Evaluating the Wound Healing Activity of Fabricated Stingless Bee Honey Hydrogels in an Animal Model

Introduction: Honey is a well-established treatment for wound healing and had been used for ages. However, there are limitations when it is directly applied to the wound such as inconsistent dosage and pain during dressing changes. The incorporation of honey into hydrogel could overcome these probl...

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Main Authors: Mohd Azri Abd Jalil, Hazrina Hadi, Muhammad Lokman Md Isa, Umar Azhan, Kamarul Ariffin Khalid, Md Abul Barkat
Format: Article
Language:English
Published: IIUM Press, International Islamic University Malaysia 2025-07-01
Series:Journal of Pharmacy
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Online Access:https://journals.iium.edu.my/ktn/index.php/jp/article/view/399
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author Mohd Azri Abd Jalil
Hazrina Hadi
Muhammad Lokman Md Isa
Umar Azhan
Kamarul Ariffin Khalid
Md Abul Barkat
author_facet Mohd Azri Abd Jalil
Hazrina Hadi
Muhammad Lokman Md Isa
Umar Azhan
Kamarul Ariffin Khalid
Md Abul Barkat
author_sort Mohd Azri Abd Jalil
collection DOAJ
description Introduction: Honey is a well-established treatment for wound healing and had been used for ages. However, there are limitations when it is directly applied to the wound such as inconsistent dosage and pain during dressing changes. The incorporation of honey into hydrogel could overcome these problems. The study is aimed at developing a suitable honey-based hydrogel formulation for wound healing application by using systematic experimental design from response surface methods (RSM). Methods: The hydrogel’s base was made from polyvinyl alcohol (PVA), polyethylene glycol (PEG), glycerol and agar. A two-level factorial design was selected to screen the factors followed by centred composite design for optimization. The characterizations of the optimized formulation were observed in term of hydrophilicity and rheological. The optimized formulation was further assessed in an in vivo wound healing study in New Zealand albino rabbits. Results: PEG and agar concentration was found to be the most important process variable based on the screening result. The optimised hydrogel has a good hydrophilicity ability and rheological property. In in vivo healing study, the healing in the honey incorporated hydrogel treated group was significantly faster than the no treatment group, as demonstrated in wound closure percentage and histological assessment. From the results, the wound in the honey hydrogel treatment group has entered the remodelling phase compared with the control group that was still in the proliferation phase. Conclusion: Based on all of these results, stingless bee honey incorporated hydrogel has a promising application as an efficient wound dressing.
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spelling doaj-art-7d7cf8a1809c48bf94ee1246e96071bc2025-08-20T03:16:07ZengIIUM Press, International Islamic University MalaysiaJournal of Pharmacy2773-56642025-07-0152Evaluating the Wound Healing Activity of Fabricated Stingless Bee Honey Hydrogels in an Animal Model Mohd Azri Abd JalilHazrina Hadi0Muhammad Lokman Md IsaUmar AzhanKamarul Ariffin KhalidMd Abul BarkatInternational Islamic University Malaysia Introduction: Honey is a well-established treatment for wound healing and had been used for ages. However, there are limitations when it is directly applied to the wound such as inconsistent dosage and pain during dressing changes. The incorporation of honey into hydrogel could overcome these problems. The study is aimed at developing a suitable honey-based hydrogel formulation for wound healing application by using systematic experimental design from response surface methods (RSM). Methods: The hydrogel’s base was made from polyvinyl alcohol (PVA), polyethylene glycol (PEG), glycerol and agar. A two-level factorial design was selected to screen the factors followed by centred composite design for optimization. The characterizations of the optimized formulation were observed in term of hydrophilicity and rheological. The optimized formulation was further assessed in an in vivo wound healing study in New Zealand albino rabbits. Results: PEG and agar concentration was found to be the most important process variable based on the screening result. The optimised hydrogel has a good hydrophilicity ability and rheological property. In in vivo healing study, the healing in the honey incorporated hydrogel treated group was significantly faster than the no treatment group, as demonstrated in wound closure percentage and histological assessment. From the results, the wound in the honey hydrogel treatment group has entered the remodelling phase compared with the control group that was still in the proliferation phase. Conclusion: Based on all of these results, stingless bee honey incorporated hydrogel has a promising application as an efficient wound dressing. https://journals.iium.edu.my/ktn/index.php/jp/article/view/399HydrogelStingless Bee HoneyWound Healing
spellingShingle Mohd Azri Abd Jalil
Hazrina Hadi
Muhammad Lokman Md Isa
Umar Azhan
Kamarul Ariffin Khalid
Md Abul Barkat
Evaluating the Wound Healing Activity of Fabricated Stingless Bee Honey Hydrogels in an Animal Model
Journal of Pharmacy
Hydrogel
Stingless Bee Honey
Wound Healing
title Evaluating the Wound Healing Activity of Fabricated Stingless Bee Honey Hydrogels in an Animal Model
title_full Evaluating the Wound Healing Activity of Fabricated Stingless Bee Honey Hydrogels in an Animal Model
title_fullStr Evaluating the Wound Healing Activity of Fabricated Stingless Bee Honey Hydrogels in an Animal Model
title_full_unstemmed Evaluating the Wound Healing Activity of Fabricated Stingless Bee Honey Hydrogels in an Animal Model
title_short Evaluating the Wound Healing Activity of Fabricated Stingless Bee Honey Hydrogels in an Animal Model
title_sort evaluating the wound healing activity of fabricated stingless bee honey hydrogels in an animal model
topic Hydrogel
Stingless Bee Honey
Wound Healing
url https://journals.iium.edu.my/ktn/index.php/jp/article/view/399
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