Uptake of HLA Alloantigens via CD89 and CD206 Does Not Enhance Antigen Presentation by Indirect Allorecognition
In organ transplantation, alloantigens are taken up by antigen presenting cells and presented via the indirect pathway to T-cells which in turn can induce allograft rejection. Monitoring of these T-cells is of major importance; however no reliable assay is available to routinely monitor indirect all...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/4215684 |
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| author | Eytan Breman Jurjen M. Ruben Kees L. Franken Mirjam H. M. Heemskerk Dave L. Roelen Frans H. Claas Cees van Kooten |
| author_facet | Eytan Breman Jurjen M. Ruben Kees L. Franken Mirjam H. M. Heemskerk Dave L. Roelen Frans H. Claas Cees van Kooten |
| author_sort | Eytan Breman |
| collection | DOAJ |
| description | In organ transplantation, alloantigens are taken up by antigen presenting cells and presented via the indirect pathway to T-cells which in turn can induce allograft rejection. Monitoring of these T-cells is of major importance; however no reliable assay is available to routinely monitor indirect allorecognition. Recently we showed that HLA monomers can be successfully used to monitor indirect allorecognition. Targeting antigens to endocytic receptors on antigen presenting cells may further enhance the presentation of antigens via HLA class II and improve the efficiency of this assay. In the current study we explored targeting of HLA monomers to either CD89 expressing monocytes or mannose receptor expressing dendritic cells. Monomer-antibody complexes were generated using biotin-labeled monomers and avidin labeling of the antibodies. We demonstrate that targeting the complexes to these receptors resulted in a dose-dependent HLA class II mediated presentation to a T-cell clone. The immune-complexes were efficiently taken up and presented to T-cells. However, the level of T-cell reactivity was similar to that when only exogenous antigen was added. We conclude that HLA-A2 monomers targeted for presentation through CD89 on monocytes or mannose receptor on dendritic cells lead to proper antigen presentation but do not enhance indirect allorecognition via HLA-DR. |
| format | Article |
| id | doaj-art-7d51bfd1bb4d4304ab38afd41f3d8a15 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-7d51bfd1bb4d4304ab38afd41f3d8a152025-08-20T03:35:19ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/42156844215684Uptake of HLA Alloantigens via CD89 and CD206 Does Not Enhance Antigen Presentation by Indirect AllorecognitionEytan Breman0Jurjen M. Ruben1Kees L. Franken2Mirjam H. M. Heemskerk3Dave L. Roelen4Frans H. Claas5Cees van Kooten6Department of Nephrology, Leiden University Medical Center (LUMC), 2333 ZA Leiden, NetherlandsDepartment of Nephrology, Leiden University Medical Center (LUMC), 2333 ZA Leiden, NetherlandsDepartment of Immunohematology and Blood Transfusion, LUMC, 2333 ZA Leiden, NetherlandsDepartment of Hematology, LUMC, 2333 ZA Leiden, NetherlandsDepartment of Immunohematology and Blood Transfusion, LUMC, 2333 ZA Leiden, NetherlandsDepartment of Immunohematology and Blood Transfusion, LUMC, 2333 ZA Leiden, NetherlandsDepartment of Nephrology, Leiden University Medical Center (LUMC), 2333 ZA Leiden, NetherlandsIn organ transplantation, alloantigens are taken up by antigen presenting cells and presented via the indirect pathway to T-cells which in turn can induce allograft rejection. Monitoring of these T-cells is of major importance; however no reliable assay is available to routinely monitor indirect allorecognition. Recently we showed that HLA monomers can be successfully used to monitor indirect allorecognition. Targeting antigens to endocytic receptors on antigen presenting cells may further enhance the presentation of antigens via HLA class II and improve the efficiency of this assay. In the current study we explored targeting of HLA monomers to either CD89 expressing monocytes or mannose receptor expressing dendritic cells. Monomer-antibody complexes were generated using biotin-labeled monomers and avidin labeling of the antibodies. We demonstrate that targeting the complexes to these receptors resulted in a dose-dependent HLA class II mediated presentation to a T-cell clone. The immune-complexes were efficiently taken up and presented to T-cells. However, the level of T-cell reactivity was similar to that when only exogenous antigen was added. We conclude that HLA-A2 monomers targeted for presentation through CD89 on monocytes or mannose receptor on dendritic cells lead to proper antigen presentation but do not enhance indirect allorecognition via HLA-DR.http://dx.doi.org/10.1155/2016/4215684 |
| spellingShingle | Eytan Breman Jurjen M. Ruben Kees L. Franken Mirjam H. M. Heemskerk Dave L. Roelen Frans H. Claas Cees van Kooten Uptake of HLA Alloantigens via CD89 and CD206 Does Not Enhance Antigen Presentation by Indirect Allorecognition Journal of Immunology Research |
| title | Uptake of HLA Alloantigens via CD89 and CD206 Does Not Enhance Antigen Presentation by Indirect Allorecognition |
| title_full | Uptake of HLA Alloantigens via CD89 and CD206 Does Not Enhance Antigen Presentation by Indirect Allorecognition |
| title_fullStr | Uptake of HLA Alloantigens via CD89 and CD206 Does Not Enhance Antigen Presentation by Indirect Allorecognition |
| title_full_unstemmed | Uptake of HLA Alloantigens via CD89 and CD206 Does Not Enhance Antigen Presentation by Indirect Allorecognition |
| title_short | Uptake of HLA Alloantigens via CD89 and CD206 Does Not Enhance Antigen Presentation by Indirect Allorecognition |
| title_sort | uptake of hla alloantigens via cd89 and cd206 does not enhance antigen presentation by indirect allorecognition |
| url | http://dx.doi.org/10.1155/2016/4215684 |
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