Molecular Phylogenetics and Estimation of Evolutionary Divergence and Biogeography of the Family Cordycipitaceae (Ascomycota, Hypocreales)
The Cordycipitaceae family of insecticidal fungi is widely distributed in nature, is the most complex in the order Hypocreales (Ascomycota), with members displaying a diversity of morphological characteristics and insect host ranges. Based on Bayesian evolutionary analysis of five genomic loci(the s...
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2025-01-01
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| author | Huili Pu Jiao Yang Nemat O. Keyhani Lixia Yang Minhai Zheng Chenghao Qiu Yuchen Mao Junya Shang Yongsheng Lin Chengjie Xiong Longbing Lin Pengyu Lai Yanbin Huang Xin Yuan Huiling Liang Longfei Fan Xiaoli Ma Chunjin Qiu Junzhi Qiu |
| author_facet | Huili Pu Jiao Yang Nemat O. Keyhani Lixia Yang Minhai Zheng Chenghao Qiu Yuchen Mao Junya Shang Yongsheng Lin Chengjie Xiong Longbing Lin Pengyu Lai Yanbin Huang Xin Yuan Huiling Liang Longfei Fan Xiaoli Ma Chunjin Qiu Junzhi Qiu |
| author_sort | Huili Pu |
| collection | DOAJ |
| description | The Cordycipitaceae family of insecticidal fungi is widely distributed in nature, is the most complex in the order Hypocreales (Ascomycota), with members displaying a diversity of morphological characteristics and insect host ranges. Based on Bayesian evolutionary analysis of five genomic loci(the small subunit of ribosomal RNA (SSU) gene, the large subunit of ribosomal RNA (LSU) gene, the translation elongation factor 1-α (<i>tef1-α</i>) gene, the largest subunit of RNA polymerase II (<i>rpb1</i>), and the second largest subunit of RNA polymerase II (<i>rpb2</i>), we inferred the divergence times for members of the Cordycipitaceae, improving the internal phylogeny of this fungal family. Molecular clock analyses indicate that the ancestor of <i>Akanthomyces sensu lato</i> occurred in the Paleogene period (34.57 Mya, 95% HPD: 31.41–37.67 Mya), and that most species appeared in the Neogene period. The historical biogeography of <i>Akanthomyces sensu lato</i> was reconstructed using reconstructing ancestral state in phylogenies (RASP) analysis, indicating that it most likely originated in Asia. Combined morphological characterization and phylogenetic analyses were used to identify and taxonomically place five species within Cordycipitaceae. These include the following: (i) two new species, namely <i>Akanthomyces baishanensis</i> sp. nov. and <i>Samsoniella sanmingense</i> sp. nov., (ii) a new record species isolated from infected Lepidopteran host, <i>Blackwellomyces lateris</i>, (iii) a new record species in the genus <i>Niveomyces</i>, with <i>sporothrix</i>-like asexual morphs, namely <i>N. multisynnematus</i>, isolated from dipteran insects (flies), and (iv) a known species of the (hyper-) mycoparasite, <i>Liangia sinensis</i>, isolated from the fungus <i>Ophiocordyceps globiceps</i> (Ophiocordycipitaceae) growing on a dipteran host. Our data provide a significant addition to the diversity, ecology, and evolutionary aspects of the Cordycipitaceae. |
| format | Article |
| id | doaj-art-7d5cfb4bcbb243088806ca819ac7d621 |
| institution | Kabale University |
| issn | 2309-608X |
| language | English |
| publishDate | 2025-01-01 |
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| series | Journal of Fungi |
| spelling | doaj-art-7d5cfb4bcbb243088806ca819ac7d6212025-01-24T13:37:17ZengMDPI AGJournal of Fungi2309-608X2025-01-011112810.3390/jof11010028Molecular Phylogenetics and Estimation of Evolutionary Divergence and Biogeography of the Family Cordycipitaceae (Ascomycota, Hypocreales)Huili Pu0Jiao Yang1Nemat O. Keyhani2Lixia Yang3Minhai Zheng4Chenghao Qiu5Yuchen Mao6Junya Shang7Yongsheng Lin8Chengjie Xiong9Longbing Lin10Pengyu Lai11Yanbin Huang12Xin Yuan13Huiling Liang14Longfei Fan15Xiaoli Ma16Chunjin Qiu17Junzhi Qiu18State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaDepartment of Biological Sciences, University of Illinois, Chicago, IL 60607, USAState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaXiangYa School of Public Health, Central South University, Changsha 410013, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaBureau of Fujian Junzifeng National Nature Reserve, Sanming 365200, ChinaBureau of Fujian Longqishan National Nature Reserve, Sanming 353300, ChinaGuangxi Institute of Botany, Chinese Academy of Sciences, Guilin 541006, ChinaCollege of Plant Protection, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Life Science and Technology, Xinjiang University, Urumqi 830046, ChinaPutian Institute of Agricultural Sciences, Putian 351106, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaThe Cordycipitaceae family of insecticidal fungi is widely distributed in nature, is the most complex in the order Hypocreales (Ascomycota), with members displaying a diversity of morphological characteristics and insect host ranges. Based on Bayesian evolutionary analysis of five genomic loci(the small subunit of ribosomal RNA (SSU) gene, the large subunit of ribosomal RNA (LSU) gene, the translation elongation factor 1-α (<i>tef1-α</i>) gene, the largest subunit of RNA polymerase II (<i>rpb1</i>), and the second largest subunit of RNA polymerase II (<i>rpb2</i>), we inferred the divergence times for members of the Cordycipitaceae, improving the internal phylogeny of this fungal family. Molecular clock analyses indicate that the ancestor of <i>Akanthomyces sensu lato</i> occurred in the Paleogene period (34.57 Mya, 95% HPD: 31.41–37.67 Mya), and that most species appeared in the Neogene period. The historical biogeography of <i>Akanthomyces sensu lato</i> was reconstructed using reconstructing ancestral state in phylogenies (RASP) analysis, indicating that it most likely originated in Asia. Combined morphological characterization and phylogenetic analyses were used to identify and taxonomically place five species within Cordycipitaceae. These include the following: (i) two new species, namely <i>Akanthomyces baishanensis</i> sp. nov. and <i>Samsoniella sanmingense</i> sp. nov., (ii) a new record species isolated from infected Lepidopteran host, <i>Blackwellomyces lateris</i>, (iii) a new record species in the genus <i>Niveomyces</i>, with <i>sporothrix</i>-like asexual morphs, namely <i>N. multisynnematus</i>, isolated from dipteran insects (flies), and (iv) a known species of the (hyper-) mycoparasite, <i>Liangia sinensis</i>, isolated from the fungus <i>Ophiocordyceps globiceps</i> (Ophiocordycipitaceae) growing on a dipteran host. Our data provide a significant addition to the diversity, ecology, and evolutionary aspects of the Cordycipitaceae.https://www.mdpi.com/2309-608X/11/1/28Cordycipitaceaancestral state reconstructionmolecular clockfungal morphologymulti-locus phylogenyfungal taxonomy |
| spellingShingle | Huili Pu Jiao Yang Nemat O. Keyhani Lixia Yang Minhai Zheng Chenghao Qiu Yuchen Mao Junya Shang Yongsheng Lin Chengjie Xiong Longbing Lin Pengyu Lai Yanbin Huang Xin Yuan Huiling Liang Longfei Fan Xiaoli Ma Chunjin Qiu Junzhi Qiu Molecular Phylogenetics and Estimation of Evolutionary Divergence and Biogeography of the Family Cordycipitaceae (Ascomycota, Hypocreales) Journal of Fungi Cordycipitacea ancestral state reconstruction molecular clock fungal morphology multi-locus phylogeny fungal taxonomy |
| title | Molecular Phylogenetics and Estimation of Evolutionary Divergence and Biogeography of the Family Cordycipitaceae (Ascomycota, Hypocreales) |
| title_full | Molecular Phylogenetics and Estimation of Evolutionary Divergence and Biogeography of the Family Cordycipitaceae (Ascomycota, Hypocreales) |
| title_fullStr | Molecular Phylogenetics and Estimation of Evolutionary Divergence and Biogeography of the Family Cordycipitaceae (Ascomycota, Hypocreales) |
| title_full_unstemmed | Molecular Phylogenetics and Estimation of Evolutionary Divergence and Biogeography of the Family Cordycipitaceae (Ascomycota, Hypocreales) |
| title_short | Molecular Phylogenetics and Estimation of Evolutionary Divergence and Biogeography of the Family Cordycipitaceae (Ascomycota, Hypocreales) |
| title_sort | molecular phylogenetics and estimation of evolutionary divergence and biogeography of the family cordycipitaceae ascomycota hypocreales |
| topic | Cordycipitacea ancestral state reconstruction molecular clock fungal morphology multi-locus phylogeny fungal taxonomy |
| url | https://www.mdpi.com/2309-608X/11/1/28 |
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