Comparative analysis of the growth differences between hybrid Ningdu Yellow chickens and their parentals
ABSTRACT: Although the local high quality chicken breed in China has excellent flavor, its growth rate is inferior to that of foreign breeds. To improve the growth rate of local chicken breeds, it is crucial to study the mechanism of chicken muscle development. Herein, Ningdu Yellow chicken was used...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124008186 |
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| author | Yanping Wu Cong Huang Yue Wei Zhaofeng Kang Weihong Zhang Jinfang Xie Ligen Xiong Min Zhou Guosheng Zhang Rongjun Chen |
| author_facet | Yanping Wu Cong Huang Yue Wei Zhaofeng Kang Weihong Zhang Jinfang Xie Ligen Xiong Min Zhou Guosheng Zhang Rongjun Chen |
| author_sort | Yanping Wu |
| collection | DOAJ |
| description | ABSTRACT: Although the local high quality chicken breed in China has excellent flavor, its growth rate is inferior to that of foreign breeds. To improve the growth rate of local chicken breeds, it is crucial to study the mechanism of chicken muscle development. Herein, Ningdu Yellow chicken was used as the research object, and a new hybrid breed (W) was obtained by crossing the G, H and D lines, which combined the excellent physiological characteristics of its parents. Combined analysis of Ningdu Yellow chickens and their parents was carried out. Chickens from 105-day-old lines (W, G, H) were selected, and breast meat and serum were extracted for transcriptome sequencing and metabolome determination to study their growth differences. The live weight, carcass weight, half-eviscerated weight, eviscerated weight, and breast muscle weight of W were significantly higher than those of G and H. Differential expression analysis identified 1700 differentially expressed genes (DEG), and gene ontology and kyoto encyclopedia of genes and genomes (KEGG) analyses identified 33 and 1 pathways related to growth and development and steroid biosynthesis, respectively. Next, pairwise analysis identified 57 KEGG pathways, among which the MAPK signaling, steroid hormone biosynthesis, tight junction, and PPAR signaling pathways were involved in growth and development. Cluster analysis found that genes highly expressed in the W group were associated with regulation of the actin cytoskeleton, riboflavin metabolism, steroid biosynthesis, and glycerophospholipid metabolism. The top 2 clusters obtained by protein-protein interaction analysis were important for the growth and development of chickens. Finally, the metabolomic analysis found key differentially accumulated metabolites (DAM) that might be account for the growth differences. Further integrated analysis identified key DEGs and DAMs that might be responsible for the observed growth differences. This study identified genes governing growth traits in Ningdu Yellow chickens, laying a theoretical foundation for the development of chicken breeding, the utilization of hybrid supporting lines, and promotion of the Chinese chicken industry. |
| format | Article |
| id | doaj-art-7cfb6e330f4a40fdbf6efedcf923b8fd |
| institution | OA Journals |
| issn | 0032-5791 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-7cfb6e330f4a40fdbf6efedcf923b8fd2025-08-20T02:37:45ZengElsevierPoultry Science0032-57912024-12-011031210423910.1016/j.psj.2024.104239Comparative analysis of the growth differences between hybrid Ningdu Yellow chickens and their parentalsYanping Wu0Cong Huang1Yue Wei2Zhaofeng Kang3Weihong Zhang4Jinfang Xie5Ligen Xiong6Min Zhou7Guosheng Zhang8Rongjun Chen9Department of Biological Technology, Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi, 330200, China; Corresponding author:Department of Biological Technology, Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi, 330200, ChinaDepartment of Biological Technology, Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi, 330200, ChinaDepartment of Biological Technology, Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi, 330200, ChinaDepartment of Biological Technology, Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi, 330200, ChinaDepartment of Biological Technology, Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi, 330200, ChinaDepartment of Biological Technology, Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi, 330200, ChinaDepartment of Biological Technology, Nanchang Normal University, Nanchang, Jiangxi, 330100, ChinaAgricultural Technology Extension Center of Jiangxi Province, Jiangxi Agricultural University, Nanchang, Jiangxi, 330046, ChinaDepartment of Biological Technology, Huida Industry Co., Ltd., Ningdu, Jiangxi, 342800, ChinaABSTRACT: Although the local high quality chicken breed in China has excellent flavor, its growth rate is inferior to that of foreign breeds. To improve the growth rate of local chicken breeds, it is crucial to study the mechanism of chicken muscle development. Herein, Ningdu Yellow chicken was used as the research object, and a new hybrid breed (W) was obtained by crossing the G, H and D lines, which combined the excellent physiological characteristics of its parents. Combined analysis of Ningdu Yellow chickens and their parents was carried out. Chickens from 105-day-old lines (W, G, H) were selected, and breast meat and serum were extracted for transcriptome sequencing and metabolome determination to study their growth differences. The live weight, carcass weight, half-eviscerated weight, eviscerated weight, and breast muscle weight of W were significantly higher than those of G and H. Differential expression analysis identified 1700 differentially expressed genes (DEG), and gene ontology and kyoto encyclopedia of genes and genomes (KEGG) analyses identified 33 and 1 pathways related to growth and development and steroid biosynthesis, respectively. Next, pairwise analysis identified 57 KEGG pathways, among which the MAPK signaling, steroid hormone biosynthesis, tight junction, and PPAR signaling pathways were involved in growth and development. Cluster analysis found that genes highly expressed in the W group were associated with regulation of the actin cytoskeleton, riboflavin metabolism, steroid biosynthesis, and glycerophospholipid metabolism. The top 2 clusters obtained by protein-protein interaction analysis were important for the growth and development of chickens. Finally, the metabolomic analysis found key differentially accumulated metabolites (DAM) that might be account for the growth differences. Further integrated analysis identified key DEGs and DAMs that might be responsible for the observed growth differences. This study identified genes governing growth traits in Ningdu Yellow chickens, laying a theoretical foundation for the development of chicken breeding, the utilization of hybrid supporting lines, and promotion of the Chinese chicken industry.http://www.sciencedirect.com/science/article/pii/S0032579124008186Ningdu yellow chickenhybridgrowthtranscriptomemetabolome |
| spellingShingle | Yanping Wu Cong Huang Yue Wei Zhaofeng Kang Weihong Zhang Jinfang Xie Ligen Xiong Min Zhou Guosheng Zhang Rongjun Chen Comparative analysis of the growth differences between hybrid Ningdu Yellow chickens and their parentals Poultry Science Ningdu yellow chicken hybrid growth transcriptome metabolome |
| title | Comparative analysis of the growth differences between hybrid Ningdu Yellow chickens and their parentals |
| title_full | Comparative analysis of the growth differences between hybrid Ningdu Yellow chickens and their parentals |
| title_fullStr | Comparative analysis of the growth differences between hybrid Ningdu Yellow chickens and their parentals |
| title_full_unstemmed | Comparative analysis of the growth differences between hybrid Ningdu Yellow chickens and their parentals |
| title_short | Comparative analysis of the growth differences between hybrid Ningdu Yellow chickens and their parentals |
| title_sort | comparative analysis of the growth differences between hybrid ningdu yellow chickens and their parentals |
| topic | Ningdu yellow chicken hybrid growth transcriptome metabolome |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124008186 |
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