Buprenorphine Alters Inflammatory and Oxidative Stress Molecular Markers in Arthritis
Buprenorphine is recommended for use as an analgesic in animal models including in murine models of collagen-induced arthritis (CIA). However, the effect of buprenorphine on the expression of disease-associated biomarkers is not well defined. We examined the effect of buprenorphine administration on...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/2515408 |
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| author | Mahadevappa Hemshekhar Vidyanand Anaparti Carol Hitchon Neeloffer Mookherjee |
| author_facet | Mahadevappa Hemshekhar Vidyanand Anaparti Carol Hitchon Neeloffer Mookherjee |
| author_sort | Mahadevappa Hemshekhar |
| collection | DOAJ |
| description | Buprenorphine is recommended for use as an analgesic in animal models including in murine models of collagen-induced arthritis (CIA). However, the effect of buprenorphine on the expression of disease-associated biomarkers is not well defined. We examined the effect of buprenorphine administration on disease progression and the expression of inflammatory and oxidative stress markers, in a murine model of CIA. Buprenorphine administration altered the expression of cytokines, IFN-γ, IL-6, and MMP-3, and oxidative markers, for example, iNOS, superoxide dismutase (SOD1), and catalase (CAT), in the CIA mice. As buprenorphine is an analgesic, we further monitored the association of expression of these biomarkers with pain scores in a human cohort of early rheumatoid arthritis (RA). Serum MMP-3 levels and blood mRNA expression of antioxidants sod1 and cat correlated with pain scores in the RA cohort. We have demonstrated that administration of buprenorphine alters the expression of inflammatory and oxidative stress-related molecular markers in a murine model of CIA. This caveat needs to be considered in animal experiments using buprenorphine as an analgesic, as it can be a confounding factor in murine studies used for prediction of response to therapy. Furthermore, the antioxidant enzymes that showed an association with pain scores in the human cohort may be explored as biomarkers for pain in future studies. |
| format | Article |
| id | doaj-art-7ccc0960fddd4693b8668cc314bb89af |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-7ccc0960fddd4693b8668cc314bb89af2025-08-20T03:35:29ZengWileyMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/25154082515408Buprenorphine Alters Inflammatory and Oxidative Stress Molecular Markers in ArthritisMahadevappa Hemshekhar0Vidyanand Anaparti1Carol Hitchon2Neeloffer Mookherjee3Manitoba Centre for Proteomics and Systems Biology, University of Manitoba, Winnipeg, MB, CanadaManitoba Centre for Proteomics and Systems Biology, University of Manitoba, Winnipeg, MB, CanadaDepartment of Internal Medicine, University of Manitoba, Winnipeg, MB, CanadaManitoba Centre for Proteomics and Systems Biology, University of Manitoba, Winnipeg, MB, CanadaBuprenorphine is recommended for use as an analgesic in animal models including in murine models of collagen-induced arthritis (CIA). However, the effect of buprenorphine on the expression of disease-associated biomarkers is not well defined. We examined the effect of buprenorphine administration on disease progression and the expression of inflammatory and oxidative stress markers, in a murine model of CIA. Buprenorphine administration altered the expression of cytokines, IFN-γ, IL-6, and MMP-3, and oxidative markers, for example, iNOS, superoxide dismutase (SOD1), and catalase (CAT), in the CIA mice. As buprenorphine is an analgesic, we further monitored the association of expression of these biomarkers with pain scores in a human cohort of early rheumatoid arthritis (RA). Serum MMP-3 levels and blood mRNA expression of antioxidants sod1 and cat correlated with pain scores in the RA cohort. We have demonstrated that administration of buprenorphine alters the expression of inflammatory and oxidative stress-related molecular markers in a murine model of CIA. This caveat needs to be considered in animal experiments using buprenorphine as an analgesic, as it can be a confounding factor in murine studies used for prediction of response to therapy. Furthermore, the antioxidant enzymes that showed an association with pain scores in the human cohort may be explored as biomarkers for pain in future studies.http://dx.doi.org/10.1155/2017/2515408 |
| spellingShingle | Mahadevappa Hemshekhar Vidyanand Anaparti Carol Hitchon Neeloffer Mookherjee Buprenorphine Alters Inflammatory and Oxidative Stress Molecular Markers in Arthritis Mediators of Inflammation |
| title | Buprenorphine Alters Inflammatory and Oxidative Stress Molecular Markers in Arthritis |
| title_full | Buprenorphine Alters Inflammatory and Oxidative Stress Molecular Markers in Arthritis |
| title_fullStr | Buprenorphine Alters Inflammatory and Oxidative Stress Molecular Markers in Arthritis |
| title_full_unstemmed | Buprenorphine Alters Inflammatory and Oxidative Stress Molecular Markers in Arthritis |
| title_short | Buprenorphine Alters Inflammatory and Oxidative Stress Molecular Markers in Arthritis |
| title_sort | buprenorphine alters inflammatory and oxidative stress molecular markers in arthritis |
| url | http://dx.doi.org/10.1155/2017/2515408 |
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