Biogenic Synthesis of Gold Nanoparticles from Physalis peruviana and Application in Wound Healing

Wound management is key to healing. Poorly managed wounds lead to abnormal biological reactions and complications. Microorganisms, bacteria or fungi, infect such wounds leading to their chronic nature. Gold nanoparticles (Au NPs) show wound healing properties. In addition, ethnobotanical information...

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Main Authors: Stephen Adongo Odongo, Fredrick Oluoch Okumu, Solomon Omwoma Lugasi, Martin Opiyo Onani, Stephen Gaya Agong
Format: Article
Language:English
Published: Wiley 2022-01-01
Series:Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2022/9034840
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author Stephen Adongo Odongo
Fredrick Oluoch Okumu
Solomon Omwoma Lugasi
Martin Opiyo Onani
Stephen Gaya Agong
author_facet Stephen Adongo Odongo
Fredrick Oluoch Okumu
Solomon Omwoma Lugasi
Martin Opiyo Onani
Stephen Gaya Agong
author_sort Stephen Adongo Odongo
collection DOAJ
description Wound management is key to healing. Poorly managed wounds lead to abnormal biological reactions and complications. Microorganisms, bacteria or fungi, infect such wounds leading to their chronic nature. Gold nanoparticles (Au NPs) show wound healing properties. In addition, ethnobotanical information from Siaya County in Kenya shows the leaves of Physalis peruviana L. to be effective in wound management. A combination of Au NPs and leave extracts of Physalis peruviana L. through a one pot biogenic synthesis leads to a new effective wound management substance. The synthesis was done at room temperature 25°C and at 85°C. The UV-visible spectroscopy results show efficient sharper plasmon bands with a blue shift indicating a decrease in λ max compared to red shift which show an increase in λ max. The surface plasmon resonance is a sharper at wavelength of about 540 nm. Dynamic light scattering and zeta potential analysis show that the polydispersity index is high and this is attributed to heterogeneity of chemical components of the plants. Transmission electron microscopy results for Au NPs show similarity in their shapes and sizes with grain size boundaries of between 1 nm and 100 nm. The particles are spherically shaped and crystalline with small lattice due to the small grains. The gold nanoparticles synthesized from Physalis peruviana show antimicrobial activities against gram-positive bacteria and, gram-negative bacteria as well as gram-positive fungus. The inhibition zones for Au NPs of different concentrations vary significantly between concentrations (one-way ANOVA at P<0.05). The highest antibacterial activity is at 100 mM of Au NPs against Escherichia coli, Bacillus subtilis, and Staphylococcus aureus. The inhibition zones for Au NPs at concentration of 100 mM and Physalis peruviana extract vary significantly in all the microbial cells, except for Pseudomonas aeruginosa (one-way ANOVA, F(3,11) = 2.67, P=0.1189). Application of the Au NPs in wound healing is faster than controls. The Au NPs also have good biocompatibility as signs of infection were not present.
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spelling doaj-art-7d60b4ba1985481f93566dcef0ca39642025-02-03T01:19:59ZengWileyJournal of Chemistry2090-90712022-01-01202210.1155/2022/9034840Biogenic Synthesis of Gold Nanoparticles from Physalis peruviana and Application in Wound HealingStephen Adongo Odongo0Fredrick Oluoch Okumu1Solomon Omwoma Lugasi2Martin Opiyo Onani3Stephen Gaya Agong4Department of Physical SciencesDepartment of Physical SciencesDepartment of Physical SciencesDepartment of Physical SciencesDepartment of PlantWound management is key to healing. Poorly managed wounds lead to abnormal biological reactions and complications. Microorganisms, bacteria or fungi, infect such wounds leading to their chronic nature. Gold nanoparticles (Au NPs) show wound healing properties. In addition, ethnobotanical information from Siaya County in Kenya shows the leaves of Physalis peruviana L. to be effective in wound management. A combination of Au NPs and leave extracts of Physalis peruviana L. through a one pot biogenic synthesis leads to a new effective wound management substance. The synthesis was done at room temperature 25°C and at 85°C. The UV-visible spectroscopy results show efficient sharper plasmon bands with a blue shift indicating a decrease in λ max compared to red shift which show an increase in λ max. The surface plasmon resonance is a sharper at wavelength of about 540 nm. Dynamic light scattering and zeta potential analysis show that the polydispersity index is high and this is attributed to heterogeneity of chemical components of the plants. Transmission electron microscopy results for Au NPs show similarity in their shapes and sizes with grain size boundaries of between 1 nm and 100 nm. The particles are spherically shaped and crystalline with small lattice due to the small grains. The gold nanoparticles synthesized from Physalis peruviana show antimicrobial activities against gram-positive bacteria and, gram-negative bacteria as well as gram-positive fungus. The inhibition zones for Au NPs of different concentrations vary significantly between concentrations (one-way ANOVA at P<0.05). The highest antibacterial activity is at 100 mM of Au NPs against Escherichia coli, Bacillus subtilis, and Staphylococcus aureus. The inhibition zones for Au NPs at concentration of 100 mM and Physalis peruviana extract vary significantly in all the microbial cells, except for Pseudomonas aeruginosa (one-way ANOVA, F(3,11) = 2.67, P=0.1189). Application of the Au NPs in wound healing is faster than controls. The Au NPs also have good biocompatibility as signs of infection were not present.http://dx.doi.org/10.1155/2022/9034840
spellingShingle Stephen Adongo Odongo
Fredrick Oluoch Okumu
Solomon Omwoma Lugasi
Martin Opiyo Onani
Stephen Gaya Agong
Biogenic Synthesis of Gold Nanoparticles from Physalis peruviana and Application in Wound Healing
Journal of Chemistry
title Biogenic Synthesis of Gold Nanoparticles from Physalis peruviana and Application in Wound Healing
title_full Biogenic Synthesis of Gold Nanoparticles from Physalis peruviana and Application in Wound Healing
title_fullStr Biogenic Synthesis of Gold Nanoparticles from Physalis peruviana and Application in Wound Healing
title_full_unstemmed Biogenic Synthesis of Gold Nanoparticles from Physalis peruviana and Application in Wound Healing
title_short Biogenic Synthesis of Gold Nanoparticles from Physalis peruviana and Application in Wound Healing
title_sort biogenic synthesis of gold nanoparticles from physalis peruviana and application in wound healing
url http://dx.doi.org/10.1155/2022/9034840
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