Ultrasound irradiation activates purine metabolism and mitochondrial respiration via the MAPK signaling pathway in myotubes
Background: Pulsed ultrasound (US) is widely used both as a diagnostic imaging tool and a therapeutic approach. However, many of the mechanisms underlying the therapeutic effects of non-thermal US remain unclear, especially in skeletal muscles, which play a crucial role in the body's metabolism...
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Elsevier
2025-06-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580825000718 |
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| author | Xiaoqi Ma Noriaki Maeshige Atomu Yamaguchi Yunfei Fu Jihao Xing Qingcheng Guo Hao Lin Fuwen Lu Hiroyo Kondo Hidemi Fujino |
| author_facet | Xiaoqi Ma Noriaki Maeshige Atomu Yamaguchi Yunfei Fu Jihao Xing Qingcheng Guo Hao Lin Fuwen Lu Hiroyo Kondo Hidemi Fujino |
| author_sort | Xiaoqi Ma |
| collection | DOAJ |
| description | Background: Pulsed ultrasound (US) is widely used both as a diagnostic imaging tool and a therapeutic approach. However, many of the mechanisms underlying the therapeutic effects of non-thermal US remain unclear, especially in skeletal muscles, which play a crucial role in the body's metabolism. The aim of this study was to investigate the effects of US on myotubes. Methods: In this study, C2C12 myoblasts were utilized. After differentiating into myotubes, the cells were exposed to US irradiation at an intensity of 3.0 W/cm2, with a 20 % duty cycle, an acoustic frequency of 1 MHz, and a pulse repetition frequency of 100 Hz for 5 min. The cells were then collected and analyzed for genomic and metabolomic alterations, as well as mitochondrial function. Results: Cell viability remained unaffected after US irradiation. The mitogen-activated protein kinase (MAPK) signaling pathway was the most activated, while the expression of various RNAs was significantly altered. Purine metabolism was highly activated, with an increase in the abundance of metabolites associated with this pathway. Furthermore, mitochondrial respiration in the myotubes increased following US irradiation. Conclusion: This study investigated the impact of US irradiation on myotubes using genomic analysis, metabolomic analysis, and mitochondrial function. US irradiation activated the MAPK signaling pathway, which in turn enhanced purine metabolism and improved mitochondrial respiration. |
| format | Article |
| id | doaj-art-7d65995bd2a04dbf9da42e0dfab5ebfd |
| institution | OA Journals |
| issn | 2405-5808 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj-art-7d65995bd2a04dbf9da42e0dfab5ebfd2025-08-20T02:17:13ZengElsevierBiochemistry and Biophysics Reports2405-58082025-06-014210198410.1016/j.bbrep.2025.101984Ultrasound irradiation activates purine metabolism and mitochondrial respiration via the MAPK signaling pathway in myotubesXiaoqi Ma0Noriaki Maeshige1Atomu Yamaguchi2Yunfei Fu3Jihao Xing4Qingcheng Guo5Hao Lin6Fuwen Lu7Hiroyo Kondo8Hidemi Fujino9Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences, Kobe, JapanDepartment of Rehabilitation Science, Kobe University Graduate School of Health Sciences, Kobe, Japan; Corresponding author. Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences, 7-10-2 Tomogaoka, 654-0142, Kobe, Hyogo, Japan.Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences, Kobe, JapanDepartment of Rehabilitation Science, Kobe University Graduate School of Health Sciences, Kobe, JapanDepartment of Rehabilitation Science, Kobe University Graduate School of Health Sciences, Kobe, JapanDepartment of Rehabilitation Science, Kobe University Graduate School of Health Sciences, Kobe, JapanDepartment of Rehabilitation Science, Kobe University Graduate School of Health Sciences, Kobe, JapanShanghai Yangzhi Rehabilitation Hospital (Shanghai Sunshine Rehabilitation Center), Tongji University School of Medicine, Shanghai, ChinaFaculty of Health and Nutrition, Shubun University, Ichinomiya, JapanDepartment of Rehabilitation Science, Kobe University Graduate School of Health Sciences, Kobe, JapanBackground: Pulsed ultrasound (US) is widely used both as a diagnostic imaging tool and a therapeutic approach. However, many of the mechanisms underlying the therapeutic effects of non-thermal US remain unclear, especially in skeletal muscles, which play a crucial role in the body's metabolism. The aim of this study was to investigate the effects of US on myotubes. Methods: In this study, C2C12 myoblasts were utilized. After differentiating into myotubes, the cells were exposed to US irradiation at an intensity of 3.0 W/cm2, with a 20 % duty cycle, an acoustic frequency of 1 MHz, and a pulse repetition frequency of 100 Hz for 5 min. The cells were then collected and analyzed for genomic and metabolomic alterations, as well as mitochondrial function. Results: Cell viability remained unaffected after US irradiation. The mitogen-activated protein kinase (MAPK) signaling pathway was the most activated, while the expression of various RNAs was significantly altered. Purine metabolism was highly activated, with an increase in the abundance of metabolites associated with this pathway. Furthermore, mitochondrial respiration in the myotubes increased following US irradiation. Conclusion: This study investigated the impact of US irradiation on myotubes using genomic analysis, metabolomic analysis, and mitochondrial function. US irradiation activated the MAPK signaling pathway, which in turn enhanced purine metabolism and improved mitochondrial respiration.http://www.sciencedirect.com/science/article/pii/S2405580825000718MyotubesPulsed ultrasoundMAPK signaling pathwayPurine metabolismMitochondrial respiration |
| spellingShingle | Xiaoqi Ma Noriaki Maeshige Atomu Yamaguchi Yunfei Fu Jihao Xing Qingcheng Guo Hao Lin Fuwen Lu Hiroyo Kondo Hidemi Fujino Ultrasound irradiation activates purine metabolism and mitochondrial respiration via the MAPK signaling pathway in myotubes Biochemistry and Biophysics Reports Myotubes Pulsed ultrasound MAPK signaling pathway Purine metabolism Mitochondrial respiration |
| title | Ultrasound irradiation activates purine metabolism and mitochondrial respiration via the MAPK signaling pathway in myotubes |
| title_full | Ultrasound irradiation activates purine metabolism and mitochondrial respiration via the MAPK signaling pathway in myotubes |
| title_fullStr | Ultrasound irradiation activates purine metabolism and mitochondrial respiration via the MAPK signaling pathway in myotubes |
| title_full_unstemmed | Ultrasound irradiation activates purine metabolism and mitochondrial respiration via the MAPK signaling pathway in myotubes |
| title_short | Ultrasound irradiation activates purine metabolism and mitochondrial respiration via the MAPK signaling pathway in myotubes |
| title_sort | ultrasound irradiation activates purine metabolism and mitochondrial respiration via the mapk signaling pathway in myotubes |
| topic | Myotubes Pulsed ultrasound MAPK signaling pathway Purine metabolism Mitochondrial respiration |
| url | http://www.sciencedirect.com/science/article/pii/S2405580825000718 |
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