Effect of collagen-based scaffolds with hydroxyapatite on the repair of cartilage defects in the rabbit knee joint
Abstract Background The repair of articular cartilage defects is always a significant clinical challenge in joint treatment. Therefore, the aim of this study was to investigate that the ColII-HA-CS-HAP scaffolds with BMSCs could repair cartilage defects of knee. Methods Bone marrow mesenchymal stem...
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| Format: | Article |
| Language: | English |
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BMC
2024-12-01
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| Series: | Journal of Orthopaedic Surgery and Research |
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| Online Access: | https://doi.org/10.1186/s13018-024-05323-5 |
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| _version_ | 1849220661325594624 |
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| author | Xiaoliang He Qiuping Han Yuxin Zhang Huan Zhang Jun Liu Xiaohui Zhou |
| author_facet | Xiaoliang He Qiuping Han Yuxin Zhang Huan Zhang Jun Liu Xiaohui Zhou |
| author_sort | Xiaoliang He |
| collection | DOAJ |
| description | Abstract Background The repair of articular cartilage defects is always a significant clinical challenge in joint treatment. Therefore, the aim of this study was to investigate that the ColII-HA-CS-HAP scaffolds with BMSCs could repair cartilage defects of knee. Methods Bone marrow mesenchymal stem cells (BMSCs) were extracted from rabbits, identified using immunofluorescence staining, and successfully induced into chondrocytes. Type II collagen (ColII) was isolated from bovine cartilage and constructed into scaffolds with hyaluronic acid, chondroitin sulfate, and hydroxyapatite. Then BMSCs were seeded on the ColII-HA-CS-HAP scaffold to detect biocompatibility. Results The results of DAPI fluorescence staining showed that the number of BMSCs on the ColII-HA-CS-HAP scaffolds increased rapidly after culturing for 12 d. The rabbit knee cartilage defect model with a diameter of approximately 3 mm and a thickness of approximately 4 mm was selected to evaluate the regenerative potential of the scaffolds using histological and immunohistochemical analyses. At 6 months, the regenerated cartilage in the ColII-HA-CS-HAP scaffolds with BMSCs was more similar to that of native cartilage than the ColII-HA-CS-HAP scaffold group. Conclusions Our study proved that the ColII-HA-CS-HAP scaffolds with differentiated BMSCs can produce an excellent healing response and repair cartilage defects successfully in a rabbit model. |
| format | Article |
| id | doaj-art-7d8153cb8e8d45d39efc6acf466162b0 |
| institution | Kabale University |
| issn | 1749-799X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj-art-7d8153cb8e8d45d39efc6acf466162b02024-12-08T12:37:47ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2024-12-0119111210.1186/s13018-024-05323-5Effect of collagen-based scaffolds with hydroxyapatite on the repair of cartilage defects in the rabbit knee jointXiaoliang He0Qiuping Han1Yuxin Zhang2Huan Zhang3Jun Liu4Xiaohui Zhou5College of Food Science and Biology, Hebei University of Science and TechnologyCollege of Food Science and Biology, Hebei University of Science and TechnologyCollege of Food Science and Biology, Hebei University of Science and TechnologyCollege of Food Science and Biology, Hebei University of Science and TechnologyCollege of Food and Biotechnology, Qiqihar UniversityCollege of Food Science and Biology, Hebei University of Science and TechnologyAbstract Background The repair of articular cartilage defects is always a significant clinical challenge in joint treatment. Therefore, the aim of this study was to investigate that the ColII-HA-CS-HAP scaffolds with BMSCs could repair cartilage defects of knee. Methods Bone marrow mesenchymal stem cells (BMSCs) were extracted from rabbits, identified using immunofluorescence staining, and successfully induced into chondrocytes. Type II collagen (ColII) was isolated from bovine cartilage and constructed into scaffolds with hyaluronic acid, chondroitin sulfate, and hydroxyapatite. Then BMSCs were seeded on the ColII-HA-CS-HAP scaffold to detect biocompatibility. Results The results of DAPI fluorescence staining showed that the number of BMSCs on the ColII-HA-CS-HAP scaffolds increased rapidly after culturing for 12 d. The rabbit knee cartilage defect model with a diameter of approximately 3 mm and a thickness of approximately 4 mm was selected to evaluate the regenerative potential of the scaffolds using histological and immunohistochemical analyses. At 6 months, the regenerated cartilage in the ColII-HA-CS-HAP scaffolds with BMSCs was more similar to that of native cartilage than the ColII-HA-CS-HAP scaffold group. Conclusions Our study proved that the ColII-HA-CS-HAP scaffolds with differentiated BMSCs can produce an excellent healing response and repair cartilage defects successfully in a rabbit model.https://doi.org/10.1186/s13018-024-05323-5BMSCsType II collagen (ColII)Cartilage scaffoldCartilage defect |
| spellingShingle | Xiaoliang He Qiuping Han Yuxin Zhang Huan Zhang Jun Liu Xiaohui Zhou Effect of collagen-based scaffolds with hydroxyapatite on the repair of cartilage defects in the rabbit knee joint Journal of Orthopaedic Surgery and Research BMSCs Type II collagen (ColII) Cartilage scaffold Cartilage defect |
| title | Effect of collagen-based scaffolds with hydroxyapatite on the repair of cartilage defects in the rabbit knee joint |
| title_full | Effect of collagen-based scaffolds with hydroxyapatite on the repair of cartilage defects in the rabbit knee joint |
| title_fullStr | Effect of collagen-based scaffolds with hydroxyapatite on the repair of cartilage defects in the rabbit knee joint |
| title_full_unstemmed | Effect of collagen-based scaffolds with hydroxyapatite on the repair of cartilage defects in the rabbit knee joint |
| title_short | Effect of collagen-based scaffolds with hydroxyapatite on the repair of cartilage defects in the rabbit knee joint |
| title_sort | effect of collagen based scaffolds with hydroxyapatite on the repair of cartilage defects in the rabbit knee joint |
| topic | BMSCs Type II collagen (ColII) Cartilage scaffold Cartilage defect |
| url | https://doi.org/10.1186/s13018-024-05323-5 |
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