Nanovaccine loaded with seno-antigen target senescent cells to improve metabolic disorders of adipose tissue and cardiac dysfunction
The buildup of senescent cells exacerbates metabolic disorders in adipose tissue and contributes to aging-related cardiac dysfunction. Targeted clearance of senescent cells can markedly ameliorate these aging-related diseases. Here, we developed a novel nanovaccine (GK-NaV) loaded with seno-antigen...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Human Vaccines & Immunotherapeutics |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21645515.2025.2479229 |
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| author | Kexin Zhang Qiliang Yin Yucen Ma Mengyu Cao Lingwei Li Xinliang Jin Jiyan Leng |
| author_facet | Kexin Zhang Qiliang Yin Yucen Ma Mengyu Cao Lingwei Li Xinliang Jin Jiyan Leng |
| author_sort | Kexin Zhang |
| collection | DOAJ |
| description | The buildup of senescent cells exacerbates metabolic disorders in adipose tissue and contributes to aging-related cardiac dysfunction. Targeted clearance of senescent cells can markedly ameliorate these aging-related diseases. Here, we developed a novel nanovaccine (GK-NaV) loaded with seno-antigen that is self-assembled from the fusion of cationic protein (K36) and seno-antigen peptide (Gpnmb). The GK-NaV could be highly engulfed by bone marrow-derived dendritic cells (BMDCs) and efficiently present antigens on the cellular surface, thereby promoting DCs maturation and activation of CD8+T cells in vitro. Following subcutaneous immunization, GK-NaV not only exhibited a noticeable antigen depot effect but also markedly activated specific cellular immune responses, enhancing the immunoreactivity and cytotoxic effects of CD8+T cells. Consequently, the targeted anti-aging immunity triggered by GK-NaV demonstrated the ability to selectively eliminate senescent adipocytes and cardiomyocytes in high-fat diet (HFD)-induced progeroid mice, leading to a significant improvement in age-related metabolic disorders in adipose tissue and cardiac dysfunction. Hence, our findings indicate that immunization with GK-NaV targeting seno-antigens may represent a promising strategy for novel senolytic therapies. |
| format | Article |
| id | doaj-art-7d2268ffaa6942f0af9931b2fcc0cbb4 |
| institution | DOAJ |
| issn | 2164-5515 2164-554X |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Human Vaccines & Immunotherapeutics |
| spelling | doaj-art-7d2268ffaa6942f0af9931b2fcc0cbb42025-08-20T03:05:21ZengTaylor & Francis GroupHuman Vaccines & Immunotherapeutics2164-55152164-554X2025-12-0121110.1080/21645515.2025.2479229Nanovaccine loaded with seno-antigen target senescent cells to improve metabolic disorders of adipose tissue and cardiac dysfunctionKexin Zhang0Qiliang Yin1Yucen Ma2Mengyu Cao3Lingwei Li4Xinliang Jin5Jiyan Leng6Department of Cadre Ward, The First Hospital of Jilin University, Changchun, ChinaDepartment of Cadre Ward, The First Hospital of Jilin University, Changchun, ChinaDepartment of Cadre Ward, The First Hospital of Jilin University, Changchun, ChinaDepartment of Cadre Ward, The First Hospital of Jilin University, Changchun, ChinaDepartment of Cadre Ward, The First Hospital of Jilin University, Changchun, ChinaDepartment of General Surgery, First Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun, ChinaDepartment of Cadre Ward, The First Hospital of Jilin University, Changchun, ChinaThe buildup of senescent cells exacerbates metabolic disorders in adipose tissue and contributes to aging-related cardiac dysfunction. Targeted clearance of senescent cells can markedly ameliorate these aging-related diseases. Here, we developed a novel nanovaccine (GK-NaV) loaded with seno-antigen that is self-assembled from the fusion of cationic protein (K36) and seno-antigen peptide (Gpnmb). The GK-NaV could be highly engulfed by bone marrow-derived dendritic cells (BMDCs) and efficiently present antigens on the cellular surface, thereby promoting DCs maturation and activation of CD8+T cells in vitro. Following subcutaneous immunization, GK-NaV not only exhibited a noticeable antigen depot effect but also markedly activated specific cellular immune responses, enhancing the immunoreactivity and cytotoxic effects of CD8+T cells. Consequently, the targeted anti-aging immunity triggered by GK-NaV demonstrated the ability to selectively eliminate senescent adipocytes and cardiomyocytes in high-fat diet (HFD)-induced progeroid mice, leading to a significant improvement in age-related metabolic disorders in adipose tissue and cardiac dysfunction. Hence, our findings indicate that immunization with GK-NaV targeting seno-antigens may represent a promising strategy for novel senolytic therapies.https://www.tandfonline.com/doi/10.1080/21645515.2025.2479229Nanovaccineseno-antigensenescent cellsmetabolic disorderscardiac dysfunction |
| spellingShingle | Kexin Zhang Qiliang Yin Yucen Ma Mengyu Cao Lingwei Li Xinliang Jin Jiyan Leng Nanovaccine loaded with seno-antigen target senescent cells to improve metabolic disorders of adipose tissue and cardiac dysfunction Human Vaccines & Immunotherapeutics Nanovaccine seno-antigen senescent cells metabolic disorders cardiac dysfunction |
| title | Nanovaccine loaded with seno-antigen target senescent cells to improve metabolic disorders of adipose tissue and cardiac dysfunction |
| title_full | Nanovaccine loaded with seno-antigen target senescent cells to improve metabolic disorders of adipose tissue and cardiac dysfunction |
| title_fullStr | Nanovaccine loaded with seno-antigen target senescent cells to improve metabolic disorders of adipose tissue and cardiac dysfunction |
| title_full_unstemmed | Nanovaccine loaded with seno-antigen target senescent cells to improve metabolic disorders of adipose tissue and cardiac dysfunction |
| title_short | Nanovaccine loaded with seno-antigen target senescent cells to improve metabolic disorders of adipose tissue and cardiac dysfunction |
| title_sort | nanovaccine loaded with seno antigen target senescent cells to improve metabolic disorders of adipose tissue and cardiac dysfunction |
| topic | Nanovaccine seno-antigen senescent cells metabolic disorders cardiac dysfunction |
| url | https://www.tandfonline.com/doi/10.1080/21645515.2025.2479229 |
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