Nutrient Induced Type 2 and Chemical Induced Type 1 Experimental Diabetes Differently Modulate Gastric GLP-1 Receptor Expression
T2DM patients demonstrate reduced GLP-1 receptor (GLP-1R) expression in their gastric glands. Whether induced T2DM and T1DM differently affect the gastric GLP-1R expression is not known. This study assessed extrapancreatic GLP-1R system in glandular stomach of rodents with different types of experim...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2015/561353 |
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| author | Olga Bloch Efrat Broide Gilad Ben-Yehudah Dror Cantrell Haim Shirin Micha J. Rapoport |
| author_facet | Olga Bloch Efrat Broide Gilad Ben-Yehudah Dror Cantrell Haim Shirin Micha J. Rapoport |
| author_sort | Olga Bloch |
| collection | DOAJ |
| description | T2DM patients demonstrate reduced GLP-1 receptor (GLP-1R) expression in their gastric glands. Whether induced T2DM and T1DM differently affect the gastric GLP-1R expression is not known. This study assessed extrapancreatic GLP-1R system in glandular stomach of rodents with different types of experimental diabetes. T2DM and T1DM were induced in Psammomys obesus (PO) by high-energy (HE) diet and by streptozotocin (STZ) in Sprague Dawly (SD) rats, respectively. GLP-1R expression was determined in glandular stomach by RT PCR and immunohistomorphological analysis. The mRNA expression and cellular association of the GLP-1R in principal glands were similar in control PO and SD rats. However, nutrient and chemical induced diabetes resulted in opposite alterations of glandular GLP-1R expression. Diabetic PO demonstrated increased GLP-1R mRNA expression, intensity of cellular GLP-1R immunostaining, and frequency of GLP-1R positive cells in the neck area of principal glands compared with controls. In contrast, SD diabetic rats demonstrated decreased GLP-1 mRNA, cellular GLP-1R immunoreactivity, and frequency of GLP-1R immunoreactive cells in the neck area compared with controls. In conclusion, nutrient and chemical induced experimental diabetes result in distinct opposite alterations of GLP-1R expression in glandular stomach. These results suggest that induced T1DM and T2DM may differently modulate GLP-1R system in enteropancreatic axis. |
| format | Article |
| id | doaj-art-7d3376fbe8da4617a34755c85230ac64 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-7d3376fbe8da4617a34755c85230ac642025-02-03T01:32:11ZengWileyJournal of Diabetes Research2314-67452314-67532015-01-01201510.1155/2015/561353561353Nutrient Induced Type 2 and Chemical Induced Type 1 Experimental Diabetes Differently Modulate Gastric GLP-1 Receptor ExpressionOlga Bloch0Efrat Broide1Gilad Ben-Yehudah2Dror Cantrell3Haim Shirin4Micha J. Rapoport5Diabetes and Immunology Research Laboratory, Assaf Harofeh Medical Center Affiliated to the Sackler Faculty of Medicine, Tel-Aviv University, 70300 Zerifin, IsraelInstitute of Gastroenterology, Assaf Harofeh Medical Center Affiliated to the Sackler Faculty of Medicine, Tel-Aviv University, 70300 Zerifin, IsraelInstitute of Gastroenterology, Assaf Harofeh Medical Center Affiliated to the Sackler Faculty of Medicine, Tel-Aviv University, 70300 Zerifin, IsraelDepartment of Internal Medicine “C”, Assaf Harofeh Medical Center Affiliated to the Sackler Faculty of Medicine, Tel-Aviv University, 70300 Zerifin, IsraelInstitute of Gastroenterology, Assaf Harofeh Medical Center Affiliated to the Sackler Faculty of Medicine, Tel-Aviv University, 70300 Zerifin, IsraelDiabetes and Immunology Research Laboratory, Assaf Harofeh Medical Center Affiliated to the Sackler Faculty of Medicine, Tel-Aviv University, 70300 Zerifin, IsraelT2DM patients demonstrate reduced GLP-1 receptor (GLP-1R) expression in their gastric glands. Whether induced T2DM and T1DM differently affect the gastric GLP-1R expression is not known. This study assessed extrapancreatic GLP-1R system in glandular stomach of rodents with different types of experimental diabetes. T2DM and T1DM were induced in Psammomys obesus (PO) by high-energy (HE) diet and by streptozotocin (STZ) in Sprague Dawly (SD) rats, respectively. GLP-1R expression was determined in glandular stomach by RT PCR and immunohistomorphological analysis. The mRNA expression and cellular association of the GLP-1R in principal glands were similar in control PO and SD rats. However, nutrient and chemical induced diabetes resulted in opposite alterations of glandular GLP-1R expression. Diabetic PO demonstrated increased GLP-1R mRNA expression, intensity of cellular GLP-1R immunostaining, and frequency of GLP-1R positive cells in the neck area of principal glands compared with controls. In contrast, SD diabetic rats demonstrated decreased GLP-1 mRNA, cellular GLP-1R immunoreactivity, and frequency of GLP-1R immunoreactive cells in the neck area compared with controls. In conclusion, nutrient and chemical induced experimental diabetes result in distinct opposite alterations of GLP-1R expression in glandular stomach. These results suggest that induced T1DM and T2DM may differently modulate GLP-1R system in enteropancreatic axis.http://dx.doi.org/10.1155/2015/561353 |
| spellingShingle | Olga Bloch Efrat Broide Gilad Ben-Yehudah Dror Cantrell Haim Shirin Micha J. Rapoport Nutrient Induced Type 2 and Chemical Induced Type 1 Experimental Diabetes Differently Modulate Gastric GLP-1 Receptor Expression Journal of Diabetes Research |
| title | Nutrient Induced Type 2 and Chemical Induced Type 1 Experimental Diabetes Differently Modulate Gastric GLP-1 Receptor Expression |
| title_full | Nutrient Induced Type 2 and Chemical Induced Type 1 Experimental Diabetes Differently Modulate Gastric GLP-1 Receptor Expression |
| title_fullStr | Nutrient Induced Type 2 and Chemical Induced Type 1 Experimental Diabetes Differently Modulate Gastric GLP-1 Receptor Expression |
| title_full_unstemmed | Nutrient Induced Type 2 and Chemical Induced Type 1 Experimental Diabetes Differently Modulate Gastric GLP-1 Receptor Expression |
| title_short | Nutrient Induced Type 2 and Chemical Induced Type 1 Experimental Diabetes Differently Modulate Gastric GLP-1 Receptor Expression |
| title_sort | nutrient induced type 2 and chemical induced type 1 experimental diabetes differently modulate gastric glp 1 receptor expression |
| url | http://dx.doi.org/10.1155/2015/561353 |
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