Antiproliferative Effects of Methanolic Extracts of Cryptocarya concinna Hance Roots on Oral Cancer Ca9-22 and CAL 27 Cell Lines Involving Apoptosis, ROS Induction, and Mitochondrial Depolarization
Cryptocarya-derived natural products were reported to have several biological effects such as the antiproliferation of some cancers. The possible antioral cancer effect of Cryptocarya-derived substances was little addressed as yet. In this study, we firstly used the methanolic extracts of C. concinn...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/180462 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850219202276753408 |
|---|---|
| author | Hurng-Wern Huang Yi-An Chung Hsun-Shuo Chang Jen-Yang Tang Ih-Sheng Chen Hsueh-Wei Chang |
| author_facet | Hurng-Wern Huang Yi-An Chung Hsun-Shuo Chang Jen-Yang Tang Ih-Sheng Chen Hsueh-Wei Chang |
| author_sort | Hurng-Wern Huang |
| collection | DOAJ |
| description | Cryptocarya-derived natural products were reported to have several biological effects such as the antiproliferation of some cancers. The possible antioral cancer effect of Cryptocarya-derived substances was little addressed as yet. In this study, we firstly used the methanolic extracts of C. concinna Hance roots (MECCrt) to evaluate its potential function in antioral cancer bioactivity. We found that MECCrt significantly reduced cell viability of two oral cancer Ca9-22 and CAL 27 cell lines in dose-responsive manners (P<0.01). The percentages of sub-G1 phase and annexin V-positive of MECCrt-treated Ca9-22 and CAL 27 cell lines significantly accumulated (P<0.01) in a dose-responsive manner as evidenced by flow cytometry. These apoptotic effects were associated with the findings that intracellular ROS generation was induced in MECCrt-treated Ca9-22 and CAL 27 cell lines in dose-responsive and time-dependent manners (P<0.01). In a dose-responsive manner, MECCrt also significantly reduced the mitochondrial membrane potential in these two cell lines (P<0.01–0.05). In conclusion, we demonstrated that MECCrt may have antiproliferative potential against oral cancer cells involving apoptosis, ROS generation, and mitochondria membrane depolarization. |
| format | Article |
| id | doaj-art-7d05d4d89f214102a015b4dbe03e9563 |
| institution | OA Journals |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-7d05d4d89f214102a015b4dbe03e95632025-08-20T02:07:27ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/180462180462Antiproliferative Effects of Methanolic Extracts of Cryptocarya concinna Hance Roots on Oral Cancer Ca9-22 and CAL 27 Cell Lines Involving Apoptosis, ROS Induction, and Mitochondrial DepolarizationHurng-Wern Huang0Yi-An Chung1Hsun-Shuo Chang2Jen-Yang Tang3Ih-Sheng Chen4Hsueh-Wei Chang5Institute of Biomedical Science, National Sun Yat-Sen University, Kaohsiung 80424, TaiwanInstitute of Biomedical Science, National Sun Yat-Sen University, Kaohsiung 80424, TaiwanGraduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, TaiwanDepartment of Radiation Oncology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, TaiwanGraduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, TaiwanCancer Center, Translational Research Center, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80708, TaiwanCryptocarya-derived natural products were reported to have several biological effects such as the antiproliferation of some cancers. The possible antioral cancer effect of Cryptocarya-derived substances was little addressed as yet. In this study, we firstly used the methanolic extracts of C. concinna Hance roots (MECCrt) to evaluate its potential function in antioral cancer bioactivity. We found that MECCrt significantly reduced cell viability of two oral cancer Ca9-22 and CAL 27 cell lines in dose-responsive manners (P<0.01). The percentages of sub-G1 phase and annexin V-positive of MECCrt-treated Ca9-22 and CAL 27 cell lines significantly accumulated (P<0.01) in a dose-responsive manner as evidenced by flow cytometry. These apoptotic effects were associated with the findings that intracellular ROS generation was induced in MECCrt-treated Ca9-22 and CAL 27 cell lines in dose-responsive and time-dependent manners (P<0.01). In a dose-responsive manner, MECCrt also significantly reduced the mitochondrial membrane potential in these two cell lines (P<0.01–0.05). In conclusion, we demonstrated that MECCrt may have antiproliferative potential against oral cancer cells involving apoptosis, ROS generation, and mitochondria membrane depolarization.http://dx.doi.org/10.1155/2014/180462 |
| spellingShingle | Hurng-Wern Huang Yi-An Chung Hsun-Shuo Chang Jen-Yang Tang Ih-Sheng Chen Hsueh-Wei Chang Antiproliferative Effects of Methanolic Extracts of Cryptocarya concinna Hance Roots on Oral Cancer Ca9-22 and CAL 27 Cell Lines Involving Apoptosis, ROS Induction, and Mitochondrial Depolarization The Scientific World Journal |
| title | Antiproliferative Effects of Methanolic Extracts of Cryptocarya concinna Hance Roots on Oral Cancer Ca9-22 and CAL 27 Cell Lines Involving Apoptosis, ROS Induction, and Mitochondrial Depolarization |
| title_full | Antiproliferative Effects of Methanolic Extracts of Cryptocarya concinna Hance Roots on Oral Cancer Ca9-22 and CAL 27 Cell Lines Involving Apoptosis, ROS Induction, and Mitochondrial Depolarization |
| title_fullStr | Antiproliferative Effects of Methanolic Extracts of Cryptocarya concinna Hance Roots on Oral Cancer Ca9-22 and CAL 27 Cell Lines Involving Apoptosis, ROS Induction, and Mitochondrial Depolarization |
| title_full_unstemmed | Antiproliferative Effects of Methanolic Extracts of Cryptocarya concinna Hance Roots on Oral Cancer Ca9-22 and CAL 27 Cell Lines Involving Apoptosis, ROS Induction, and Mitochondrial Depolarization |
| title_short | Antiproliferative Effects of Methanolic Extracts of Cryptocarya concinna Hance Roots on Oral Cancer Ca9-22 and CAL 27 Cell Lines Involving Apoptosis, ROS Induction, and Mitochondrial Depolarization |
| title_sort | antiproliferative effects of methanolic extracts of cryptocarya concinna hance roots on oral cancer ca9 22 and cal 27 cell lines involving apoptosis ros induction and mitochondrial depolarization |
| url | http://dx.doi.org/10.1155/2014/180462 |
| work_keys_str_mv | AT hurngwernhuang antiproliferativeeffectsofmethanolicextractsofcryptocaryaconcinnahancerootsonoralcancerca922andcal27celllinesinvolvingapoptosisrosinductionandmitochondrialdepolarization AT yianchung antiproliferativeeffectsofmethanolicextractsofcryptocaryaconcinnahancerootsonoralcancerca922andcal27celllinesinvolvingapoptosisrosinductionandmitochondrialdepolarization AT hsunshuochang antiproliferativeeffectsofmethanolicextractsofcryptocaryaconcinnahancerootsonoralcancerca922andcal27celllinesinvolvingapoptosisrosinductionandmitochondrialdepolarization AT jenyangtang antiproliferativeeffectsofmethanolicextractsofcryptocaryaconcinnahancerootsonoralcancerca922andcal27celllinesinvolvingapoptosisrosinductionandmitochondrialdepolarization AT ihshengchen antiproliferativeeffectsofmethanolicextractsofcryptocaryaconcinnahancerootsonoralcancerca922andcal27celllinesinvolvingapoptosisrosinductionandmitochondrialdepolarization AT hsuehweichang antiproliferativeeffectsofmethanolicextractsofcryptocaryaconcinnahancerootsonoralcancerca922andcal27celllinesinvolvingapoptosisrosinductionandmitochondrialdepolarization |