Hepatotoxicity induced by subacute exposure of Mancozeb and Arsenic in Wistar rats: Mitigating effect of quercetin and catechin
Background: Widespread use of Mancozeb (MZ) fungicide in endemic areas having high levels of arsenic (As+3) in the groundwater is likely to cause toxicosis in humans and animals. Therefore, the present study aimed to determine the hepatotoxic effect of MZ and As+3 interaction in Wistar rats and miti...
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Elsevier
2024-12-01
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| Series: | Journal of Trace Elements and Minerals |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773050624000843 |
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| author | Rasia Yousuf Pawan Kumar Verma Priyanka Sharma Shilpa Sood Nrip K. Pankaj Zuhaib F. Bhat |
| author_facet | Rasia Yousuf Pawan Kumar Verma Priyanka Sharma Shilpa Sood Nrip K. Pankaj Zuhaib F. Bhat |
| author_sort | Rasia Yousuf |
| collection | DOAJ |
| description | Background: Widespread use of Mancozeb (MZ) fungicide in endemic areas having high levels of arsenic (As+3) in the groundwater is likely to cause toxicosis in humans and animals. Therefore, the present study aimed to determine the hepatotoxic effect of MZ and As+3 interaction in Wistar rats and mitigating potential of quercetin and catechin on such toxicity. Methods: Sixty adult rats were randomly divided into 10 groups of 6 animals each. Group I served as control and group II was exposed to MZ (800 mg kg-1, PO). Groups III, IV, and V were provided drinking water containing As+3 at the rate of 10, 50 and 100 µg L-1, respectively. Groups VI, VII, and VIII were given drinking water containing As+3 at 10, 50 and 100 µg L-1, respectively, along with MZ. Groups IX and X received drinking water contaminated with 100 µg L-1, As+3 and MZ along with quercetin or catechin (50 mg kg-1 each), respectively. Results: Significant (p <0.05) alterations in hepatic biomarkers in blood, liver antioxidant status and hepatic histoarchitecture were observed in animals treated with individual toxicants in a dose-dependent manner, however, such toxicity-induced changes were more severe in co-exposed rats. The administration of quercetin or catechin significantly mitigated the dual toxicant administration-driven changes in biochemical markers of hepatic damage, hepatic antioxidant profile and liver histomorphology. Conclusions: Both quercetin and catechin proved beneficial in reducing toxicant-induced oxidation-mediated hepatic damage as demonstrated by alleviation in altered levels of determinants of hepatic biochemical and oxidative stress parameters in addition to restoration of toxicant-induced histological alterations of hepatic tissue. |
| format | Article |
| id | doaj-art-7d42d5f0a8dc47cbaf7c11a0ca2bce8b |
| institution | OA Journals |
| issn | 2773-0506 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Trace Elements and Minerals |
| spelling | doaj-art-7d42d5f0a8dc47cbaf7c11a0ca2bce8b2025-08-20T02:20:38ZengElsevierJournal of Trace Elements and Minerals2773-05062024-12-011010019910.1016/j.jtemin.2024.100199Hepatotoxicity induced by subacute exposure of Mancozeb and Arsenic in Wistar rats: Mitigating effect of quercetin and catechinRasia Yousuf0Pawan Kumar Verma1Priyanka Sharma2Shilpa Sood3Nrip K. Pankaj4Zuhaib F. Bhat5Division of Veterinary Pharmacology and Toxicology, F.V.Sc. & A.H., Jammu, J&K, 181102, IndiaDivision of Veterinary Pharmacology and Toxicology, F.V.Sc. & A.H., Jammu, J&K, 181102, India; Corresponding authors.Division of Veterinary Pharmacology and Toxicology, F.V.Sc. & A.H., Jammu, J&K, 181102, IndiaDivision of Veterinary Pathology, F.V.Sc. & A.H., Jammu, J&K, 181102, IndiaDivision of Veterinary Pharmacology and Toxicology, F.V.Sc. & A.H., Jammu, J&K, 181102, IndiaDivision of Livestock Products Technology, SKUAST-Jammu, India, 181102; Corresponding authors.Background: Widespread use of Mancozeb (MZ) fungicide in endemic areas having high levels of arsenic (As+3) in the groundwater is likely to cause toxicosis in humans and animals. Therefore, the present study aimed to determine the hepatotoxic effect of MZ and As+3 interaction in Wistar rats and mitigating potential of quercetin and catechin on such toxicity. Methods: Sixty adult rats were randomly divided into 10 groups of 6 animals each. Group I served as control and group II was exposed to MZ (800 mg kg-1, PO). Groups III, IV, and V were provided drinking water containing As+3 at the rate of 10, 50 and 100 µg L-1, respectively. Groups VI, VII, and VIII were given drinking water containing As+3 at 10, 50 and 100 µg L-1, respectively, along with MZ. Groups IX and X received drinking water contaminated with 100 µg L-1, As+3 and MZ along with quercetin or catechin (50 mg kg-1 each), respectively. Results: Significant (p <0.05) alterations in hepatic biomarkers in blood, liver antioxidant status and hepatic histoarchitecture were observed in animals treated with individual toxicants in a dose-dependent manner, however, such toxicity-induced changes were more severe in co-exposed rats. The administration of quercetin or catechin significantly mitigated the dual toxicant administration-driven changes in biochemical markers of hepatic damage, hepatic antioxidant profile and liver histomorphology. Conclusions: Both quercetin and catechin proved beneficial in reducing toxicant-induced oxidation-mediated hepatic damage as demonstrated by alleviation in altered levels of determinants of hepatic biochemical and oxidative stress parameters in addition to restoration of toxicant-induced histological alterations of hepatic tissue.http://www.sciencedirect.com/science/article/pii/S2773050624000843MancozebArsenicQuercetinCatechinOxidative damageHepatotoxicity |
| spellingShingle | Rasia Yousuf Pawan Kumar Verma Priyanka Sharma Shilpa Sood Nrip K. Pankaj Zuhaib F. Bhat Hepatotoxicity induced by subacute exposure of Mancozeb and Arsenic in Wistar rats: Mitigating effect of quercetin and catechin Journal of Trace Elements and Minerals Mancozeb Arsenic Quercetin Catechin Oxidative damage Hepatotoxicity |
| title | Hepatotoxicity induced by subacute exposure of Mancozeb and Arsenic in Wistar rats: Mitigating effect of quercetin and catechin |
| title_full | Hepatotoxicity induced by subacute exposure of Mancozeb and Arsenic in Wistar rats: Mitigating effect of quercetin and catechin |
| title_fullStr | Hepatotoxicity induced by subacute exposure of Mancozeb and Arsenic in Wistar rats: Mitigating effect of quercetin and catechin |
| title_full_unstemmed | Hepatotoxicity induced by subacute exposure of Mancozeb and Arsenic in Wistar rats: Mitigating effect of quercetin and catechin |
| title_short | Hepatotoxicity induced by subacute exposure of Mancozeb and Arsenic in Wistar rats: Mitigating effect of quercetin and catechin |
| title_sort | hepatotoxicity induced by subacute exposure of mancozeb and arsenic in wistar rats mitigating effect of quercetin and catechin |
| topic | Mancozeb Arsenic Quercetin Catechin Oxidative damage Hepatotoxicity |
| url | http://www.sciencedirect.com/science/article/pii/S2773050624000843 |
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