LC-MS-Based Global Metabolic Profiles of Alternative Blood Specimens Collected by Microsampling
Blood microsampling (BμS) has recently emerged as an interesting approach in the analysis of endogenous metabolites but also in metabolomics applications. Their non-invasive way of use and the simplified logistics that they offer renders these technologies highly attractive in large-scale studies, e...
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| author | Marlene N. Thaitumu Daniel Marques De Sá e Silva Philippine Louail Johannes Rainer Glykeria Avgerinou Anatoli Petridou Vassilis Mougios Georgios Theodoridis Helen Gika |
| author_facet | Marlene N. Thaitumu Daniel Marques De Sá e Silva Philippine Louail Johannes Rainer Glykeria Avgerinou Anatoli Petridou Vassilis Mougios Georgios Theodoridis Helen Gika |
| author_sort | Marlene N. Thaitumu |
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| description | Blood microsampling (BμS) has recently emerged as an interesting approach in the analysis of endogenous metabolites but also in metabolomics applications. Their non-invasive way of use and the simplified logistics that they offer renders these technologies highly attractive in large-scale studies, especially the novel quantitative microsampling approaches such as VAMs or qDBS. <b>Objectives:</b> Herein, we investigate the potential of BµS devices compared to the conventional plasma samples used in global untargeted mass spectrometry-based metabolomics of blood. <b>Methods:</b> Two novel quantitative devices, namely, Mitra, Capitainer, and the widely used Whatman cards, were selected for comparison with plasma. Venous blood was collected from 10 healthy, overnight-fasted individuals and loaded on the devices; plasma was also collected from the same venous blood. An extraction solvent optimization study was first performed on the three devices before the main study, which compared the global metabolic profiles of the four extracts (three BµS devices and plasma). Analysis was conducted using reverse phase LC-TOF MS in positive mode. <b>Results:</b> BµS devices, especially Mitra and Capitainer, provided equal or even superior information on the metabolic profiling of human blood based on the number and intensity of features and the precision and stability of some annotated metabolites compared to plasma. Despite their rich metabolic profiles, BµS did not capture metabolites associated with biological differentiation of sexes. <b>Conclusions:</b> Overall, our results suggest that a more in-depth investigation of the acquired information is needed for each specific application, as a metabolite-dependent trend was obvious. |
| format | Article |
| id | doaj-art-7d5edbb4036042eb8870b787ac0c04c1 |
| institution | Kabale University |
| issn | 2218-1989 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Metabolites |
| spelling | doaj-art-7d5edbb4036042eb8870b787ac0c04c12025-01-24T13:41:20ZengMDPI AGMetabolites2218-19892025-01-011516210.3390/metabo15010062LC-MS-Based Global Metabolic Profiles of Alternative Blood Specimens Collected by MicrosamplingMarlene N. Thaitumu0Daniel Marques De Sá e Silva1Philippine Louail2Johannes Rainer3Glykeria Avgerinou4Anatoli Petridou5Vassilis Mougios6Georgios Theodoridis7Helen Gika8Department of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceBiomic AUTh, Center for Interdisciplinary Research and Innovation (CIRI-AUTH), Balkan Center, B1.4, 57001 Thessaloniki, GreeceInstitute for Biomedicine, Eurac Research, 39100 Bolzano, ItalyInstitute for Biomedicine, Eurac Research, 39100 Bolzano, ItalySchool of Physical Education & Sport Science at Thessaloniki, Aristotle University of Thessaloniki, 57001 Thessaloniki, GreeceSchool of Physical Education & Sport Science at Thessaloniki, Aristotle University of Thessaloniki, 57001 Thessaloniki, GreeceSchool of Physical Education & Sport Science at Thessaloniki, Aristotle University of Thessaloniki, 57001 Thessaloniki, GreeceBiomic AUTh, Center for Interdisciplinary Research and Innovation (CIRI-AUTH), Balkan Center, B1.4, 57001 Thessaloniki, GreeceDepartment of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceBlood microsampling (BμS) has recently emerged as an interesting approach in the analysis of endogenous metabolites but also in metabolomics applications. Their non-invasive way of use and the simplified logistics that they offer renders these technologies highly attractive in large-scale studies, especially the novel quantitative microsampling approaches such as VAMs or qDBS. <b>Objectives:</b> Herein, we investigate the potential of BµS devices compared to the conventional plasma samples used in global untargeted mass spectrometry-based metabolomics of blood. <b>Methods:</b> Two novel quantitative devices, namely, Mitra, Capitainer, and the widely used Whatman cards, were selected for comparison with plasma. Venous blood was collected from 10 healthy, overnight-fasted individuals and loaded on the devices; plasma was also collected from the same venous blood. An extraction solvent optimization study was first performed on the three devices before the main study, which compared the global metabolic profiles of the four extracts (three BµS devices and plasma). Analysis was conducted using reverse phase LC-TOF MS in positive mode. <b>Results:</b> BµS devices, especially Mitra and Capitainer, provided equal or even superior information on the metabolic profiling of human blood based on the number and intensity of features and the precision and stability of some annotated metabolites compared to plasma. Despite their rich metabolic profiles, BµS did not capture metabolites associated with biological differentiation of sexes. <b>Conclusions:</b> Overall, our results suggest that a more in-depth investigation of the acquired information is needed for each specific application, as a metabolite-dependent trend was obvious.https://www.mdpi.com/2218-1989/15/1/62blood microsampling (BµS)dried blood spot (DBS)quantitative dried blood spots (qDBS)volumetric absorptive microsampling (VAM)liquid chromatography–mass spectrometry (LC-MS)metabolome |
| spellingShingle | Marlene N. Thaitumu Daniel Marques De Sá e Silva Philippine Louail Johannes Rainer Glykeria Avgerinou Anatoli Petridou Vassilis Mougios Georgios Theodoridis Helen Gika LC-MS-Based Global Metabolic Profiles of Alternative Blood Specimens Collected by Microsampling Metabolites blood microsampling (BµS) dried blood spot (DBS) quantitative dried blood spots (qDBS) volumetric absorptive microsampling (VAM) liquid chromatography–mass spectrometry (LC-MS) metabolome |
| title | LC-MS-Based Global Metabolic Profiles of Alternative Blood Specimens Collected by Microsampling |
| title_full | LC-MS-Based Global Metabolic Profiles of Alternative Blood Specimens Collected by Microsampling |
| title_fullStr | LC-MS-Based Global Metabolic Profiles of Alternative Blood Specimens Collected by Microsampling |
| title_full_unstemmed | LC-MS-Based Global Metabolic Profiles of Alternative Blood Specimens Collected by Microsampling |
| title_short | LC-MS-Based Global Metabolic Profiles of Alternative Blood Specimens Collected by Microsampling |
| title_sort | lc ms based global metabolic profiles of alternative blood specimens collected by microsampling |
| topic | blood microsampling (BµS) dried blood spot (DBS) quantitative dried blood spots (qDBS) volumetric absorptive microsampling (VAM) liquid chromatography–mass spectrometry (LC-MS) metabolome |
| url | https://www.mdpi.com/2218-1989/15/1/62 |
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