Proteomic Analysis of <i>Bifidobacterium animalis</i> AR668 and AR668-R1 Under Aerobic Culture
<i>Bifidobacterium animalis</i> is a widely used probiotic with significant health benefits, but its application is limited by oxygen sensitivity. Our laboratory previously developed an oxygen-tolerant <i>B. animalis</i> AR668-R1 using adaptive laboratory evolution under aero...
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MDPI AG
2025-05-01
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| author | Yaping Liu Xiaoxiao Zhao Miao Yang Xin Song Guangqiang Wang Yongjun Xia Liang Zhao Zhiqiang Xiong Lianzhong Ai |
| author_facet | Yaping Liu Xiaoxiao Zhao Miao Yang Xin Song Guangqiang Wang Yongjun Xia Liang Zhao Zhiqiang Xiong Lianzhong Ai |
| author_sort | Yaping Liu |
| collection | DOAJ |
| description | <i>Bifidobacterium animalis</i> is a widely used probiotic with significant health benefits, but its application is limited by oxygen sensitivity. Our laboratory previously developed an oxygen-tolerant <i>B. animalis</i> AR668-R1 using adaptive laboratory evolution under aerobic culture, but the molecular mechanism remains unclear. In this work, compared to the wild-type parental strain <i>B. animalis</i> AR668, 212 upregulated and 390 downregulated proteins were identified in AR668-R1 under aerobic conditions through comparative proteomic analysis. Enrichment analysis of the differentially expressed proteins between AR668 and AR668-R1 identified the potential oxygen-tolerant related pathways, including the translation process, transmembrane transport system, and carbohydrate metabolism. Furthermore, five potential oxygen-tolerance proteins (DapE, Mth2, MutT, Eno, and MsrAB) were validated by RT-qPCR that may contribute to the aerobic growth of AR668-R1. Through gene overexpression validation, Mth2 (7,8-dihydro-8-oxoguanine triphosphatase) was found to enhance the growth of AR668-R1 by 19.8% compared to the empty plasmid control under aerobic conditions. Our finding provides valuable insights into the oxygen-tolerant mechanisms of <i>B. animalis</i> at the protein level. |
| format | Article |
| id | doaj-art-7d55b2a57bd24e8bad2f66d2ec8757c7 |
| institution | DOAJ |
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| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-7d55b2a57bd24e8bad2f66d2ec8757c72025-08-20T03:14:31ZengMDPI AGFoods2304-81582025-05-011410176610.3390/foods14101766Proteomic Analysis of <i>Bifidobacterium animalis</i> AR668 and AR668-R1 Under Aerobic CultureYaping Liu0Xiaoxiao Zhao1Miao Yang2Xin Song3Guangqiang Wang4Yongjun Xia5Liang Zhao6Zhiqiang Xiong7Lianzhong Ai8Shanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaShanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China<i>Bifidobacterium animalis</i> is a widely used probiotic with significant health benefits, but its application is limited by oxygen sensitivity. Our laboratory previously developed an oxygen-tolerant <i>B. animalis</i> AR668-R1 using adaptive laboratory evolution under aerobic culture, but the molecular mechanism remains unclear. In this work, compared to the wild-type parental strain <i>B. animalis</i> AR668, 212 upregulated and 390 downregulated proteins were identified in AR668-R1 under aerobic conditions through comparative proteomic analysis. Enrichment analysis of the differentially expressed proteins between AR668 and AR668-R1 identified the potential oxygen-tolerant related pathways, including the translation process, transmembrane transport system, and carbohydrate metabolism. Furthermore, five potential oxygen-tolerance proteins (DapE, Mth2, MutT, Eno, and MsrAB) were validated by RT-qPCR that may contribute to the aerobic growth of AR668-R1. Through gene overexpression validation, Mth2 (7,8-dihydro-8-oxoguanine triphosphatase) was found to enhance the growth of AR668-R1 by 19.8% compared to the empty plasmid control under aerobic conditions. Our finding provides valuable insights into the oxygen-tolerant mechanisms of <i>B. animalis</i> at the protein level.https://www.mdpi.com/2304-8158/14/10/1766<i>Bifidobacterium animalis</i>oxygen-tolerantproteomic analysis |
| spellingShingle | Yaping Liu Xiaoxiao Zhao Miao Yang Xin Song Guangqiang Wang Yongjun Xia Liang Zhao Zhiqiang Xiong Lianzhong Ai Proteomic Analysis of <i>Bifidobacterium animalis</i> AR668 and AR668-R1 Under Aerobic Culture Foods <i>Bifidobacterium animalis</i> oxygen-tolerant proteomic analysis |
| title | Proteomic Analysis of <i>Bifidobacterium animalis</i> AR668 and AR668-R1 Under Aerobic Culture |
| title_full | Proteomic Analysis of <i>Bifidobacterium animalis</i> AR668 and AR668-R1 Under Aerobic Culture |
| title_fullStr | Proteomic Analysis of <i>Bifidobacterium animalis</i> AR668 and AR668-R1 Under Aerobic Culture |
| title_full_unstemmed | Proteomic Analysis of <i>Bifidobacterium animalis</i> AR668 and AR668-R1 Under Aerobic Culture |
| title_short | Proteomic Analysis of <i>Bifidobacterium animalis</i> AR668 and AR668-R1 Under Aerobic Culture |
| title_sort | proteomic analysis of i bifidobacterium animalis i ar668 and ar668 r1 under aerobic culture |
| topic | <i>Bifidobacterium animalis</i> oxygen-tolerant proteomic analysis |
| url | https://www.mdpi.com/2304-8158/14/10/1766 |
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