<i>Marantodes pumilum</i> var. <i>alata</i> Enhances Fracture Healing Through Gene Regulation in a Postmenopausal Rat Model
<b>Background:</b> <i>Marantodes pumilum</i> var. <i>alata</i> (<i>MPva</i>) has been reported to promote fracture repair. This study investigates the role of <i>MPva</i> leaf extract on biochemical markers and bone-repair genes in a postme...
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MDPI AG
2025-05-01
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| author | Tijjani Rabiu Giaze Norazlina Mohamed Syed Alhafiz Syed Hashim Ahmad Nazrun Shuid Ima Nirwana Soelaiman Norliza Muhammad Fadhlullah Zuhair Jafar Sidik Jamia Azdina Jamal |
| author_facet | Tijjani Rabiu Giaze Norazlina Mohamed Syed Alhafiz Syed Hashim Ahmad Nazrun Shuid Ima Nirwana Soelaiman Norliza Muhammad Fadhlullah Zuhair Jafar Sidik Jamia Azdina Jamal |
| author_sort | Tijjani Rabiu Giaze |
| collection | DOAJ |
| description | <b>Background:</b> <i>Marantodes pumilum</i> var. <i>alata</i> (<i>MPva</i>) has been reported to promote fracture repair. This study investigates the role of <i>MPva</i> leaf extract on biochemical markers and bone-repair genes in a postmenopausal rat model to understand its fracture-healing properties. <b>Methods</b>: Thirty female Sprague Dawley rats were grouped into sham-operated (Sham), ovariectomized control (OVXC), estrogen treatment (ERT), and plant treatment (MPv20 and MPv100) groups. After ovariectomy, the right tibiae of rats were fractured. The ERT group was treated with 64.5 μg/kg/day of estrogen, while the MPv20 and MPv100 groups received 20 and 100 mg/kg/day doses of <i>MPva</i> leaf extract, respectively, for 8 weeks. Sham and OVXC acted as untreated controls. Blood samples collected before and after treatment were assayed for pro-inflammatory cytokines (IL-6 and TNF-α), while bone samples were assayed for bone-turnover markers: osteocalcin and pyridinoline, oxidative-status markers (GPx, SOD, and MDA), and bone-repair genes (<i>Bglap</i>, <i>Spp1</i>, <i>Dkk1</i>, <i>Igf1</i>, <i>Tnfsf11</i>, and <i>Fgf23</i>). <b>Results</b>: IL-6, GPx, and SOD levels were significantly increased in both MPv groups (<i>p</i> < 0.05). <i>IGF1</i> was significantly upregulated in both MPv groups, while <i>Tnfsf11</i> was downregulated in the MPv20 group (<i>p</i> < 0.05). <b>Conclusions</b>: <i>MPva</i> leaf extract may promote bone repair by stimulating pro-inflammatory and antioxidant responses, which are associated with its regulation of <i>Igf1</i> and <i>Tnfsf11 genes</i>. |
| format | Article |
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| spelling | doaj-art-7d41a912807d4e02840b870aced0c2ad2025-08-20T02:33:48ZengMDPI AGPharmaceuticals1424-82472025-05-0118573610.3390/ph18050736<i>Marantodes pumilum</i> var. <i>alata</i> Enhances Fracture Healing Through Gene Regulation in a Postmenopausal Rat ModelTijjani Rabiu Giaze0Norazlina Mohamed1Syed Alhafiz Syed Hashim2Ahmad Nazrun Shuid3Ima Nirwana Soelaiman4Norliza Muhammad5Fadhlullah Zuhair Jafar Sidik6Jamia Azdina Jamal7Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur 56000, Selangor, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur 56000, Selangor, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur 56000, Selangor, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Teknologi Mara, Sungai Buloh 47000, Selangor, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur 56000, Selangor, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur 56000, Selangor, MalaysiaFaculty of Pharmacy, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur 56000, Selangor, MalaysiaFaculty of Pharmacy, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur 56000, Selangor, Malaysia<b>Background:</b> <i>Marantodes pumilum</i> var. <i>alata</i> (<i>MPva</i>) has been reported to promote fracture repair. This study investigates the role of <i>MPva</i> leaf extract on biochemical markers and bone-repair genes in a postmenopausal rat model to understand its fracture-healing properties. <b>Methods</b>: Thirty female Sprague Dawley rats were grouped into sham-operated (Sham), ovariectomized control (OVXC), estrogen treatment (ERT), and plant treatment (MPv20 and MPv100) groups. After ovariectomy, the right tibiae of rats were fractured. The ERT group was treated with 64.5 μg/kg/day of estrogen, while the MPv20 and MPv100 groups received 20 and 100 mg/kg/day doses of <i>MPva</i> leaf extract, respectively, for 8 weeks. Sham and OVXC acted as untreated controls. Blood samples collected before and after treatment were assayed for pro-inflammatory cytokines (IL-6 and TNF-α), while bone samples were assayed for bone-turnover markers: osteocalcin and pyridinoline, oxidative-status markers (GPx, SOD, and MDA), and bone-repair genes (<i>Bglap</i>, <i>Spp1</i>, <i>Dkk1</i>, <i>Igf1</i>, <i>Tnfsf11</i>, and <i>Fgf23</i>). <b>Results</b>: IL-6, GPx, and SOD levels were significantly increased in both MPv groups (<i>p</i> < 0.05). <i>IGF1</i> was significantly upregulated in both MPv groups, while <i>Tnfsf11</i> was downregulated in the MPv20 group (<i>p</i> < 0.05). <b>Conclusions</b>: <i>MPva</i> leaf extract may promote bone repair by stimulating pro-inflammatory and antioxidant responses, which are associated with its regulation of <i>Igf1</i> and <i>Tnfsf11 genes</i>.https://www.mdpi.com/1424-8247/18/5/736fracturegene expression<i>Marantodes pumilum</i>osteoporosisovariectomized rats |
| spellingShingle | Tijjani Rabiu Giaze Norazlina Mohamed Syed Alhafiz Syed Hashim Ahmad Nazrun Shuid Ima Nirwana Soelaiman Norliza Muhammad Fadhlullah Zuhair Jafar Sidik Jamia Azdina Jamal <i>Marantodes pumilum</i> var. <i>alata</i> Enhances Fracture Healing Through Gene Regulation in a Postmenopausal Rat Model Pharmaceuticals fracture gene expression <i>Marantodes pumilum</i> osteoporosis ovariectomized rats |
| title | <i>Marantodes pumilum</i> var. <i>alata</i> Enhances Fracture Healing Through Gene Regulation in a Postmenopausal Rat Model |
| title_full | <i>Marantodes pumilum</i> var. <i>alata</i> Enhances Fracture Healing Through Gene Regulation in a Postmenopausal Rat Model |
| title_fullStr | <i>Marantodes pumilum</i> var. <i>alata</i> Enhances Fracture Healing Through Gene Regulation in a Postmenopausal Rat Model |
| title_full_unstemmed | <i>Marantodes pumilum</i> var. <i>alata</i> Enhances Fracture Healing Through Gene Regulation in a Postmenopausal Rat Model |
| title_short | <i>Marantodes pumilum</i> var. <i>alata</i> Enhances Fracture Healing Through Gene Regulation in a Postmenopausal Rat Model |
| title_sort | i marantodes pumilum i var i alata i enhances fracture healing through gene regulation in a postmenopausal rat model |
| topic | fracture gene expression <i>Marantodes pumilum</i> osteoporosis ovariectomized rats |
| url | https://www.mdpi.com/1424-8247/18/5/736 |
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