A Unique THN Motif Is Critical for Enabling Efficient C‐Terminal Traceless Cleavage

A Unique THN Motif Is Critical for Enabling Efficient C‐Terminal Traceless Cleavage

Abstract Traceless protein cleavage is a significant challenge in intein application, as most common inteins studied today are not both active and promiscuous. In this study, the intein gp41‐1 is engineered, which demonstrates the most efficient traceless cleavage reported to date and shows high com...

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Bibliographic Details
Main Authors: Ruocheng Gu, Yunuo Lin, Rouyu Di, Tongtong Zhou, Tingwen Fan, Wei Li, Lili Miao, Huaiyi Yang
Format: Article
Language:English
Published: Wiley 2025-07-01
Series:Advanced Science
Subjects:
gp41‐1
high cleavage activity
traceless cleavage
THN motif
Online Access:https://doi.org/10.1002/advs.202501991
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