Molecular Detection and Phylogenetic Analysis of Cucumber Mosaic Virus Infecting Bhut Jolokia (Capsicum chinense Jacq.) of Assam
Prevalence of Cucumber mosaic virus (CMV) in Bhut Jolokia (Capsicum chinense Jacq.) in Assam was confirmedby reverse transcription-polymerase chain reaction (RT-PCR). The symptomatic expression of CMV infection onto Bhut Jolokia was confirmed as chlorotic yellow mosaic appearance of the leaves, s...
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| Language: | English |
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Puspa Publishing House
2019-08-01
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| Series: | International Journal of Economic Plants |
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| Online Access: | https://ojs.pphouse.org/index.php/IJEP/article/view/4600 |
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| author | Munmi Borah R. Gowtham Kumar Sundaresha Siddappa |
| author_facet | Munmi Borah R. Gowtham Kumar Sundaresha Siddappa |
| author_sort | Munmi Borah |
| collection | DOAJ |
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Prevalence of Cucumber mosaic virus (CMV) in Bhut Jolokia (Capsicum chinense Jacq.) in Assam was confirmedby reverse transcription-polymerase chain reaction (RT-PCR). The symptomatic expression of CMV infection onto Bhut Jolokia was confirmed as chlorotic yellow mosaic appearance of the leaves, smaller leaves, reduction of plant growth, mosaic and mottling of infected leaves, leaves showing shoe-string or filliform symptom and leaf puckering based on RT-PCR. This is the first report on molecular characterization of CMV in Assam, India. The RT-PCR diagnosis revealed presence of CMV with a band length of 593bp which has been obtained from CMV infected samples. The amplified coat protein gene of CMV was cloned and sequenced. Phylogenetic analysis of coat Protein gene of CMV showed close relatedness with African isolates of sub group IB and other isolates of the world.
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| format | Article |
| id | doaj-art-7d11e9c781c3432ab8fcef71751d7a95 |
| institution | DOAJ |
| issn | 2349-4735 |
| language | English |
| publishDate | 2019-08-01 |
| publisher | Puspa Publishing House |
| record_format | Article |
| series | International Journal of Economic Plants |
| spelling | doaj-art-7d11e9c781c3432ab8fcef71751d7a952025-08-20T02:39:19ZengPuspa Publishing HouseInternational Journal of Economic Plants2349-47352019-08-016Aug, 3Molecular Detection and Phylogenetic Analysis of Cucumber Mosaic Virus Infecting Bhut Jolokia (Capsicum chinense Jacq.) of AssamMunmi Borah0R. Gowtham Kumar1Sundaresha Siddappa2Plant Virology Laboratory, Department of Plant Pathology, Assam Agricultural University, Jorhat, Assam (785 013), IndiaPlant Virology Laboratory, Department of Plant Pathology, Assam Agricultural University, Jorhat, Assam (785 013), IndiaDivision of Crop Improvement, Central Potato Research Institute, Shimla, H.P. (171 001), India Prevalence of Cucumber mosaic virus (CMV) in Bhut Jolokia (Capsicum chinense Jacq.) in Assam was confirmedby reverse transcription-polymerase chain reaction (RT-PCR). The symptomatic expression of CMV infection onto Bhut Jolokia was confirmed as chlorotic yellow mosaic appearance of the leaves, smaller leaves, reduction of plant growth, mosaic and mottling of infected leaves, leaves showing shoe-string or filliform symptom and leaf puckering based on RT-PCR. This is the first report on molecular characterization of CMV in Assam, India. The RT-PCR diagnosis revealed presence of CMV with a band length of 593bp which has been obtained from CMV infected samples. The amplified coat protein gene of CMV was cloned and sequenced. Phylogenetic analysis of coat Protein gene of CMV showed close relatedness with African isolates of sub group IB and other isolates of the world. https://ojs.pphouse.org/index.php/IJEP/article/view/4600Assam, Capsicum chinense , cucumber mosaic virus, phylogenetic tree, RT-PCR |
| spellingShingle | Munmi Borah R. Gowtham Kumar Sundaresha Siddappa Molecular Detection and Phylogenetic Analysis of Cucumber Mosaic Virus Infecting Bhut Jolokia (Capsicum chinense Jacq.) of Assam International Journal of Economic Plants Assam, Capsicum chinense , cucumber mosaic virus, phylogenetic tree, RT-PCR |
| title | Molecular Detection and Phylogenetic Analysis of Cucumber Mosaic Virus Infecting Bhut Jolokia (Capsicum chinense Jacq.) of Assam |
| title_full | Molecular Detection and Phylogenetic Analysis of Cucumber Mosaic Virus Infecting Bhut Jolokia (Capsicum chinense Jacq.) of Assam |
| title_fullStr | Molecular Detection and Phylogenetic Analysis of Cucumber Mosaic Virus Infecting Bhut Jolokia (Capsicum chinense Jacq.) of Assam |
| title_full_unstemmed | Molecular Detection and Phylogenetic Analysis of Cucumber Mosaic Virus Infecting Bhut Jolokia (Capsicum chinense Jacq.) of Assam |
| title_short | Molecular Detection and Phylogenetic Analysis of Cucumber Mosaic Virus Infecting Bhut Jolokia (Capsicum chinense Jacq.) of Assam |
| title_sort | molecular detection and phylogenetic analysis of cucumber mosaic virus infecting bhut jolokia capsicum chinense jacq of assam |
| topic | Assam, Capsicum chinense , cucumber mosaic virus, phylogenetic tree, RT-PCR |
| url | https://ojs.pphouse.org/index.php/IJEP/article/view/4600 |
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