Biogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific GTPase Rab11B.
Apicomplexan parasites belong to a recently recognised group of protozoa referred to as Alveolata. These protists contain membranous sacs (alveoli) beneath the plasma membrane, termed the Inner Membrane Complex (IMC) in the case of Apicomplexa. During parasite replication the IMC is formed de novo w...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2010-07-01
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| Series: | PLoS Pathogens |
| Online Access: | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1001029&type=printable |
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| author | Carolina Agop-Nersesian Saskia Egarter Gordon Langsley Bernardo J Foth David J P Ferguson Markus Meissner |
| author_facet | Carolina Agop-Nersesian Saskia Egarter Gordon Langsley Bernardo J Foth David J P Ferguson Markus Meissner |
| author_sort | Carolina Agop-Nersesian |
| collection | DOAJ |
| description | Apicomplexan parasites belong to a recently recognised group of protozoa referred to as Alveolata. These protists contain membranous sacs (alveoli) beneath the plasma membrane, termed the Inner Membrane Complex (IMC) in the case of Apicomplexa. During parasite replication the IMC is formed de novo within the mother cell in a process described as internal budding. We hypothesized that an alveolate specific factor is involved in the specific transport of vesicles from the Golgi to the IMC and identified the small GTPase Rab11B as an alveolate specific Rab-GTPase that localises to the growing end of the IMC during replication of Toxoplasma gondii. Conditional interference with Rab11B function leads to a profound defect in IMC biogenesis, indicating that Rab11B is required for the transport of Golgi derived vesicles to the nascent IMC of the daughter cell. Curiously, a block in IMC biogenesis did not affect formation of sub-pellicular microtubules, indicating that IMC biogenesis and formation of sub-pellicular microtubules is not mechanistically linked. We propose a model where Rab11B specifically transports vesicles derived from the Golgi to the immature IMC of the growing daughter parasites. |
| format | Article |
| id | doaj-art-7d5e007436f9431e84cc736d34cfd817 |
| institution | DOAJ |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2010-07-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-7d5e007436f9431e84cc736d34cfd8172025-08-20T03:19:50ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742010-07-0167e100102910.1371/journal.ppat.1001029Biogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific GTPase Rab11B.Carolina Agop-NersesianSaskia EgarterGordon LangsleyBernardo J FothDavid J P FergusonMarkus MeissnerApicomplexan parasites belong to a recently recognised group of protozoa referred to as Alveolata. These protists contain membranous sacs (alveoli) beneath the plasma membrane, termed the Inner Membrane Complex (IMC) in the case of Apicomplexa. During parasite replication the IMC is formed de novo within the mother cell in a process described as internal budding. We hypothesized that an alveolate specific factor is involved in the specific transport of vesicles from the Golgi to the IMC and identified the small GTPase Rab11B as an alveolate specific Rab-GTPase that localises to the growing end of the IMC during replication of Toxoplasma gondii. Conditional interference with Rab11B function leads to a profound defect in IMC biogenesis, indicating that Rab11B is required for the transport of Golgi derived vesicles to the nascent IMC of the daughter cell. Curiously, a block in IMC biogenesis did not affect formation of sub-pellicular microtubules, indicating that IMC biogenesis and formation of sub-pellicular microtubules is not mechanistically linked. We propose a model where Rab11B specifically transports vesicles derived from the Golgi to the immature IMC of the growing daughter parasites.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1001029&type=printable |
| spellingShingle | Carolina Agop-Nersesian Saskia Egarter Gordon Langsley Bernardo J Foth David J P Ferguson Markus Meissner Biogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific GTPase Rab11B. PLoS Pathogens |
| title | Biogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific GTPase Rab11B. |
| title_full | Biogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific GTPase Rab11B. |
| title_fullStr | Biogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific GTPase Rab11B. |
| title_full_unstemmed | Biogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific GTPase Rab11B. |
| title_short | Biogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific GTPase Rab11B. |
| title_sort | biogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific gtpase rab11b |
| url | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1001029&type=printable |
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