The Effect of Exercise Intensity on Pancreatic and Liver GLUT-2 Expression in High Fructose-Fed Mice
Excessive high fructose corn syrup (HFCS) leads to metabolic disorders characterized by decreased expression of pancreatic GLUT-2 and increased expression of liver GLUT-2. Exercise is reported to be a non-pharmacological therapy to improve metabolic disorders. This study aims to compare differences...
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FEADEF
2024-08-01
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| Series: | Retos: Nuevas Tendencias en Educación Física, Deportes y Recreación |
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| Online Access: | https://recyt.fecyt.es/index.php/retos/article/view/104464 |
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| author | Tantia Dewi Harianto Bagas Trio Pamungkas Purwo Sri Rejeki Citrawati Dyah Kencono Wungu Joni Susanto Nabilah Izzatunnisa Tri Hartini Yuliawati Shariff Halim Adi Pranoto |
| author_facet | Tantia Dewi Harianto Bagas Trio Pamungkas Purwo Sri Rejeki Citrawati Dyah Kencono Wungu Joni Susanto Nabilah Izzatunnisa Tri Hartini Yuliawati Shariff Halim Adi Pranoto |
| author_sort | Tantia Dewi Harianto |
| collection | DOAJ |
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Excessive high fructose corn syrup (HFCS) leads to metabolic disorders characterized by decreased expression of pancreatic GLUT-2 and increased expression of liver GLUT-2. Exercise is reported to be a non-pharmacological therapy to improve metabolic disorders. This study aims to compare differences in exercise intensity to changes in pancreatic and liver GLUT-2 expression in mice induced by high fructose. A total of 36 male mice (Mus musculus), weighing 20-30 grams, 8-week-old, were randomly divided into 4 groups: C (Control; n = 9), G1 (light intensity swimming exercises; n = 9), G2 (moderate intensity swimming exercises; n = 9), and G3 (heavy intensity swimming exercises; n = 9). All groups were given 30% fructose solution orally (oral ad libitum) for 8 weeks. Meanwhile, swimming exercise was given out 3×/week for 8 weeks with three different intensities. Pancreatic and liver GLUT-2 expression was measured using immunohistochemistry (IHC) and the results of pancreatic and liver GLUT-2 expression measurements were evaluated using the Immunoreactive Score (IRS). The analysis of body weight using one-way ANOVA followed by Tukey's Honest Significant Difference (HSD) post hoc test indicates that G2 significantly reduces weight compared to C, G1, and G3 (p ≤ 0.05). Statistical analysis was done using the Kruskal-Wallis non-parametric test and followed up with the Mann-Whitney U Test with a significant level of 5%. The data showed that G2 significantly increased expression of pancreatic GLUT-2 and decreased expression of liver GLUT-2 compared to C, G1, and G3 (p ≤ 0.05). In conclusion, moderate-intensity exercise has the most optimal effect in increasing the expression of pancreatic GLUT-2 and decreasing the expression of liver GLUT-2.
Keywords: Obesity, Insulin resistance, GLUT-2 expression, Exercise, High fructose
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| format | Article |
| id | doaj-art-7ccbd07b4a994832adb6286e9ce649bf |
| institution | OA Journals |
| issn | 1579-1726 1988-2041 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | FEADEF |
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| series | Retos: Nuevas Tendencias en Educación Física, Deportes y Recreación |
| spelling | doaj-art-7ccbd07b4a994832adb6286e9ce649bf2025-08-20T02:35:18ZengFEADEFRetos: Nuevas Tendencias en Educación Física, Deportes y Recreación1579-17261988-20412024-08-015710.47197/retos.v57.104464The Effect of Exercise Intensity on Pancreatic and Liver GLUT-2 Expression in High Fructose-Fed MiceTantia Dewi Harianto0Bagas Trio Pamungkas1Purwo Sri Rejeki2Citrawati Dyah Kencono Wungu3Joni Susanto4Nabilah Izzatunnisa5Tri Hartini Yuliawati6Shariff Halim7Adi Pranoto8Sport Health Science, Faculty of Medicine, Universitas AirlanggaMedical Program, Faculty of Medicine, Universitas AirlanggaDepartment of Medical Physiology and Biochemistry, Faculty of Medicine, Universitas AirlanggaBiochemistry Division, Department of Medical Physiology and Biochemistry, Faculty of Medicine, Universitas AirlanggaDepartment of Anatomy, Histology & Pharmacology, Faculty of Medicine, Universitas AirlanggaMedical Program, Faculty of Medicine, Universitas AirlanggaDepartment of Anatomy, Histology & Pharmacology, Faculty of Medicine, Universitas AirlanggaFaculty of Health Sciences, University Technology MARA (UiTM) Pulau PinangDoctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga Excessive high fructose corn syrup (HFCS) leads to metabolic disorders characterized by decreased expression of pancreatic GLUT-2 and increased expression of liver GLUT-2. Exercise is reported to be a non-pharmacological therapy to improve metabolic disorders. This study aims to compare differences in exercise intensity to changes in pancreatic and liver GLUT-2 expression in mice induced by high fructose. A total of 36 male mice (Mus musculus), weighing 20-30 grams, 8-week-old, were randomly divided into 4 groups: C (Control; n = 9), G1 (light intensity swimming exercises; n = 9), G2 (moderate intensity swimming exercises; n = 9), and G3 (heavy intensity swimming exercises; n = 9). All groups were given 30% fructose solution orally (oral ad libitum) for 8 weeks. Meanwhile, swimming exercise was given out 3×/week for 8 weeks with three different intensities. Pancreatic and liver GLUT-2 expression was measured using immunohistochemistry (IHC) and the results of pancreatic and liver GLUT-2 expression measurements were evaluated using the Immunoreactive Score (IRS). The analysis of body weight using one-way ANOVA followed by Tukey's Honest Significant Difference (HSD) post hoc test indicates that G2 significantly reduces weight compared to C, G1, and G3 (p ≤ 0.05). Statistical analysis was done using the Kruskal-Wallis non-parametric test and followed up with the Mann-Whitney U Test with a significant level of 5%. The data showed that G2 significantly increased expression of pancreatic GLUT-2 and decreased expression of liver GLUT-2 compared to C, G1, and G3 (p ≤ 0.05). In conclusion, moderate-intensity exercise has the most optimal effect in increasing the expression of pancreatic GLUT-2 and decreasing the expression of liver GLUT-2. Keywords: Obesity, Insulin resistance, GLUT-2 expression, Exercise, High fructose https://recyt.fecyt.es/index.php/retos/article/view/104464ObesityInsulin resistanceGLUT-2 expressionExerciseHigh fructose |
| spellingShingle | Tantia Dewi Harianto Bagas Trio Pamungkas Purwo Sri Rejeki Citrawati Dyah Kencono Wungu Joni Susanto Nabilah Izzatunnisa Tri Hartini Yuliawati Shariff Halim Adi Pranoto The Effect of Exercise Intensity on Pancreatic and Liver GLUT-2 Expression in High Fructose-Fed Mice Retos: Nuevas Tendencias en Educación Física, Deportes y Recreación Obesity Insulin resistance GLUT-2 expression Exercise High fructose |
| title | The Effect of Exercise Intensity on Pancreatic and Liver GLUT-2 Expression in High Fructose-Fed Mice |
| title_full | The Effect of Exercise Intensity on Pancreatic and Liver GLUT-2 Expression in High Fructose-Fed Mice |
| title_fullStr | The Effect of Exercise Intensity on Pancreatic and Liver GLUT-2 Expression in High Fructose-Fed Mice |
| title_full_unstemmed | The Effect of Exercise Intensity on Pancreatic and Liver GLUT-2 Expression in High Fructose-Fed Mice |
| title_short | The Effect of Exercise Intensity on Pancreatic and Liver GLUT-2 Expression in High Fructose-Fed Mice |
| title_sort | effect of exercise intensity on pancreatic and liver glut 2 expression in high fructose fed mice |
| topic | Obesity Insulin resistance GLUT-2 expression Exercise High fructose |
| url | https://recyt.fecyt.es/index.php/retos/article/view/104464 |
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