Chromosome-level genome assembly of the vegetable leafminer Liriomyza sativae (Diptera: Agromyzidae)

Abstract So far, the chromosome-level genome assembly of only one species has been known for the family Agromyzidae. The vegetable leafminer, Liriomyza sativae Blanchard, 1938, is a highly polyphagous invasive pest that poses a significant threat to a wide range of vegetable crops. However, genomic...

Full description

Saved in:
Bibliographic Details
Main Authors: Xulong Chen, Xiaodong Cai, Jiuzhou Liu, Shuangmei Ding, Zheng Fan, Zhuo Chen, Ding Yang
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-05747-6
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract So far, the chromosome-level genome assembly of only one species has been known for the family Agromyzidae. The vegetable leafminer, Liriomyza sativae Blanchard, 1938, is a highly polyphagous invasive pest that poses a significant threat to a wide range of vegetable crops. However, genomic resources for this species remain unavailable, impeding our understanding of its invasive traits and extensive adaptability. In this study, we present the high-quality chromosome-level genome assembly of L. sativae, using MGI short-read, PacBio HiFi long-read and Hi-C sequencing technology. The final assembly spans 110.98 Mb, of which 92.08% (102.21 Mb) was anchored to five psedo-chromosomes, yielding a contig N50 of 1.16 Mb and 94.9% BUSCO completeness. The genome contains 30.39 Mb of repetitive sequences (accounting for 27.38% of the total) and encodes 10,312 predicted protein-coding genes, of which 9,978 (96.76%) are functionally annotated. The high-quality genome assembly provides a critical foundation for elucidating the adaptive evolution and invasion mechanisms of Liriomyza species.
ISSN:2052-4463