Natural regulatory T cells increase significantly in pediatric patients with parasitic infections: Flow cytometry study
Background: The most accepted definition of regulatory T cells (Tregs) relies on the expression of several biomarkers, including CD4, CD25, and transcription factor, Foxp3. The Tregs maintain tolerance to self-antigens and prevent autoimmune diseases. Aim: The purpose of this study was to determine...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2023-07-01
|
| Series: | Indian Journal of Pathology and Microbiology |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/ijpm.ijpm_1262_21 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1825206162657640448 |
|---|---|
| author | Nadeem Kizilbash Nida Suhail A. Khuzaim Alzahrani W. Jamith Basha Mohamed Soliman |
| author_facet | Nadeem Kizilbash Nida Suhail A. Khuzaim Alzahrani W. Jamith Basha Mohamed Soliman |
| author_sort | Nadeem Kizilbash |
| collection | DOAJ |
| description | Background:
The most accepted definition of regulatory T cells (Tregs) relies on the expression of several biomarkers, including CD4, CD25, and transcription factor, Foxp3. The Tregs maintain tolerance to self-antigens and prevent autoimmune diseases.
Aim:
The purpose of this study was to determine the difference in natural Treg levels in Entamoeba histolytica, Schistosoma mansoni, Giardia lamblia, Enterobius vermicularis, and Hymenolepis nana infected patients.
Setting and Design:
Fifty-one pediatric subjects (29 males and 22 females) were recruited from a tertiary care hospital, and were divided into infected and non-infected (control) groups. The mean age of the subjects was 8.7 years.
Materials and Methods:
Blood samples were collected from infected and non-infected groups, and change in the level of Tregs in these subjects was investigated by flow cytometry.
Statistical Analysis Used:
The statistical analysis of data was performed by SPSS software. Quantitative data used in this study included mean and standard deviation. Data from the two groups were compared by the Student's t-test. The age of the patient and infection status were used for multivariate logistic regression analysis. Odds ratios (ORs) were estimated within a 95% confidence interval, and a P value of <0.05 was considered significant.
Results and Conclusions:
The levels of natural regulatory T cells, indicated by the biomarkers, CD4+, CD25+, and Foxp3+, increase significantly in patients infected by Entamoeba histolytica, Schistosoma mansoni, Giardia lamblia, Enterobius vermicularis, and Hymenolepis nana as compared to controls. They also increase in cases of mixed infection as compared to infection by a single parasite. |
| format | Article |
| id | doaj-art-7d217dbec36f43fba2c3a6d1b2ac5dc3 |
| institution | Kabale University |
| issn | 0377-4929 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Pathology and Microbiology |
| spelling | doaj-art-7d217dbec36f43fba2c3a6d1b2ac5dc32025-02-07T13:31:52ZengWolters Kluwer Medknow PublicationsIndian Journal of Pathology and Microbiology0377-49292023-07-0166355655910.4103/ijpm.ijpm_1262_21Natural regulatory T cells increase significantly in pediatric patients with parasitic infections: Flow cytometry studyNadeem KizilbashNida SuhailA. Khuzaim AlzahraniW. Jamith BashaMohamed SolimanBackground: The most accepted definition of regulatory T cells (Tregs) relies on the expression of several biomarkers, including CD4, CD25, and transcription factor, Foxp3. The Tregs maintain tolerance to self-antigens and prevent autoimmune diseases. Aim: The purpose of this study was to determine the difference in natural Treg levels in Entamoeba histolytica, Schistosoma mansoni, Giardia lamblia, Enterobius vermicularis, and Hymenolepis nana infected patients. Setting and Design: Fifty-one pediatric subjects (29 males and 22 females) were recruited from a tertiary care hospital, and were divided into infected and non-infected (control) groups. The mean age of the subjects was 8.7 years. Materials and Methods: Blood samples were collected from infected and non-infected groups, and change in the level of Tregs in these subjects was investigated by flow cytometry. Statistical Analysis Used: The statistical analysis of data was performed by SPSS software. Quantitative data used in this study included mean and standard deviation. Data from the two groups were compared by the Student's t-test. The age of the patient and infection status were used for multivariate logistic regression analysis. Odds ratios (ORs) were estimated within a 95% confidence interval, and a P value of <0.05 was considered significant. Results and Conclusions: The levels of natural regulatory T cells, indicated by the biomarkers, CD4+, CD25+, and Foxp3+, increase significantly in patients infected by Entamoeba histolytica, Schistosoma mansoni, Giardia lamblia, Enterobius vermicularis, and Hymenolepis nana as compared to controls. They also increase in cases of mixed infection as compared to infection by a single parasite.https://journals.lww.com/10.4103/ijpm.ijpm_1262_21flow cytometryparasitic infectionpediatric patientsregulatory t cells |
| spellingShingle | Nadeem Kizilbash Nida Suhail A. Khuzaim Alzahrani W. Jamith Basha Mohamed Soliman Natural regulatory T cells increase significantly in pediatric patients with parasitic infections: Flow cytometry study Indian Journal of Pathology and Microbiology flow cytometry parasitic infection pediatric patients regulatory t cells |
| title | Natural regulatory T cells increase significantly in pediatric patients with parasitic infections: Flow cytometry study |
| title_full | Natural regulatory T cells increase significantly in pediatric patients with parasitic infections: Flow cytometry study |
| title_fullStr | Natural regulatory T cells increase significantly in pediatric patients with parasitic infections: Flow cytometry study |
| title_full_unstemmed | Natural regulatory T cells increase significantly in pediatric patients with parasitic infections: Flow cytometry study |
| title_short | Natural regulatory T cells increase significantly in pediatric patients with parasitic infections: Flow cytometry study |
| title_sort | natural regulatory t cells increase significantly in pediatric patients with parasitic infections flow cytometry study |
| topic | flow cytometry parasitic infection pediatric patients regulatory t cells |
| url | https://journals.lww.com/10.4103/ijpm.ijpm_1262_21 |
| work_keys_str_mv | AT nadeemkizilbash naturalregulatorytcellsincreasesignificantlyinpediatricpatientswithparasiticinfectionsflowcytometrystudy AT nidasuhail naturalregulatorytcellsincreasesignificantlyinpediatricpatientswithparasiticinfectionsflowcytometrystudy AT akhuzaimalzahrani naturalregulatorytcellsincreasesignificantlyinpediatricpatientswithparasiticinfectionsflowcytometrystudy AT wjamithbasha naturalregulatorytcellsincreasesignificantlyinpediatricpatientswithparasiticinfectionsflowcytometrystudy AT mohamedsoliman naturalregulatorytcellsincreasesignificantlyinpediatricpatientswithparasiticinfectionsflowcytometrystudy |