QTL mapping for branch- and leaf-related traits with a high-density SNP genetic map in litchi (Litchi chinensis Sonn.)
Litchi (Litchi chinensis Sonn.), an important fruit tree in tropical and subtropical regions, possesses substantial economic value. The branch- and leaf-related traits of litchi have a significant impact on litchi yield and quality. However, due to limitations such as the density of the genetic link...
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KeAi Communications Co., Ltd.
2025-07-01
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| Series: | Horticultural Plant Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014124001547 |
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| author | Wei Hu Fang Li Huanling Li Lei Zhang Rupeng Cai Qiying Lin Yao Li Xiaoyun Qin Jiabao Wang |
| author_facet | Wei Hu Fang Li Huanling Li Lei Zhang Rupeng Cai Qiying Lin Yao Li Xiaoyun Qin Jiabao Wang |
| author_sort | Wei Hu |
| collection | DOAJ |
| description | Litchi (Litchi chinensis Sonn.), an important fruit tree in tropical and subtropical regions, possesses substantial economic value. The branch- and leaf-related traits of litchi have a significant impact on litchi yield and quality. However, due to limitations such as the density of the genetic linkage map, there have been few studies on mapping QTLs of branch- and leaf-related traits. In this study, a high-density genetic map was constructed by next-generation sequencing (NGS) using an F1 population of 264 progenies, derived from the cross between the cultivars ‘Sanyuehong’ and ‘Ziniangxi’. A total of 2574 high-quality BINs (binomial intervals) were obtained, and a genetic linkage map was constructed with a total length of 1753.3 cM and an average marker distance of 0.68 cM. With the genetic map and the phenotyping of single leaf length (SLL), single leaf width (SLW), leaf shape index (LSI), weight of specific leaf (WSL), petiole length (PL) and compound leaf length (CLL) measured in three seasons, 11, 9, 9, 10, 9 and 12 QTLs were detected for SLL, SLW, WSL, LSI, PL and CLL traits, respectively. Among these QTLs, five QTLs were consistently detected in two seasons and 12 pleiotropic QTLs were identified for at least two traits. These findings will provide new insights for the gene cloning for branch- and leaf-related traits as well as marker-assisted selection (MAS). |
| format | Article |
| id | doaj-art-7d6777ad08ae447da2c7e2c7e2b3cbcc |
| institution | Kabale University |
| issn | 2468-0141 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj-art-7d6777ad08ae447da2c7e2c7e2b3cbcc2025-08-20T03:50:05ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412025-07-011141541155010.1016/j.hpj.2024.04.005QTL mapping for branch- and leaf-related traits with a high-density SNP genetic map in litchi (Litchi chinensis Sonn.)Wei Hu0Fang Li1Huanling Li2Lei Zhang3Rupeng Cai4Qiying Lin5Yao Li6Xiaoyun Qin7Jiabao Wang8Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, ChinaEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, ChinaEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, ChinaTropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, ChinaEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, ChinaEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, ChinaTropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, ChinaEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, ChinaTropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China; Corresponding authorLitchi (Litchi chinensis Sonn.), an important fruit tree in tropical and subtropical regions, possesses substantial economic value. The branch- and leaf-related traits of litchi have a significant impact on litchi yield and quality. However, due to limitations such as the density of the genetic linkage map, there have been few studies on mapping QTLs of branch- and leaf-related traits. In this study, a high-density genetic map was constructed by next-generation sequencing (NGS) using an F1 population of 264 progenies, derived from the cross between the cultivars ‘Sanyuehong’ and ‘Ziniangxi’. A total of 2574 high-quality BINs (binomial intervals) were obtained, and a genetic linkage map was constructed with a total length of 1753.3 cM and an average marker distance of 0.68 cM. With the genetic map and the phenotyping of single leaf length (SLL), single leaf width (SLW), leaf shape index (LSI), weight of specific leaf (WSL), petiole length (PL) and compound leaf length (CLL) measured in three seasons, 11, 9, 9, 10, 9 and 12 QTLs were detected for SLL, SLW, WSL, LSI, PL and CLL traits, respectively. Among these QTLs, five QTLs were consistently detected in two seasons and 12 pleiotropic QTLs were identified for at least two traits. These findings will provide new insights for the gene cloning for branch- and leaf-related traits as well as marker-assisted selection (MAS).http://www.sciencedirect.com/science/article/pii/S2468014124001547LitchiBranch- and leaf-related traitsHigh-density genetic mapPleiotropic QTLs |
| spellingShingle | Wei Hu Fang Li Huanling Li Lei Zhang Rupeng Cai Qiying Lin Yao Li Xiaoyun Qin Jiabao Wang QTL mapping for branch- and leaf-related traits with a high-density SNP genetic map in litchi (Litchi chinensis Sonn.) Horticultural Plant Journal Litchi Branch- and leaf-related traits High-density genetic map Pleiotropic QTLs |
| title | QTL mapping for branch- and leaf-related traits with a high-density SNP genetic map in litchi (Litchi chinensis Sonn.) |
| title_full | QTL mapping for branch- and leaf-related traits with a high-density SNP genetic map in litchi (Litchi chinensis Sonn.) |
| title_fullStr | QTL mapping for branch- and leaf-related traits with a high-density SNP genetic map in litchi (Litchi chinensis Sonn.) |
| title_full_unstemmed | QTL mapping for branch- and leaf-related traits with a high-density SNP genetic map in litchi (Litchi chinensis Sonn.) |
| title_short | QTL mapping for branch- and leaf-related traits with a high-density SNP genetic map in litchi (Litchi chinensis Sonn.) |
| title_sort | qtl mapping for branch and leaf related traits with a high density snp genetic map in litchi litchi chinensis sonn |
| topic | Litchi Branch- and leaf-related traits High-density genetic map Pleiotropic QTLs |
| url | http://www.sciencedirect.com/science/article/pii/S2468014124001547 |
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