Gene expression in peripheral blood cells for differentiation of active and latent tuberculosis infection in children and adolescents
The objective: to analyze the expression of certain genes in the blood cells of children and adolescence to differentiate the active and latent phases of tuberculosis infection.Subjects and methods. Peripheral blood samples collected in 36 pediatric patients with latent tuberculosis infection and 24...
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| Format: | Article |
| Language: | Russian |
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New Terra Publishing House
2020-01-01
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| Series: | Туберкулез и болезни лёгких |
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| Online Access: | https://www.tibl-journal.com/jour/article/view/1367 |
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| author | A. A. Elov M. A. Vladimirskiy S. V. Smerdin O. I. Elufimova |
| author_facet | A. A. Elov M. A. Vladimirskiy S. V. Smerdin O. I. Elufimova |
| author_sort | A. A. Elov |
| collection | DOAJ |
| description | The objective: to analyze the expression of certain genes in the blood cells of children and adolescence to differentiate the active and latent phases of tuberculosis infection.Subjects and methods. Peripheral blood samples collected in 36 pediatric patients with latent tuberculosis infection and 24 patients aged 1 to 16 years undergoing in-patient treatment for pulmonary tuberculosis were tested. A modified method for isolating messenger RNA and reverse transcriptional polymerase chain reaction was used to identify the transcription of six genes selected for analysis.Results. In a comparative study of the expression values of six promising genes in blood cells in the study of two groups of children and adolescents with latent and active tuberculosis infection, it was found that the most differentiating feature for determining active tuberculosis infection was a significantly higher level of expression of PDCD1 gene encoding PD1 lymphocyte receptor. At the same time, the sensitivity to detect the active infection was found to be 95.8%, specificity – 94.4%, the accuracy of the positive prognosis of active tuberculosis infection was 93.3%. |
| format | Article |
| id | doaj-art-7d55d602f83c441dafb950af1ee73166 |
| institution | OA Journals |
| issn | 2075-1230 2542-1506 |
| language | Russian |
| publishDate | 2020-01-01 |
| publisher | New Terra Publishing House |
| record_format | Article |
| series | Туберкулез и болезни лёгких |
| spelling | doaj-art-7d55d602f83c441dafb950af1ee731662025-08-20T02:05:48ZrusNew Terra Publishing HouseТуберкулез и болезни лёгких2075-12302542-15062020-01-019712283210.21292/2075-1230-2019-97-12-28-321367Gene expression in peripheral blood cells for differentiation of active and latent tuberculosis infection in children and adolescentsA. A. Elov0M. A. Vladimirskiy1S. V. Smerdin2O. I. Elufimova3National Medical Research Center of Phthisiopulmonology and Infectious DiseasesNational Medical Research Center of Phthisiopulmonology and Infectious DiseasesMoscow Regional TB DispensaryMoscow Regional TB DispensaryThe objective: to analyze the expression of certain genes in the blood cells of children and adolescence to differentiate the active and latent phases of tuberculosis infection.Subjects and methods. Peripheral blood samples collected in 36 pediatric patients with latent tuberculosis infection and 24 patients aged 1 to 16 years undergoing in-patient treatment for pulmonary tuberculosis were tested. A modified method for isolating messenger RNA and reverse transcriptional polymerase chain reaction was used to identify the transcription of six genes selected for analysis.Results. In a comparative study of the expression values of six promising genes in blood cells in the study of two groups of children and adolescents with latent and active tuberculosis infection, it was found that the most differentiating feature for determining active tuberculosis infection was a significantly higher level of expression of PDCD1 gene encoding PD1 lymphocyte receptor. At the same time, the sensitivity to detect the active infection was found to be 95.8%, specificity – 94.4%, the accuracy of the positive prognosis of active tuberculosis infection was 93.3%.https://www.tibl-journal.com/jour/article/view/1367latent and active tuberculosis infectiongene expressionmessenger rnapd1 receptor |
| spellingShingle | A. A. Elov M. A. Vladimirskiy S. V. Smerdin O. I. Elufimova Gene expression in peripheral blood cells for differentiation of active and latent tuberculosis infection in children and adolescents Туберкулез и болезни лёгких latent and active tuberculosis infection gene expression messenger rna pd1 receptor |
| title | Gene expression in peripheral blood cells for differentiation of active and latent tuberculosis infection in children and adolescents |
| title_full | Gene expression in peripheral blood cells for differentiation of active and latent tuberculosis infection in children and adolescents |
| title_fullStr | Gene expression in peripheral blood cells for differentiation of active and latent tuberculosis infection in children and adolescents |
| title_full_unstemmed | Gene expression in peripheral blood cells for differentiation of active and latent tuberculosis infection in children and adolescents |
| title_short | Gene expression in peripheral blood cells for differentiation of active and latent tuberculosis infection in children and adolescents |
| title_sort | gene expression in peripheral blood cells for differentiation of active and latent tuberculosis infection in children and adolescents |
| topic | latent and active tuberculosis infection gene expression messenger rna pd1 receptor |
| url | https://www.tibl-journal.com/jour/article/view/1367 |
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