M2-like macrophage-derived exosomes inhibit osteoclastogenesis via releasing miR-1227-5p
Macrophages play a pivotal role in regulating inflammatory response in periodontitis, a condition characterized by excessive osteoclast differentiation. This study aimed to investigate whether exosomes derived from M2 macrophages regulate osteoclast differentiation and to identify the underlying mol...
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Elsevier
2025-01-01
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| Series: | Immunobiology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0171298524000792 |
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| author | Shan Chen Jian Liu Lilei Zhu |
| author_facet | Shan Chen Jian Liu Lilei Zhu |
| author_sort | Shan Chen |
| collection | DOAJ |
| description | Macrophages play a pivotal role in regulating inflammatory response in periodontitis, a condition characterized by excessive osteoclast differentiation. This study aimed to investigate whether exosomes derived from M2 macrophages regulate osteoclast differentiation and to identify the underlying molecular mechanisms. Exosomes were isolated from M2 macrophages and used to treat osteoclasts. Osteoclastogenesis was assessed using tartrate-resistant acid phosphatase staining and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The molecular mechanism was evaluated using microarray analysis, RT-qPCR, dual-luciferase reporter analysis, and RNA pull-down assay. The results showed that exosomes from M2 macrophages inhibited receptor activator of nuclear factor κ-B ligand (RANKL)-induced osteoclast differentiation. Additionally, miR-1227-5p expression in osteoclasts was increased after treatment with exosomes, and inhibition of miR-1227-5p counteracted the suppressive effects of exosomes on osteoclastogenesis. Moreover, OSCAR is a target of miR-1227-5p. In conclusion, exosomal miR-1227-5p suppresses osteoclast differentiation, potentially via targeting OSCAR. These findings provide new insights into the pathogenesis of periodontitis. |
| format | Article |
| id | doaj-art-7d79c850747347e3beb8d6206e3900ec |
| institution | Kabale University |
| issn | 0171-2985 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Immunobiology |
| spelling | doaj-art-7d79c850747347e3beb8d6206e3900ec2025-01-27T04:21:41ZengElsevierImmunobiology0171-29852025-01-012301152861M2-like macrophage-derived exosomes inhibit osteoclastogenesis via releasing miR-1227-5pShan Chen0Jian Liu1Lilei Zhu2Department of Periodontology, Changsha Stomatological Hospital, No. 389, Youyi Road, Tianxin District, Changsha 410004, ChinaDepartment of Periodontology, Changsha Stomatological Hospital, No. 389, Youyi Road, Tianxin District, Changsha 410004, ChinaCorresponding author.; Department of Periodontology, Changsha Stomatological Hospital, No. 389, Youyi Road, Tianxin District, Changsha 410004, ChinaMacrophages play a pivotal role in regulating inflammatory response in periodontitis, a condition characterized by excessive osteoclast differentiation. This study aimed to investigate whether exosomes derived from M2 macrophages regulate osteoclast differentiation and to identify the underlying molecular mechanisms. Exosomes were isolated from M2 macrophages and used to treat osteoclasts. Osteoclastogenesis was assessed using tartrate-resistant acid phosphatase staining and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The molecular mechanism was evaluated using microarray analysis, RT-qPCR, dual-luciferase reporter analysis, and RNA pull-down assay. The results showed that exosomes from M2 macrophages inhibited receptor activator of nuclear factor κ-B ligand (RANKL)-induced osteoclast differentiation. Additionally, miR-1227-5p expression in osteoclasts was increased after treatment with exosomes, and inhibition of miR-1227-5p counteracted the suppressive effects of exosomes on osteoclastogenesis. Moreover, OSCAR is a target of miR-1227-5p. In conclusion, exosomal miR-1227-5p suppresses osteoclast differentiation, potentially via targeting OSCAR. These findings provide new insights into the pathogenesis of periodontitis.http://www.sciencedirect.com/science/article/pii/S0171298524000792M2 macrophageOsteoclast differentiationExosomemiR-1227-5pOSCAR |
| spellingShingle | Shan Chen Jian Liu Lilei Zhu M2-like macrophage-derived exosomes inhibit osteoclastogenesis via releasing miR-1227-5p Immunobiology M2 macrophage Osteoclast differentiation Exosome miR-1227-5p OSCAR |
| title | M2-like macrophage-derived exosomes inhibit osteoclastogenesis via releasing miR-1227-5p |
| title_full | M2-like macrophage-derived exosomes inhibit osteoclastogenesis via releasing miR-1227-5p |
| title_fullStr | M2-like macrophage-derived exosomes inhibit osteoclastogenesis via releasing miR-1227-5p |
| title_full_unstemmed | M2-like macrophage-derived exosomes inhibit osteoclastogenesis via releasing miR-1227-5p |
| title_short | M2-like macrophage-derived exosomes inhibit osteoclastogenesis via releasing miR-1227-5p |
| title_sort | m2 like macrophage derived exosomes inhibit osteoclastogenesis via releasing mir 1227 5p |
| topic | M2 macrophage Osteoclast differentiation Exosome miR-1227-5p OSCAR |
| url | http://www.sciencedirect.com/science/article/pii/S0171298524000792 |
| work_keys_str_mv | AT shanchen m2likemacrophagederivedexosomesinhibitosteoclastogenesisviareleasingmir12275p AT jianliu m2likemacrophagederivedexosomesinhibitosteoclastogenesisviareleasingmir12275p AT lileizhu m2likemacrophagederivedexosomesinhibitosteoclastogenesisviareleasingmir12275p |