Synthesis and Degradation of the Phytohormone Indole-3-Acetic Acid by the Versatile Bacterium <i>Paraburkholderia xenovorans</i> LB400 and Its Growth Promotion of <i>Nicotiana tabacum</i> Plant
Plant growth-promoting bacteria (PGPB) play a role in stimulating plant growth through mechanisms such as the synthesis of the phytohormone indole-3-acetic acid (IAA). The aims of this study were the characterization of IAA synthesis and degradation by the model aromatic-degrading bacterium <i>...
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2024-12-01
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| author | Paulina Vega-Celedón Diyanira Castillo-Novales Guillermo Bravo Franco Cárdenas María José Romero-Silva Michael Seeger |
| author_facet | Paulina Vega-Celedón Diyanira Castillo-Novales Guillermo Bravo Franco Cárdenas María José Romero-Silva Michael Seeger |
| author_sort | Paulina Vega-Celedón |
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| description | Plant growth-promoting bacteria (PGPB) play a role in stimulating plant growth through mechanisms such as the synthesis of the phytohormone indole-3-acetic acid (IAA). The aims of this study were the characterization of IAA synthesis and degradation by the model aromatic-degrading bacterium <i>Paraburkholderia xenovorans</i> LB400, and its growth promotion of the <i>Nicotiana tabacum</i> plant. Strain LB400 was able to synthesize IAA (measured by HPLC) during growth in the presence of tryptophan and at least one additional carbon source; synthesis of anthranilic acid was also observed. RT-PCR analysis indicates that under these conditions, strain LB400 expressed the <i>ipdC</i> gene, which encodes indole-3-pyruvate decarboxylase, suggesting that IAA biosynthesis proceeds through the indole-3-pyruvate pathway. In addition, strain LB400 degraded IAA and grew on IAA as a sole carbon and energy source. Strain LB400 expressed the <i>iacC</i> and <i>catA</i> genes, which encode the α subunit of the aromatic-ring-hydroxylating dioxygenase in the IAA catabolic pathway and the catechol 1,2-dioxygenase, respectively, which may suggest a peripheral IAA pathway leading to the central catechol pathway. Notably, <i>P. xenovorans</i> LB400 promoted the growth of tobacco seedlings, increasing the number and the length of the roots. In conclusion, this study indicates that the versatile bacterium <i>P. xenovorans</i> LB400 is a PGPB. |
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| language | English |
| publishDate | 2024-12-01 |
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| series | Plants |
| spelling | doaj-art-7d2c00f707404a8b8a67264ef576c5ab2025-08-20T02:01:28ZengMDPI AGPlants2223-77472024-12-011324353310.3390/plants13243533Synthesis and Degradation of the Phytohormone Indole-3-Acetic Acid by the Versatile Bacterium <i>Paraburkholderia xenovorans</i> LB400 and Its Growth Promotion of <i>Nicotiana tabacum</i> PlantPaulina Vega-Celedón0Diyanira Castillo-Novales1Guillermo Bravo2Franco Cárdenas3María José Romero-Silva4Michael Seeger5Molecular Microbiology and Environmental Biotechnology Laboratory, Department of Chemistry, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso 2390123, ChileMolecular Microbiology and Environmental Biotechnology Laboratory, Department of Chemistry, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso 2390123, ChileMolecular Microbiology and Environmental Biotechnology Laboratory, Department of Chemistry, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso 2390123, ChileMolecular Microbiology and Environmental Biotechnology Laboratory, Department of Chemistry, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso 2390123, ChileMolecular Microbiology and Environmental Biotechnology Laboratory, Department of Chemistry, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso 2390123, ChileMolecular Microbiology and Environmental Biotechnology Laboratory, Department of Chemistry, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso 2390123, ChilePlant growth-promoting bacteria (PGPB) play a role in stimulating plant growth through mechanisms such as the synthesis of the phytohormone indole-3-acetic acid (IAA). The aims of this study were the characterization of IAA synthesis and degradation by the model aromatic-degrading bacterium <i>Paraburkholderia xenovorans</i> LB400, and its growth promotion of the <i>Nicotiana tabacum</i> plant. Strain LB400 was able to synthesize IAA (measured by HPLC) during growth in the presence of tryptophan and at least one additional carbon source; synthesis of anthranilic acid was also observed. RT-PCR analysis indicates that under these conditions, strain LB400 expressed the <i>ipdC</i> gene, which encodes indole-3-pyruvate decarboxylase, suggesting that IAA biosynthesis proceeds through the indole-3-pyruvate pathway. In addition, strain LB400 degraded IAA and grew on IAA as a sole carbon and energy source. Strain LB400 expressed the <i>iacC</i> and <i>catA</i> genes, which encode the α subunit of the aromatic-ring-hydroxylating dioxygenase in the IAA catabolic pathway and the catechol 1,2-dioxygenase, respectively, which may suggest a peripheral IAA pathway leading to the central catechol pathway. Notably, <i>P. xenovorans</i> LB400 promoted the growth of tobacco seedlings, increasing the number and the length of the roots. In conclusion, this study indicates that the versatile bacterium <i>P. xenovorans</i> LB400 is a PGPB.https://www.mdpi.com/2223-7747/13/24/3533indole-3-acetic acid (IAA)IAA synthesisIAA degradation<i>Paraburkholderia xenovorans</i> LB400plant growth-promoting bacteria (PGPB) |
| spellingShingle | Paulina Vega-Celedón Diyanira Castillo-Novales Guillermo Bravo Franco Cárdenas María José Romero-Silva Michael Seeger Synthesis and Degradation of the Phytohormone Indole-3-Acetic Acid by the Versatile Bacterium <i>Paraburkholderia xenovorans</i> LB400 and Its Growth Promotion of <i>Nicotiana tabacum</i> Plant Plants indole-3-acetic acid (IAA) IAA synthesis IAA degradation <i>Paraburkholderia xenovorans</i> LB400 plant growth-promoting bacteria (PGPB) |
| title | Synthesis and Degradation of the Phytohormone Indole-3-Acetic Acid by the Versatile Bacterium <i>Paraburkholderia xenovorans</i> LB400 and Its Growth Promotion of <i>Nicotiana tabacum</i> Plant |
| title_full | Synthesis and Degradation of the Phytohormone Indole-3-Acetic Acid by the Versatile Bacterium <i>Paraburkholderia xenovorans</i> LB400 and Its Growth Promotion of <i>Nicotiana tabacum</i> Plant |
| title_fullStr | Synthesis and Degradation of the Phytohormone Indole-3-Acetic Acid by the Versatile Bacterium <i>Paraburkholderia xenovorans</i> LB400 and Its Growth Promotion of <i>Nicotiana tabacum</i> Plant |
| title_full_unstemmed | Synthesis and Degradation of the Phytohormone Indole-3-Acetic Acid by the Versatile Bacterium <i>Paraburkholderia xenovorans</i> LB400 and Its Growth Promotion of <i>Nicotiana tabacum</i> Plant |
| title_short | Synthesis and Degradation of the Phytohormone Indole-3-Acetic Acid by the Versatile Bacterium <i>Paraburkholderia xenovorans</i> LB400 and Its Growth Promotion of <i>Nicotiana tabacum</i> Plant |
| title_sort | synthesis and degradation of the phytohormone indole 3 acetic acid by the versatile bacterium i paraburkholderia xenovorans i lb400 and its growth promotion of i nicotiana tabacum i plant |
| topic | indole-3-acetic acid (IAA) IAA synthesis IAA degradation <i>Paraburkholderia xenovorans</i> LB400 plant growth-promoting bacteria (PGPB) |
| url | https://www.mdpi.com/2223-7747/13/24/3533 |
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