Genetic diversity of Trichoderma spp. isolated from alkaline pistachio soils using morphological and molecular characteristics
The genetic diversity of Trichoderma species isolated from pistachio soils in Kerman Province was investigated. A total of 131 isolates were obtained and identified at the species level by analyzing their morphological characters. Eighty-seven isolates were identified as Trichoderma harzianum and fo...
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Shahid Bahonar University of Kerman
2024-12-01
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| Series: | Journal of Advances in Plant Protection |
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| Online Access: | https://japp.uk.ac.ir/article_4620_009b3456dda7a2f5d657efae04b6cb45.pdf |
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| author | Hossein Alaei Fahimeh Mirkhani Amirhossein Mohammadi Masoumeh Haghdel |
| author_facet | Hossein Alaei Fahimeh Mirkhani Amirhossein Mohammadi Masoumeh Haghdel |
| author_sort | Hossein Alaei |
| collection | DOAJ |
| description | The genetic diversity of Trichoderma species isolated from pistachio soils in Kerman Province was investigated. A total of 131 isolates were obtained and identified at the species level by analyzing their morphological characters. Eighty-seven isolates were identified as Trichoderma harzianum and forty-four isolates as Trichoderma aureoviride. The sequences of their ribosomal RNA (rRNA) gene internal transcribed spacers (ITS), were determined and used as phylogenetic markers. The complete ITS nucleotide sequences of 17 selected isolates of T. harzianum and T. aureroviride were determined. Minimal rDNA-ITS sequence variation was observed, and molecular informative differences between T. harzianum and T. aureroviride were not found in phylogenetic analysis.. RAPD-PCR using the four random primers, OPA4, OPA3, A-5, and Pr3, was evaluated as a technique to separate and differentiate these two species. The four primers produced DNA bands for the 16 Trichoderma isolates, of which 81 (95.3%) were polymorphic, and four (4.7%) were monomorphic. The RAPD data was computed into a similarity matrix using the NTSYS computer program. The genetic similarity coefficient between pairwise isolates varied from 0.50 to 0.98 based on an unweighted paired group method of arithmetic average (UPGMA) cluster analysis. The results showed that all isolates were grouped into two main clades. In agreement with observation, data from morphological characteristics resulting in RAPD-PCR banding patterns revealed it was easier to identify and differentiate T. harzianum and T. aureroviride isolates. The RAPD-PCR technique was practical and efficient for routine high-resolution diversity studies for the two species. |
| format | Article |
| id | doaj-art-7ce1b99751ab41cabffc85de7f3a2d5a |
| institution | DOAJ |
| issn | 3060-5954 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Shahid Bahonar University of Kerman |
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| series | Journal of Advances in Plant Protection |
| spelling | doaj-art-7ce1b99751ab41cabffc85de7f3a2d5a2025-08-20T03:01:45ZengShahid Bahonar University of KermanJournal of Advances in Plant Protection3060-59542024-12-0112839610.22103/japp.2024.24342.10204620Genetic diversity of Trichoderma spp. isolated from alkaline pistachio soils using morphological and molecular characteristicsHossein Alaei0Fahimeh Mirkhani1Amirhossein Mohammadi2Masoumeh Haghdel3Department of Plant Protection, Vali-e-Asr University of Rafsanjan, Rafsanjan, IranDepartment of Plant Protection, Vali-e-Asr University of Rafsanjan, Rafsanjan, IranPistachio Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rafsanjan, IranPistachio Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rafsanjan, IranThe genetic diversity of Trichoderma species isolated from pistachio soils in Kerman Province was investigated. A total of 131 isolates were obtained and identified at the species level by analyzing their morphological characters. Eighty-seven isolates were identified as Trichoderma harzianum and forty-four isolates as Trichoderma aureoviride. The sequences of their ribosomal RNA (rRNA) gene internal transcribed spacers (ITS), were determined and used as phylogenetic markers. The complete ITS nucleotide sequences of 17 selected isolates of T. harzianum and T. aureroviride were determined. Minimal rDNA-ITS sequence variation was observed, and molecular informative differences between T. harzianum and T. aureroviride were not found in phylogenetic analysis.. RAPD-PCR using the four random primers, OPA4, OPA3, A-5, and Pr3, was evaluated as a technique to separate and differentiate these two species. The four primers produced DNA bands for the 16 Trichoderma isolates, of which 81 (95.3%) were polymorphic, and four (4.7%) were monomorphic. The RAPD data was computed into a similarity matrix using the NTSYS computer program. The genetic similarity coefficient between pairwise isolates varied from 0.50 to 0.98 based on an unweighted paired group method of arithmetic average (UPGMA) cluster analysis. The results showed that all isolates were grouped into two main clades. In agreement with observation, data from morphological characteristics resulting in RAPD-PCR banding patterns revealed it was easier to identify and differentiate T. harzianum and T. aureroviride isolates. The RAPD-PCR technique was practical and efficient for routine high-resolution diversity studies for the two species.https://japp.uk.ac.ir/article_4620_009b3456dda7a2f5d657efae04b6cb45.pdfalkaline soilrandom primerrapd-pcrribosomal rna |
| spellingShingle | Hossein Alaei Fahimeh Mirkhani Amirhossein Mohammadi Masoumeh Haghdel Genetic diversity of Trichoderma spp. isolated from alkaline pistachio soils using morphological and molecular characteristics Journal of Advances in Plant Protection alkaline soil random primer rapd-pcr ribosomal rna |
| title | Genetic diversity of Trichoderma spp. isolated from alkaline pistachio soils using morphological and molecular characteristics |
| title_full | Genetic diversity of Trichoderma spp. isolated from alkaline pistachio soils using morphological and molecular characteristics |
| title_fullStr | Genetic diversity of Trichoderma spp. isolated from alkaline pistachio soils using morphological and molecular characteristics |
| title_full_unstemmed | Genetic diversity of Trichoderma spp. isolated from alkaline pistachio soils using morphological and molecular characteristics |
| title_short | Genetic diversity of Trichoderma spp. isolated from alkaline pistachio soils using morphological and molecular characteristics |
| title_sort | genetic diversity of trichoderma spp isolated from alkaline pistachio soils using morphological and molecular characteristics |
| topic | alkaline soil random primer rapd-pcr ribosomal rna |
| url | https://japp.uk.ac.ir/article_4620_009b3456dda7a2f5d657efae04b6cb45.pdf |
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