nanofibrous nerve conduit with bioactive poly(ɛ-caprolactone), collagen, and retinoic acid nanofiber |
| title_full_unstemmed | Enhancing peripheral nerve regeneration: A novel nanofibrous nerve conduit with bioactive poly(ɛ-caprolactone), collagen, and retinoic acid nanofiber |
| title_short | Enhancing peripheral nerve regeneration: A novel nanofibrous nerve conduit with bioactive poly(ɛ-caprolactone), collagen, and retinoic acid nanofiber |
| title_sort | enhancing peripheral nerve regeneration a novel nanofibrous nerve conduit with bioactive poly e caprolactone collagen and retinoic acid nanofiber |
| topic | collagen electrospinning nerve cells nerve regeneration poly(ɛ-caprolactone) retinoic acid |
| url | https://nmj.mums.ac.ir/article_24903_f0e1a4ca975176489810fa793dcfa484.pdf |
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