Aptamer-Based Microfluidic Assay for In-Field Detection of Salicylic Acid in <i>Botrytis cinerea</i>-Infected Strawberries
Rapid detection of plant infections is crucial for minimising crop loss and optimising management strategies, particularly in the context of climate change. While traditional diagnostic methods provide precise measurements of phytohormones such as salicylic acid (SA), a key regulator of plant defenc...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-04-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/15/5/266 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849711794718769152 |
|---|---|
| author | Cristiana Domingues Rafaela R. Rosa Rodolfo G. Rodrigues Ana Margarida Fortes Virginia Chu João Pedro Conde |
| author_facet | Cristiana Domingues Rafaela R. Rosa Rodolfo G. Rodrigues Ana Margarida Fortes Virginia Chu João Pedro Conde |
| author_sort | Cristiana Domingues |
| collection | DOAJ |
| description | Rapid detection of plant infections is crucial for minimising crop loss and optimising management strategies, particularly in the context of climate change. While traditional diagnostic methods provide precise measurements of phytohormones such as salicylic acid (SA), a key regulator of plant defence responses, their reliance on bulky equipment and lengthy analysis times limits field applicability. This study presents a microfluidic-based aptamer assay for SA detection, enabling rapid and sensitive fluorescence-based readout from plant samples. A tailored sample pre-treatment protocol was developed and validated with real strawberry samples using HPLC measurements. The assay demonstrated a detection limit ranging from 10<sup>−9</sup> to 10<sup>−6</sup> mg/mL, within the relevant range for early infection diagnosis. The integration of the microfluidic platform with the optimised pre-treatment protocol offers a portable, cost-effective solution for on-site phytohormone analysis, providing a valuable tool for early infection detection and improved crop management. |
| format | Article |
| id | doaj-art-7cdd8afd9e4642c89a39b42582999b86 |
| institution | DOAJ |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-7cdd8afd9e4642c89a39b42582999b862025-08-20T03:14:32ZengMDPI AGBiosensors2079-63742025-04-0115526610.3390/bios15050266Aptamer-Based Microfluidic Assay for In-Field Detection of Salicylic Acid in <i>Botrytis cinerea</i>-Infected StrawberriesCristiana Domingues0Rafaela R. Rosa1Rodolfo G. Rodrigues2Ana Margarida Fortes3Virginia Chu4João Pedro Conde5Instituto de Engenharia de Sistemas e Computadores–Microsistemas e Nanotecnologias (INESC-MN), Rua Alves Redol, 1000-029 Lisbon, PortugalInstituto de Engenharia de Sistemas e Computadores–Microsistemas e Nanotecnologias (INESC-MN), Rua Alves Redol, 1000-029 Lisbon, PortugalInstituto de Engenharia de Sistemas e Computadores–Microsistemas e Nanotecnologias (INESC-MN), Rua Alves Redol, 1000-029 Lisbon, PortugalInstituto de Biossistemas e Ciências Integrativas (BioISI), Faculdade de Ciências de Lisboa, Universidade de Lisboa, 1749-016 Lisbon, PortugalInstituto de Engenharia de Sistemas e Computadores–Microsistemas e Nanotecnologias (INESC-MN), Rua Alves Redol, 1000-029 Lisbon, PortugalInstituto de Engenharia de Sistemas e Computadores–Microsistemas e Nanotecnologias (INESC-MN), Rua Alves Redol, 1000-029 Lisbon, PortugalRapid detection of plant infections is crucial for minimising crop loss and optimising management strategies, particularly in the context of climate change. While traditional diagnostic methods provide precise measurements of phytohormones such as salicylic acid (SA), a key regulator of plant defence responses, their reliance on bulky equipment and lengthy analysis times limits field applicability. This study presents a microfluidic-based aptamer assay for SA detection, enabling rapid and sensitive fluorescence-based readout from plant samples. A tailored sample pre-treatment protocol was developed and validated with real strawberry samples using HPLC measurements. The assay demonstrated a detection limit ranging from 10<sup>−9</sup> to 10<sup>−6</sup> mg/mL, within the relevant range for early infection diagnosis. The integration of the microfluidic platform with the optimised pre-treatment protocol offers a portable, cost-effective solution for on-site phytohormone analysis, providing a valuable tool for early infection detection and improved crop management.https://www.mdpi.com/2079-6374/15/5/266microfluidicssalicylic acidaptamer-based assayoptical sensoragriculture |
| spellingShingle | Cristiana Domingues Rafaela R. Rosa Rodolfo G. Rodrigues Ana Margarida Fortes Virginia Chu João Pedro Conde Aptamer-Based Microfluidic Assay for In-Field Detection of Salicylic Acid in <i>Botrytis cinerea</i>-Infected Strawberries Biosensors microfluidics salicylic acid aptamer-based assay optical sensor agriculture |
| title | Aptamer-Based Microfluidic Assay for In-Field Detection of Salicylic Acid in <i>Botrytis cinerea</i>-Infected Strawberries |
| title_full | Aptamer-Based Microfluidic Assay for In-Field Detection of Salicylic Acid in <i>Botrytis cinerea</i>-Infected Strawberries |
| title_fullStr | Aptamer-Based Microfluidic Assay for In-Field Detection of Salicylic Acid in <i>Botrytis cinerea</i>-Infected Strawberries |
| title_full_unstemmed | Aptamer-Based Microfluidic Assay for In-Field Detection of Salicylic Acid in <i>Botrytis cinerea</i>-Infected Strawberries |
| title_short | Aptamer-Based Microfluidic Assay for In-Field Detection of Salicylic Acid in <i>Botrytis cinerea</i>-Infected Strawberries |
| title_sort | aptamer based microfluidic assay for in field detection of salicylic acid in i botrytis cinerea i infected strawberries |
| topic | microfluidics salicylic acid aptamer-based assay optical sensor agriculture |
| url | https://www.mdpi.com/2079-6374/15/5/266 |
| work_keys_str_mv | AT cristianadomingues aptamerbasedmicrofluidicassayforinfielddetectionofsalicylicacidinibotrytiscinereaiinfectedstrawberries AT rafaelarrosa aptamerbasedmicrofluidicassayforinfielddetectionofsalicylicacidinibotrytiscinereaiinfectedstrawberries AT rodolfogrodrigues aptamerbasedmicrofluidicassayforinfielddetectionofsalicylicacidinibotrytiscinereaiinfectedstrawberries AT anamargaridafortes aptamerbasedmicrofluidicassayforinfielddetectionofsalicylicacidinibotrytiscinereaiinfectedstrawberries AT virginiachu aptamerbasedmicrofluidicassayforinfielddetectionofsalicylicacidinibotrytiscinereaiinfectedstrawberries AT joaopedroconde aptamerbasedmicrofluidicassayforinfielddetectionofsalicylicacidinibotrytiscinereaiinfectedstrawberries |