High Diversity of Tick-associated Microbiota from Five Tick Species in Yunnan, China

Ticks are obligate blood-sucking vectors for multiple zoonotic diseases. In this study, tick samples were collected from Yunnan Province, China, which is well-known as the “Global Biodiversity Hotspot” in the world. This study aimed to clarify the microbial populations, including pathogens, associat...

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Main Authors: Jie Zhang, Xue-Bing Ni, Zi-Yun Chen, Yun Zhang, Ran Wei, Zheng-Da Gong, Dai-Yun Zhu, Xiao-Ming Cui, Zong-Ti Shao, Hong-Bo Liu, Yu-Qiong Li, Jian Wang, Zi-Hou Gao, Xing-De Duan, Hang Jiang, Jia-Fu Jiang, Tommy Tsan-Yuk Lam, En-Jiong Huang, Na Jia, Chun-Hong Du
Format: Article
Language:English
Published: Compuscript Ltd 2023-07-01
Series:Zoonoses
Online Access:https://www.scienceopen.com/hosted-document?doi=10.15212/ZOONOSES-2023-0005
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author Jie Zhang
Xue-Bing Ni
Zi-Yun Chen
Yun Zhang
Ran Wei
Zheng-Da Gong
Dai-Yun Zhu
Xiao-Ming Cui
Zong-Ti Shao
Hong-Bo Liu
Yu-Qiong Li
Jian Wang
Zi-Hou Gao
Xing-De Duan
Hang Jiang
Jia-Fu Jiang
Tommy Tsan-Yuk Lam
En-Jiong Huang
Na Jia
Chun-Hong Du
author_facet Jie Zhang
Xue-Bing Ni
Zi-Yun Chen
Yun Zhang
Ran Wei
Zheng-Da Gong
Dai-Yun Zhu
Xiao-Ming Cui
Zong-Ti Shao
Hong-Bo Liu
Yu-Qiong Li
Jian Wang
Zi-Hou Gao
Xing-De Duan
Hang Jiang
Jia-Fu Jiang
Tommy Tsan-Yuk Lam
En-Jiong Huang
Na Jia
Chun-Hong Du
author_sort Jie Zhang
collection DOAJ
description Ticks are obligate blood-sucking vectors for multiple zoonotic diseases. In this study, tick samples were collected from Yunnan Province, China, which is well-known as the “Global Biodiversity Hotspot” in the world. This study aimed to clarify the microbial populations, including pathogens, associated with ticks and to identify the diversity of tick-borne microbiota in this region. The 16S rRNA full-length sequencing from pooled tick DNA samples and PCR amplification of pathogenic genera from individual samples were performed to understand tick-associated microbiota in this region. A total of 191 adult ticks of 5 tick species were included and revealed 11 phyla and 126 genera bacteria, including pathogenic Anaplasma , Ehrlichia , Candidatus Neoehrlichia, Rickettsia , Borrelia , and Babesia . Further identification suggested that Rickettsia sp. YN01 was a variant strain of Rickettsia spp. IG-1, but Rickettsia sp. YN02 and Rickettsia sp. YN03, were potentially two new SFGR species. This study revealed the complexity of ecological interactions between host and microbe and provided insight for the biological control of ticks. A high microbial diversity in ticks from Yunnan was identified, and more investigation should be undertaken to elucidate the pathogenicity in the area.
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spelling doaj-art-7d83cb3803c84a2da0ba4befcfc537a82025-08-20T02:36:06ZengCompuscript LtdZoonoses2737-74662737-74742023-07-013196710.15212/ZOONOSES-2023-0005High Diversity of Tick-associated Microbiota from Five Tick Species in Yunnan, ChinaJie ZhangXue-Bing NiZi-Yun ChenYun ZhangRan WeiZheng-Da GongDai-Yun ZhuXiao-Ming CuiZong-Ti ShaoHong-Bo LiuYu-Qiong LiJian WangZi-Hou GaoXing-De DuanHang JiangJia-Fu JiangTommy Tsan-Yuk LamEn-Jiong HuangNa JiaChun-Hong DuTicks are obligate blood-sucking vectors for multiple zoonotic diseases. In this study, tick samples were collected from Yunnan Province, China, which is well-known as the “Global Biodiversity Hotspot” in the world. This study aimed to clarify the microbial populations, including pathogens, associated with ticks and to identify the diversity of tick-borne microbiota in this region. The 16S rRNA full-length sequencing from pooled tick DNA samples and PCR amplification of pathogenic genera from individual samples were performed to understand tick-associated microbiota in this region. A total of 191 adult ticks of 5 tick species were included and revealed 11 phyla and 126 genera bacteria, including pathogenic Anaplasma , Ehrlichia , Candidatus Neoehrlichia, Rickettsia , Borrelia , and Babesia . Further identification suggested that Rickettsia sp. YN01 was a variant strain of Rickettsia spp. IG-1, but Rickettsia sp. YN02 and Rickettsia sp. YN03, were potentially two new SFGR species. This study revealed the complexity of ecological interactions between host and microbe and provided insight for the biological control of ticks. A high microbial diversity in ticks from Yunnan was identified, and more investigation should be undertaken to elucidate the pathogenicity in the area.https://www.scienceopen.com/hosted-document?doi=10.15212/ZOONOSES-2023-0005
spellingShingle Jie Zhang
Xue-Bing Ni
Zi-Yun Chen
Yun Zhang
Ran Wei
Zheng-Da Gong
Dai-Yun Zhu
Xiao-Ming Cui
Zong-Ti Shao
Hong-Bo Liu
Yu-Qiong Li
Jian Wang
Zi-Hou Gao
Xing-De Duan
Hang Jiang
Jia-Fu Jiang
Tommy Tsan-Yuk Lam
En-Jiong Huang
Na Jia
Chun-Hong Du
High Diversity of Tick-associated Microbiota from Five Tick Species in Yunnan, China
Zoonoses
title High Diversity of Tick-associated Microbiota from Five Tick Species in Yunnan, China
title_full High Diversity of Tick-associated Microbiota from Five Tick Species in Yunnan, China
title_fullStr High Diversity of Tick-associated Microbiota from Five Tick Species in Yunnan, China
title_full_unstemmed High Diversity of Tick-associated Microbiota from Five Tick Species in Yunnan, China
title_short High Diversity of Tick-associated Microbiota from Five Tick Species in Yunnan, China
title_sort high diversity of tick associated microbiota from five tick species in yunnan china
url https://www.scienceopen.com/hosted-document?doi=10.15212/ZOONOSES-2023-0005
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