Construction and high-throughput screening of gradient nanowire coatings on titanium surface towards ameliorated osseointegration
Surface nano-modification has emerged as an effective strategy to enhance osseointegration of titanium (Ti) implants. Despite its promise, rational optimization of surface nanomorphology for ameliorated osseointegration remains a significant challenge. Our research pioneering developed a one-step al...
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Elsevier
2025-02-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006424004538 |
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| author | Ruiyue Hang Yuyu Zhao Huanming Chen Xiaomei Li Runhua Yao Yonghua Sun Xiaohong Yao Long Bai Huaiyu Wang Yong Han Ruiqiang Hang |
| author_facet | Ruiyue Hang Yuyu Zhao Huanming Chen Xiaomei Li Runhua Yao Yonghua Sun Xiaohong Yao Long Bai Huaiyu Wang Yong Han Ruiqiang Hang |
| author_sort | Ruiyue Hang |
| collection | DOAJ |
| description | Surface nano-modification has emerged as an effective strategy to enhance osseointegration of titanium (Ti) implants. Despite its promise, rational optimization of surface nanomorphology for ameliorated osseointegration remains a significant challenge. Our research pioneering developed a one-step alkali etching technique to produce a gradient nanowire coating with continuously varied dimensions on Ti surfaces, which was subsequently served as a versatile platform for high-throughput screening of optimal dimensions to enhance osseointegration. The results showed that macrophages (MФs) that mainly governed the initial inflammatory reaction exhibited a polarization tendency towards pro-healing M2 phenotype with decreased nanowire dimension due to nanomorphology-mediated focal adhesion formation and activation of its downstream signaling pathways (typically PI3K-Akt). Simultaneously, small-sized nanowires with diameter of 5.63–14.25 nm and inter-spacing of 29.42–57.97 nm were conductive to angiogenesis of endothelial cells (ECs) and osteogenesis of bone marrow mesenchymal stem cells (BMSCs), which may share similar mechanisms of MФs. The in vivo results well corroborated these in vitro observations. The knowledge gained from the present work not only advance our understanding of the interaction between surface morphology and cells, but also potentially pave the way for efficient and cost-effective design of advanced biomaterial surfaces for better osseointegration. |
| format | Article |
| id | doaj-art-7cd3813da16547e9984b767d86f245f9 |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-7cd3813da16547e9984b767d86f245f92025-01-17T04:52:04ZengElsevierMaterials Today Bio2590-00642025-02-0130101392Construction and high-throughput screening of gradient nanowire coatings on titanium surface towards ameliorated osseointegrationRuiyue Hang0Yuyu Zhao1Huanming Chen2Xiaomei Li3Runhua Yao4Yonghua Sun5Xiaohong Yao6Long Bai7Huaiyu Wang8Yong Han9Ruiqiang Hang10Shanxi Key Laboratory of Biomedical Metal Materials, College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, ChinaShanxi Key Laboratory of Biomedical Metal Materials, College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, ChinaShanxi Key Laboratory of Biomedical Metal Materials, College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, ChinaShanxi Provincial Key Laboratory of Protein Structure Determination, Shanxi Academy of Advanced Research and Innovation, Taiyuan, 030012, ChinaShanxi Key Laboratory of Biomedical Metal Materials, College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, ChinaShanxi Key Laboratory of Biomedical Metal Materials, College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, ChinaShanxi Key Laboratory of Biomedical Metal Materials, College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, China; Corresponding author.Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China; Corresponding author.Center for Human Tissues and Organs Degeneration, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, ChinaState-Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an, 710049, China; Corresponding author.Shanxi Key Laboratory of Biomedical Metal Materials, College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, China; State-Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an, 710049, China; Corresponding author. Shanxi Key Laboratory of Biomedical Metal Materials, College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, China.Surface nano-modification has emerged as an effective strategy to enhance osseointegration of titanium (Ti) implants. Despite its promise, rational optimization of surface nanomorphology for ameliorated osseointegration remains a significant challenge. Our research pioneering developed a one-step alkali etching technique to produce a gradient nanowire coating with continuously varied dimensions on Ti surfaces, which was subsequently served as a versatile platform for high-throughput screening of optimal dimensions to enhance osseointegration. The results showed that macrophages (MФs) that mainly governed the initial inflammatory reaction exhibited a polarization tendency towards pro-healing M2 phenotype with decreased nanowire dimension due to nanomorphology-mediated focal adhesion formation and activation of its downstream signaling pathways (typically PI3K-Akt). Simultaneously, small-sized nanowires with diameter of 5.63–14.25 nm and inter-spacing of 29.42–57.97 nm were conductive to angiogenesis of endothelial cells (ECs) and osteogenesis of bone marrow mesenchymal stem cells (BMSCs), which may share similar mechanisms of MФs. The in vivo results well corroborated these in vitro observations. The knowledge gained from the present work not only advance our understanding of the interaction between surface morphology and cells, but also potentially pave the way for efficient and cost-effective design of advanced biomaterial surfaces for better osseointegration.http://www.sciencedirect.com/science/article/pii/S2590006424004538High-throughput screeningTitanium implantGradient nanowire coatingOsseointegration |
| spellingShingle | Ruiyue Hang Yuyu Zhao Huanming Chen Xiaomei Li Runhua Yao Yonghua Sun Xiaohong Yao Long Bai Huaiyu Wang Yong Han Ruiqiang Hang Construction and high-throughput screening of gradient nanowire coatings on titanium surface towards ameliorated osseointegration Materials Today Bio High-throughput screening Titanium implant Gradient nanowire coating Osseointegration |
| title | Construction and high-throughput screening of gradient nanowire coatings on titanium surface towards ameliorated osseointegration |
| title_full | Construction and high-throughput screening of gradient nanowire coatings on titanium surface towards ameliorated osseointegration |
| title_fullStr | Construction and high-throughput screening of gradient nanowire coatings on titanium surface towards ameliorated osseointegration |
| title_full_unstemmed | Construction and high-throughput screening of gradient nanowire coatings on titanium surface towards ameliorated osseointegration |
| title_short | Construction and high-throughput screening of gradient nanowire coatings on titanium surface towards ameliorated osseointegration |
| title_sort | construction and high throughput screening of gradient nanowire coatings on titanium surface towards ameliorated osseointegration |
| topic | High-throughput screening Titanium implant Gradient nanowire coating Osseointegration |
| url | http://www.sciencedirect.com/science/article/pii/S2590006424004538 |
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