The effect of age and a high-fat, high-carbohydrate diet on the development of arterial hypertension and kidney disease in the experiment
Aim. To identify the structural foundations of the pathogenesis of arterial hypertension and kidney disease associated with a high-fat, high-carbohydrate diet and age.Materials and methods. The study was carried out on male Wistar rats aged 60 and 450 days. The animals were divided into 4 groups: gr...
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Siberian State Medical University (Tomsk)
2022-10-01
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| Series: | Бюллетень сибирской медицины |
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| Online Access: | https://bulletin.ssmu.ru/jour/article/view/4909 |
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| author | L. R. Mustafina S. V. Logvinov N. V. Naryzhnaya B. K. Kurbatov L. N. Maslov |
| author_facet | L. R. Mustafina S. V. Logvinov N. V. Naryzhnaya B. K. Kurbatov L. N. Maslov |
| author_sort | L. R. Mustafina |
| collection | DOAJ |
| description | Aim. To identify the structural foundations of the pathogenesis of arterial hypertension and kidney disease associated with a high-fat, high-carbohydrate diet and age.Materials and methods. The study was carried out on male Wistar rats aged 60 and 450 days. The animals were divided into 4 groups: group 1 (n = 14) – intact rats (60 days old) fed with a standard diet for 90 days; group 2 (n = 14) – rats (aged 60 days) receiving a high-fat, high-carbohydrate diet for 90 days; group 3 (n = 14) – intact rats (aged 450 days) receiving a standard diet for 90 days; group 4 (n = 14) – rats (aged 450 days) fed with a high-fat, high-carbohydrate diet for 90 days. Clinical and instrumental research methods, enzyme-linked immunosorbent assay, and immunohistochemistry and histology techniques were used in the study.Results. Feeding 60-day-old animals with a high-fat, high-carbohydrate diet resulted in an increase in body weight and abdominal fat, a rise in systolic blood pressure, and moderately pronounced histologic changes in the kidneys. In intact 450-day-old rats, age-related changes prevailed: changes in the myocardial mass, an increase in TGF-β1, morphological changes in the renal tubules and glomeruli. In 450-day-old rats receiving a high-fat, highcarbohydrate diet, the most pronounced increase in both systolic and diastolic blood pressure, a significant rise in serum fibronectin, and destructive changes in the renal tissue were noted.Conclusion. Functional and biochemical signs of arterial hypertension and morphological changes in the kidneys were the most pronounced in 450-day-old rats fed with a high-fat, high-carbohydrate diet. |
| format | Article |
| id | doaj-art-7cdb9fa40a2c4521aefb6b586a82a823 |
| institution | Kabale University |
| issn | 1682-0363 1819-3684 |
| language | English |
| publishDate | 2022-10-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj-art-7cdb9fa40a2c4521aefb6b586a82a8232025-08-20T03:37:46ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842022-10-01213738010.20538/1682-0363-2022-3-73-802919The effect of age and a high-fat, high-carbohydrate diet on the development of arterial hypertension and kidney disease in the experimentL. R. Mustafina0S. V. Logvinov1N. V. Naryzhnaya2B. K. Kurbatov3L. N. Maslov4Siberian State Medical UniversitySiberian State Medical UniversityCardiology Research Institute, Tomsk National Research Medical Center (NRMC), Russian Academy of SciencesCardiology Research Institute, Tomsk National Research Medical Center (NRMC), Russian Academy of SciencesCardiology Research Institute, Tomsk National Research Medical Center (NRMC), Russian Academy of SciencesAim. To identify the structural foundations of the pathogenesis of arterial hypertension and kidney disease associated with a high-fat, high-carbohydrate diet and age.Materials and methods. The study was carried out on male Wistar rats aged 60 and 450 days. The animals were divided into 4 groups: group 1 (n = 14) – intact rats (60 days old) fed with a standard diet for 90 days; group 2 (n = 14) – rats (aged 60 days) receiving a high-fat, high-carbohydrate diet for 90 days; group 3 (n = 14) – intact rats (aged 450 days) receiving a standard diet for 90 days; group 4 (n = 14) – rats (aged 450 days) fed with a high-fat, high-carbohydrate diet for 90 days. Clinical and instrumental research methods, enzyme-linked immunosorbent assay, and immunohistochemistry and histology techniques were used in the study.Results. Feeding 60-day-old animals with a high-fat, high-carbohydrate diet resulted in an increase in body weight and abdominal fat, a rise in systolic blood pressure, and moderately pronounced histologic changes in the kidneys. In intact 450-day-old rats, age-related changes prevailed: changes in the myocardial mass, an increase in TGF-β1, morphological changes in the renal tubules and glomeruli. In 450-day-old rats receiving a high-fat, highcarbohydrate diet, the most pronounced increase in both systolic and diastolic blood pressure, a significant rise in serum fibronectin, and destructive changes in the renal tissue were noted.Conclusion. Functional and biochemical signs of arterial hypertension and morphological changes in the kidneys were the most pronounced in 450-day-old rats fed with a high-fat, high-carbohydrate diet.https://bulletin.ssmu.ru/jour/article/view/4909arterial hypertensionage-related changes in the kidneyshigh-fathigh-carbohydrate diet |
| spellingShingle | L. R. Mustafina S. V. Logvinov N. V. Naryzhnaya B. K. Kurbatov L. N. Maslov The effect of age and a high-fat, high-carbohydrate diet on the development of arterial hypertension and kidney disease in the experiment Бюллетень сибирской медицины arterial hypertension age-related changes in the kidneys high-fat high-carbohydrate diet |
| title | The effect of age and a high-fat, high-carbohydrate diet on the development of arterial hypertension and kidney disease in the experiment |
| title_full | The effect of age and a high-fat, high-carbohydrate diet on the development of arterial hypertension and kidney disease in the experiment |
| title_fullStr | The effect of age and a high-fat, high-carbohydrate diet on the development of arterial hypertension and kidney disease in the experiment |
| title_full_unstemmed | The effect of age and a high-fat, high-carbohydrate diet on the development of arterial hypertension and kidney disease in the experiment |
| title_short | The effect of age and a high-fat, high-carbohydrate diet on the development of arterial hypertension and kidney disease in the experiment |
| title_sort | effect of age and a high fat high carbohydrate diet on the development of arterial hypertension and kidney disease in the experiment |
| topic | arterial hypertension age-related changes in the kidneys high-fat high-carbohydrate diet |
| url | https://bulletin.ssmu.ru/jour/article/view/4909 |
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