A draft genome assembly for the dart-poison frog Phyllobates terribilis
Dendrobatid poison frogs have become well established as model systems in several fields of biology. Nevertheless, the development of molecular and genetic resources for these frogs has been hindered by their large, highly repetitive genomes, which have proven difficult to assemble. Here we present...
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GigaScience Press
2025-06-01
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| Series: | GigaByte |
| Online Access: | https://gigabytejournal.com/articles/157 |
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| author | Roberto Márquez Denis Jacob Machado Reyhaneh Nouri Kerry L. Gendreau Daniel Janies Ralph A. Saporito Marcus R. Kronforst Taran Grant |
| author_facet | Roberto Márquez Denis Jacob Machado Reyhaneh Nouri Kerry L. Gendreau Daniel Janies Ralph A. Saporito Marcus R. Kronforst Taran Grant |
| author_sort | Roberto Márquez |
| collection | DOAJ |
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Dendrobatid poison frogs have become well established as model systems in several fields of biology. Nevertheless, the development of molecular and genetic resources for these frogs has been hindered by their large, highly repetitive genomes, which have proven difficult to assemble. Here we present a draft assembly for Phyllobates terribilis (12.6 Gb), generated using a combination of sequencing platforms and bioinformatic approaches. Similar to other poison frog sequencing efforts, we recovered a highly fragmented assembly, likely due to the genome’s large size and very high repeat content, which we estimated to be ≈88%. Despite the assembly’s low contiguity, we were able to annotate multiple members of three gene sets of interest (voltage-gated sodium channels and Notch and Wnt signaling pathways), demonstrating the usefulness of our assembly to the amphibian research community.
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| format | Article |
| id | doaj-art-7cddcd1eb30f4358904f81abd900b1bd |
| institution | OA Journals |
| issn | 2709-4715 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | GigaScience Press |
| record_format | Article |
| series | GigaByte |
| spelling | doaj-art-7cddcd1eb30f4358904f81abd900b1bd2025-08-20T02:21:21ZengGigaScience PressGigaByte2709-47152025-06-0110.46471/gigabyte.157A draft genome assembly for the dart-poison frog Phyllobates terribilisRoberto Márquez 0https://orcid.org/0000-0002-0644-3078Denis Jacob Machado 1https://orcid.org/0000-0001-9858-4515Reyhaneh Nouri 2Kerry L. Gendreau 3https://orcid.org/0000-0001-6058-0893Daniel Janies 4Ralph A. Saporito 5Marcus R. Kronforst 6https://orcid.org/0000-0002-9825-891XTaran Grant 7Department of Biological Sciences, https://ror.org/02smfhw86Virginia Tech, Blacksburg, VA, USACenter for Computational Intelligence to Predict Health and Environmental Risks, https://ror.org/04dawnj30University of North Carolina at Charlotte, Charlotte, NC, USA, Department of Bioinformatics and Genomics, https://ror.org/04dawnj30University of North Carolina at Charlotte, Charlotte, NC, USA, Laboratório de Anfíbios, Departamento de Zoologia, Instituto de Biociências, https://ror.org/036rp1748Universidade de São Paulo, São Paulo, BrazilCenter for Computational Intelligence to Predict Health and Environmental Risks, https://ror.org/04dawnj30University of North Carolina at Charlotte, Charlotte, NC, USA, Department of Bioinformatics and Genomics, https://ror.org/04dawnj30University of North Carolina at Charlotte, Charlotte, NC, USADepartment of Biological Sciences, https://ror.org/02smfhw86Virginia Tech, Blacksburg, VA, USACenter for Computational Intelligence to Predict Health and Environmental Risks, https://ror.org/04dawnj30University of North Carolina at Charlotte, Charlotte, NC, USA, Department of Bioinformatics and Genomics, https://ror.org/04dawnj30University of North Carolina at Charlotte, Charlotte, NC, USADepartment of Biology, https://ror.org/001gmya32John Carroll University, University Heights, OH, USADepartment of Ecology and Evolution, https://ror.org/024mw5h28University of Chicago, Chicago, IL, USALaboratório de Anfíbios, Departamento de Zoologia, Instituto de Biociências, https://ror.org/036rp1748Universidade de São Paulo, São Paulo, Brazil Dendrobatid poison frogs have become well established as model systems in several fields of biology. Nevertheless, the development of molecular and genetic resources for these frogs has been hindered by their large, highly repetitive genomes, which have proven difficult to assemble. Here we present a draft assembly for Phyllobates terribilis (12.6 Gb), generated using a combination of sequencing platforms and bioinformatic approaches. Similar to other poison frog sequencing efforts, we recovered a highly fragmented assembly, likely due to the genome’s large size and very high repeat content, which we estimated to be ≈88%. Despite the assembly’s low contiguity, we were able to annotate multiple members of three gene sets of interest (voltage-gated sodium channels and Notch and Wnt signaling pathways), demonstrating the usefulness of our assembly to the amphibian research community. https://gigabytejournal.com/articles/157 |
| spellingShingle | Roberto Márquez Denis Jacob Machado Reyhaneh Nouri Kerry L. Gendreau Daniel Janies Ralph A. Saporito Marcus R. Kronforst Taran Grant A draft genome assembly for the dart-poison frog Phyllobates terribilis GigaByte |
| title | A draft genome assembly for the dart-poison frog Phyllobates terribilis |
| title_full | A draft genome assembly for the dart-poison frog Phyllobates terribilis |
| title_fullStr | A draft genome assembly for the dart-poison frog Phyllobates terribilis |
| title_full_unstemmed | A draft genome assembly for the dart-poison frog Phyllobates terribilis |
| title_short | A draft genome assembly for the dart-poison frog Phyllobates terribilis |
| title_sort | draft genome assembly for the dart poison frog phyllobates terribilis |
| url | https://gigabytejournal.com/articles/157 |
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