Haplotype-resolved and gap-free genome of a floating aquatic plant from the Oryzeae tribe, Hygroryza aristata

Abstract Objectives Hygroryza aristata, an aquatic plant native to Southeast Asia, shows a high degree of adaptability to aquatic environments. H. aristata, which belongs to the Oryzeae tribe and is closely related to rice (Oryza sativa), holds potential for crop improvement, particularly in flood t...

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Main Authors: Li-Yao Yang, Li-Kun Huang, Jin-Bin Lin, Cun-Jing Xu, Wei-Qi Tang, Bi-Guang Huang
Format: Article
Language:English
Published: BMC 2025-03-01
Series:BMC Genomic Data
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Online Access:https://doi.org/10.1186/s12863-025-01314-5
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author Li-Yao Yang
Li-Kun Huang
Jin-Bin Lin
Cun-Jing Xu
Wei-Qi Tang
Bi-Guang Huang
author_facet Li-Yao Yang
Li-Kun Huang
Jin-Bin Lin
Cun-Jing Xu
Wei-Qi Tang
Bi-Guang Huang
author_sort Li-Yao Yang
collection DOAJ
description Abstract Objectives Hygroryza aristata, an aquatic plant native to Southeast Asia, shows a high degree of adaptability to aquatic environments. H. aristata, which belongs to the Oryzeae tribe and is closely related to rice (Oryza sativa), holds potential for crop improvement, particularly in flood tolerance. This study aimed to sequence and assemble the genome of H. aristata. Data description We assembled the genome of H. aristata using 31.91 Gb of Pacific Biosciences (PacBio) High-fidelity (HiFi) data and 22.36 Gb of ultra long Oxford Nanopore Technology (ONT) data, resulting in two gap-free haplotype genomes, hap1 (349.74 Mb) and hap2 (347.98 Mb), each with 12 chromosomes and 23 telomeres. The continuity of chromosomes was supported by High-throughput chromosome conformation capture (Hi-C) data. The assemblies demonstrated high completeness, with > 99.8% of coverage rates, 98.4% of Benchmarking Universal Single-Copy Orthologs (BUSCO) scores, and > 11.0 of Long Terminal Repeat Assembly Index (LAI) scores per haplotype. RNA sequencing (RNA-seq) data (176.06 Gb) of six tissues was generated for genome annotation, identifying 39,139 and 38,746 protein-coding genes in hap1 and hap2, respectively.
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spelling doaj-art-7cec6a150f3944f299290eeb7fd246db2025-08-20T02:10:20ZengBMCBMC Genomic Data2730-68442025-03-012611410.1186/s12863-025-01314-5Haplotype-resolved and gap-free genome of a floating aquatic plant from the Oryzeae tribe, Hygroryza aristataLi-Yao Yang0Li-Kun Huang1Jin-Bin Lin2Cun-Jing Xu3Wei-Qi Tang4Bi-Guang Huang5College of Geography and Oceanography, Minjiang UniversityXiamen Jointgene Biotechnology Co., LtdCollege of Geography and Oceanography, Minjiang UniversityCollege of Geography and Oceanography, Minjiang UniversityCollege of Geography and Oceanography, Minjiang UniversityKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry UniversityAbstract Objectives Hygroryza aristata, an aquatic plant native to Southeast Asia, shows a high degree of adaptability to aquatic environments. H. aristata, which belongs to the Oryzeae tribe and is closely related to rice (Oryza sativa), holds potential for crop improvement, particularly in flood tolerance. This study aimed to sequence and assemble the genome of H. aristata. Data description We assembled the genome of H. aristata using 31.91 Gb of Pacific Biosciences (PacBio) High-fidelity (HiFi) data and 22.36 Gb of ultra long Oxford Nanopore Technology (ONT) data, resulting in two gap-free haplotype genomes, hap1 (349.74 Mb) and hap2 (347.98 Mb), each with 12 chromosomes and 23 telomeres. The continuity of chromosomes was supported by High-throughput chromosome conformation capture (Hi-C) data. The assemblies demonstrated high completeness, with > 99.8% of coverage rates, 98.4% of Benchmarking Universal Single-Copy Orthologs (BUSCO) scores, and > 11.0 of Long Terminal Repeat Assembly Index (LAI) scores per haplotype. RNA sequencing (RNA-seq) data (176.06 Gb) of six tissues was generated for genome annotation, identifying 39,139 and 38,746 protein-coding genes in hap1 and hap2, respectively.https://doi.org/10.1186/s12863-025-01314-5Hygroryza aristataHaplotype-resolvedGap-freeGenome assemblyGenome annotationPacBio HiFi
spellingShingle Li-Yao Yang
Li-Kun Huang
Jin-Bin Lin
Cun-Jing Xu
Wei-Qi Tang
Bi-Guang Huang
Haplotype-resolved and gap-free genome of a floating aquatic plant from the Oryzeae tribe, Hygroryza aristata
BMC Genomic Data
Hygroryza aristata
Haplotype-resolved
Gap-free
Genome assembly
Genome annotation
PacBio HiFi
title Haplotype-resolved and gap-free genome of a floating aquatic plant from the Oryzeae tribe, Hygroryza aristata
title_full Haplotype-resolved and gap-free genome of a floating aquatic plant from the Oryzeae tribe, Hygroryza aristata
title_fullStr Haplotype-resolved and gap-free genome of a floating aquatic plant from the Oryzeae tribe, Hygroryza aristata
title_full_unstemmed Haplotype-resolved and gap-free genome of a floating aquatic plant from the Oryzeae tribe, Hygroryza aristata
title_short Haplotype-resolved and gap-free genome of a floating aquatic plant from the Oryzeae tribe, Hygroryza aristata
title_sort haplotype resolved and gap free genome of a floating aquatic plant from the oryzeae tribe hygroryza aristata
topic Hygroryza aristata
Haplotype-resolved
Gap-free
Genome assembly
Genome annotation
PacBio HiFi
url https://doi.org/10.1186/s12863-025-01314-5
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