Effect of radiation therapy on the dentin bond strength of a universal adhesive

Abstract Background Successful restorative procedures, particularly for patients undergoing head and neck radiotherapy, rely on a robust adhesive interface. Since radiotherapy may alter dental tissues and compromise bonding, this study evaluated how the timing of radiation exposure affects the micro...

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Main Authors: Pipop Saikaew, P Hannongbua, P Jianratanajit, I Wongkertprayot, P Chaiklahan, N Srimaneekarn, A Prayongrat, P Eamsa-ard, AFMA Chowdhury, K Katekovit, S Hidehiko
Format: Article
Language:English
Published: BMC 2025-05-01
Series:BMC Oral Health
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Online Access:https://doi.org/10.1186/s12903-025-06226-5
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author Pipop Saikaew
P Hannongbua
P Jianratanajit
I Wongkertprayot
P Chaiklahan
N Srimaneekarn
A Prayongrat
P Eamsa-ard
AFMA Chowdhury
K Katekovit
S Hidehiko
author_facet Pipop Saikaew
P Hannongbua
P Jianratanajit
I Wongkertprayot
P Chaiklahan
N Srimaneekarn
A Prayongrat
P Eamsa-ard
AFMA Chowdhury
K Katekovit
S Hidehiko
author_sort Pipop Saikaew
collection DOAJ
description Abstract Background Successful restorative procedures, particularly for patients undergoing head and neck radiotherapy, rely on a robust adhesive interface. Since radiotherapy may alter dental tissues and compromise bonding, this study evaluated how the timing of radiation exposure affects the microtensile bond strength (µTBS) of a universal adhesive to dentin with different application modes. Methods Forty-two human third molars were used in this study. Mid-coronal dentin was prepared using a low-speed saw and polished with silicon carbide paper. The samples were randomly divided into 3 groups (n = 6) according to the timing of radiation exposure (no radiation, control; radiation after restoration, F-RT; and radiation before restoration, RT-F). A single radiation dose of 70 Gy was administered to the samples after restoration (F-RT) and before restoration (RT-F). The samples were further divided into 2 subgroups according to the application mode of a universal adhesive (Single Bond Universal adhesive, 3 M Oral Care, St. Paul, MN, USA): etch-and-rinse (ER) or self-etch (SE) mode. After adhesive application, the resin composite was constructed and stored in distilled water at 37 °C for 24 h. Six resin-bonded teeth per group were processed for the µTBS test. The data were analyzed by two-way ANOVA followed by Duncan’s test (p < 0.05). Fractured surfaces were observed under a scanning electron microscope (SEM). Additional teeth were prepared for resin‒dentin interface observation (n = 1) and observed using an SEM. Results The application mode of the universal adhesive had no influence on the µTBS of dentin, regardless of irradiation (p = 0.670). The µTBS values of the control groups were not significantly different from those of the F-RT group but were significantly higher than those of the RT-F group for both application modes. The resin‒dentin interfaces of the samples were similar among the control, F-RT and RT-F groups. More abundant and longer resin tags were observed when the universal adhesive was bonded in ER mode. Conclusion Compared with radiation after restoration, radiation before restoration resulted in an inferior bond strength. The application mode of a universal adhesive had no effect on the bond strength. Clinical significance Restoration prior to the radiation procedure is an advisable strategy.
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spelling doaj-art-7d7c4ec2e6c947a4abd6ebdc3f87b9c42025-08-20T02:00:10ZengBMCBMC Oral Health1472-68312025-05-012511810.1186/s12903-025-06226-5Effect of radiation therapy on the dentin bond strength of a universal adhesivePipop Saikaew0P Hannongbua1P Jianratanajit2I Wongkertprayot3P Chaiklahan4N Srimaneekarn5A Prayongrat6P Eamsa-ard7AFMA Chowdhury8K Katekovit9S Hidehiko10Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol UniversityFaculty of Dentistry, Mahidol UniversityFaculty of Dentistry, Mahidol UniversityFaculty of Dentistry, Mahidol UniversityFaculty of Dentistry, Mahidol UniversityDepartment of Anatomy, Faculty of Dentistry, Mahidol UniversityDivision of Radiation Oncology, Department of Radiology, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial HospitalDepartment of Operative Dentistry, College of Dental Medicine, Rangsit UniversityDepartment of Conservative Dentistry and Endodontics, Sapporo Dental College and HospitalFaculty of Dentistry, Mahidol UniversityDepartment of Restorative Dentistry, Faculty of Dental Medicine, Hokkaido UniversityAbstract Background Successful restorative procedures, particularly for patients undergoing head and neck radiotherapy, rely on a robust adhesive interface. Since radiotherapy may alter dental tissues and compromise bonding, this study evaluated how the timing of radiation exposure affects the microtensile bond strength (µTBS) of a universal adhesive to dentin with different application modes. Methods Forty-two human third molars were used in this study. Mid-coronal dentin was prepared using a low-speed saw and polished with silicon carbide paper. The samples were randomly divided into 3 groups (n = 6) according to the timing of radiation exposure (no radiation, control; radiation after restoration, F-RT; and radiation before restoration, RT-F). A single radiation dose of 70 Gy was administered to the samples after restoration (F-RT) and before restoration (RT-F). The samples were further divided into 2 subgroups according to the application mode of a universal adhesive (Single Bond Universal adhesive, 3 M Oral Care, St. Paul, MN, USA): etch-and-rinse (ER) or self-etch (SE) mode. After adhesive application, the resin composite was constructed and stored in distilled water at 37 °C for 24 h. Six resin-bonded teeth per group were processed for the µTBS test. The data were analyzed by two-way ANOVA followed by Duncan’s test (p < 0.05). Fractured surfaces were observed under a scanning electron microscope (SEM). Additional teeth were prepared for resin‒dentin interface observation (n = 1) and observed using an SEM. Results The application mode of the universal adhesive had no influence on the µTBS of dentin, regardless of irradiation (p = 0.670). The µTBS values of the control groups were not significantly different from those of the F-RT group but were significantly higher than those of the RT-F group for both application modes. The resin‒dentin interfaces of the samples were similar among the control, F-RT and RT-F groups. More abundant and longer resin tags were observed when the universal adhesive was bonded in ER mode. Conclusion Compared with radiation after restoration, radiation before restoration resulted in an inferior bond strength. The application mode of a universal adhesive had no effect on the bond strength. Clinical significance Restoration prior to the radiation procedure is an advisable strategy.https://doi.org/10.1186/s12903-025-06226-5Universal adhesiveDentinMicrotensile bond strengthRadiation
spellingShingle Pipop Saikaew
P Hannongbua
P Jianratanajit
I Wongkertprayot
P Chaiklahan
N Srimaneekarn
A Prayongrat
P Eamsa-ard
AFMA Chowdhury
K Katekovit
S Hidehiko
Effect of radiation therapy on the dentin bond strength of a universal adhesive
BMC Oral Health
Universal adhesive
Dentin
Microtensile bond strength
Radiation
title Effect of radiation therapy on the dentin bond strength of a universal adhesive
title_full Effect of radiation therapy on the dentin bond strength of a universal adhesive
title_fullStr Effect of radiation therapy on the dentin bond strength of a universal adhesive
title_full_unstemmed Effect of radiation therapy on the dentin bond strength of a universal adhesive
title_short Effect of radiation therapy on the dentin bond strength of a universal adhesive
title_sort effect of radiation therapy on the dentin bond strength of a universal adhesive
topic Universal adhesive
Dentin
Microtensile bond strength
Radiation
url https://doi.org/10.1186/s12903-025-06226-5
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