Degradation of High Concentrations of Anthracene Using White-Rot Wood-Inhabiting Fungi and Investigation of Enzyme Activities
Owing to the production of lignin-modifying enzymes (LMEs), white-rot fungi (WRF) such as polypores are potent organisms in the biodegradation of xenobiotic pollutants. The nonspecific function of LMEs including laccase and manganese peroxidase (MnP), has enabled the use of WRF in biotechnological a...
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Taylor & Francis Group
2024-09-01
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| Series: | Mycobiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/12298093.2024.2409485 |
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| author | Mohadeseh Khajehzadeh Masoomeh Ghobad-Nejhad Hamid Moghimi Ali Abolhasani Soorki Yu-Cheng Dai Jing Si |
| author_facet | Mohadeseh Khajehzadeh Masoomeh Ghobad-Nejhad Hamid Moghimi Ali Abolhasani Soorki Yu-Cheng Dai Jing Si |
| author_sort | Mohadeseh Khajehzadeh |
| collection | DOAJ |
| description | Owing to the production of lignin-modifying enzymes (LMEs), white-rot fungi (WRF) such as polypores are potent organisms in the biodegradation of xenobiotic pollutants. The nonspecific function of LMEs including laccase and manganese peroxidase (MnP), has enabled the use of WRF in biotechnological applications, particularly in bioremediation. In this study, 12 strains from nine white-rot basidiomycete genera viz., Ganoderma, Inocutis, Irpex, Lentinus, Lenzites, Oxyporus, Peniophora, Sanghuangporus, and Trametes were isolated from Iran and identified using morphological and molecular tools. The enzyme activity of laccase and manganese peroxidase that directly correlated with the biodegradation were determined, and the strains with the highest enzyme activities were evaluated for their ability to degrade 400 mg/L of anthracene over 28 days. Gas chromatography with flame ionization detector (GC-FID) revealed that four polypores viz., Trametes versicolor v21te, T. versicolor v22da, T. hirsuta, and Oxyporus sp. degraded 64%, 52%, 34%, and 20% of the anthracene, respectively. According to our analysis, the examined indigenous WRF are potentially useful candidates for the development of new mycoremediation techniques to degrade polycyclic aromatic hydrocarbons (PAHs). |
| format | Article |
| id | doaj-art-7cd7c9a862e54e6f821c501806ecbdd0 |
| institution | Kabale University |
| issn | 1229-8093 2092-9323 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Taylor & Francis Group |
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| series | Mycobiology |
| spelling | doaj-art-7cd7c9a862e54e6f821c501806ecbdd02025-01-19T13:50:12ZengTaylor & Francis GroupMycobiology1229-80932092-93232024-09-0152529830510.1080/12298093.2024.2409485Degradation of High Concentrations of Anthracene Using White-Rot Wood-Inhabiting Fungi and Investigation of Enzyme ActivitiesMohadeseh Khajehzadeh0Masoomeh Ghobad-Nejhad1Hamid Moghimi2Ali Abolhasani Soorki3Yu-Cheng Dai4Jing Si5Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, IranDepartment of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, IranDepartment of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, IranDepartment of Petroleum Microbiology, Research Institute of Applied Sciences, ACECR, Shahid Beheshti University, Tehran, IranState Key Laboratory of Efficient Production of Forest Resources, School of Ecology and Nature Conservation, Beijing Forestry University, Beijing, ChinaState Key Laboratory of Efficient Production of Forest Resources, School of Ecology and Nature Conservation, Beijing Forestry University, Beijing, ChinaOwing to the production of lignin-modifying enzymes (LMEs), white-rot fungi (WRF) such as polypores are potent organisms in the biodegradation of xenobiotic pollutants. The nonspecific function of LMEs including laccase and manganese peroxidase (MnP), has enabled the use of WRF in biotechnological applications, particularly in bioremediation. In this study, 12 strains from nine white-rot basidiomycete genera viz., Ganoderma, Inocutis, Irpex, Lentinus, Lenzites, Oxyporus, Peniophora, Sanghuangporus, and Trametes were isolated from Iran and identified using morphological and molecular tools. The enzyme activity of laccase and manganese peroxidase that directly correlated with the biodegradation were determined, and the strains with the highest enzyme activities were evaluated for their ability to degrade 400 mg/L of anthracene over 28 days. Gas chromatography with flame ionization detector (GC-FID) revealed that four polypores viz., Trametes versicolor v21te, T. versicolor v22da, T. hirsuta, and Oxyporus sp. degraded 64%, 52%, 34%, and 20% of the anthracene, respectively. According to our analysis, the examined indigenous WRF are potentially useful candidates for the development of new mycoremediation techniques to degrade polycyclic aromatic hydrocarbons (PAHs).https://www.tandfonline.com/doi/10.1080/12298093.2024.2409485Basidiomycetesenzyme activitylaccaseMycoremediationPolycyclic aromatic hydrocarbonspolypores |
| spellingShingle | Mohadeseh Khajehzadeh Masoomeh Ghobad-Nejhad Hamid Moghimi Ali Abolhasani Soorki Yu-Cheng Dai Jing Si Degradation of High Concentrations of Anthracene Using White-Rot Wood-Inhabiting Fungi and Investigation of Enzyme Activities Mycobiology Basidiomycetes enzyme activity laccase Mycoremediation Polycyclic aromatic hydrocarbons polypores |
| title | Degradation of High Concentrations of Anthracene Using White-Rot Wood-Inhabiting Fungi and Investigation of Enzyme Activities |
| title_full | Degradation of High Concentrations of Anthracene Using White-Rot Wood-Inhabiting Fungi and Investigation of Enzyme Activities |
| title_fullStr | Degradation of High Concentrations of Anthracene Using White-Rot Wood-Inhabiting Fungi and Investigation of Enzyme Activities |
| title_full_unstemmed | Degradation of High Concentrations of Anthracene Using White-Rot Wood-Inhabiting Fungi and Investigation of Enzyme Activities |
| title_short | Degradation of High Concentrations of Anthracene Using White-Rot Wood-Inhabiting Fungi and Investigation of Enzyme Activities |
| title_sort | degradation of high concentrations of anthracene using white rot wood inhabiting fungi and investigation of enzyme activities |
| topic | Basidiomycetes enzyme activity laccase Mycoremediation Polycyclic aromatic hydrocarbons polypores |
| url | https://www.tandfonline.com/doi/10.1080/12298093.2024.2409485 |
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