Transcriptome Proffling, Physiological and Biochemical Analyses Reveal Comprehensive Insights into Cadmium Stress in <i>Myricaria laxiflora</i>
With the expansion of cities and the development of industries, heavy metal pollution has caused a serious negative impact on the growth and development of animals and plants, which has become a global economic and social problem. Cadmium (Cd) is one of the main heavy metals that threaten the growth...
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2024-12-01
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| author | Yang Peng Yu-Bing Yang Jing-Cheng Wang Mao-Yuan Tian Xing-Hai Yuan Zhi-Jiang Yang You-Wei Zuo Hong-Ping Deng |
| author_facet | Yang Peng Yu-Bing Yang Jing-Cheng Wang Mao-Yuan Tian Xing-Hai Yuan Zhi-Jiang Yang You-Wei Zuo Hong-Ping Deng |
| author_sort | Yang Peng |
| collection | DOAJ |
| description | With the expansion of cities and the development of industries, heavy metal pollution has caused a serious negative impact on the growth and development of animals and plants, which has become a global economic and social problem. Cadmium (Cd) is one of the main heavy metals that threaten the growth and development of plants, and it can lead to the imminent extinction of plants in severe cases. The part of upper reaches of the Yangtze River in China from Yibin to the Three Gorges Reservoir has been contaminated with varying degrees of Cd, and a rare and endangered plant called <i>Myricaria laxiflora</i> also lives in this area. The stress of heavy metal Cd on <i>M. laxiflora</i> populations is still unknown. In this study, we used the seedlings of <i>M. laxiflora</i> as materials, and adopted conventional physiological and biochemical analyses to characterize the morphological and physiological responses of <i>M. laxiflora</i> under different concentrations of Cd, and analyzed its response to Cd stress at the transcriptional level. The results showed that the wild population of <i>M. laxiflora</i> was stressed by the heavy metal Cd. High concentrations of Cd can inhibit the growth of <i>M. laxiflora. M. laxiflora</i> responded to the Cd stress through resistance substances such as malondialdehyde (MDA), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), superoxide dismutase (SOD), catalase (CAT), and phytohormones such as auxin (IAA), gibberellin (GA) and abscisic acid (ABA). Transcriptome analysis was carried out on <i>M. lasiflora</i> seedlings exposed to 24 h, 48 h, and 72 h of Cd stress. Compared with 0 h (control), 2470, 11,707, and 11,733 differential expressed genes (DEGs) were identified, respectively. Among them, the number of down-regulated genes is more than the number of up-regulated genes. Transcriptome analysis showed that the upregulated genes were mainly enriched in MAPK signaling pathway, ethylene-induced pathway, ABA response pathway and other pathways, and the downregulated genes were mainly enriched in photosynthesis related pathways. Cd stress affected photosynthesis of <i>M. laxiflora</i>, and <i>M. laxiflora</i> may activate the MAPK signaling pathway through ethylene and ABA to improve the ability of Cd stress tolerance. These results reveal morphological changes, physiological and biochemical reactions and related key response pathways of <i>M. laxiflora</i> during Cd stress. It can provide a reference basis for habitat restoration and selection of wildlife environments for <i>M. laxiflora</i>. |
| format | Article |
| id | doaj-art-7cc812e8740544e1b8cd83ed42c35e48 |
| institution | Kabale University |
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| publishDate | 2024-12-01 |
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| spelling | doaj-art-7cc812e8740544e1b8cd83ed42c35e482024-12-13T16:30:24ZengMDPI AGPlants2223-77472024-12-011323343310.3390/plants13233433Transcriptome Proffling, Physiological and Biochemical Analyses Reveal Comprehensive Insights into Cadmium Stress in <i>Myricaria laxiflora</i>Yang Peng0Yu-Bing Yang1Jing-Cheng Wang2Mao-Yuan Tian3Xing-Hai Yuan4Zhi-Jiang Yang5You-Wei Zuo6Hong-Ping Deng7Key Laboratory of Eco-Environment in the Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, ChinaKey Laboratory of Eco-Environment in the Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, ChinaKey Laboratory of Eco-Environment in the Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, ChinaJiangjin Forestry Bureau, Chongqing 402260, ChinaJiangjin Forestry Bureau, Chongqing 402260, ChinaKey Laboratory of Eco-Environment in the Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, ChinaKey Laboratory of Eco-Environment in the Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, ChinaKey Laboratory of Eco-Environment in the Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, ChinaWith the expansion of cities and the development of industries, heavy metal pollution has caused a serious negative impact on the growth and development of animals and plants, which has become a global economic and social problem. Cadmium (Cd) is one of the main heavy metals that threaten the growth and development of plants, and it can lead to the imminent extinction of plants in severe cases. The part of upper reaches of the Yangtze River in China from Yibin to the Three Gorges Reservoir has been contaminated with varying degrees of Cd, and a rare and endangered plant called <i>Myricaria laxiflora</i> also lives in this area. The stress of heavy metal Cd on <i>M. laxiflora</i> populations is still unknown. In this study, we used the seedlings of <i>M. laxiflora</i> as materials, and adopted conventional physiological and biochemical analyses to characterize the morphological and physiological responses of <i>M. laxiflora</i> under different concentrations of Cd, and analyzed its response to Cd stress at the transcriptional level. The results showed that the wild population of <i>M. laxiflora</i> was stressed by the heavy metal Cd. High concentrations of Cd can inhibit the growth of <i>M. laxiflora. M. laxiflora</i> responded to the Cd stress through resistance substances such as malondialdehyde (MDA), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), superoxide dismutase (SOD), catalase (CAT), and phytohormones such as auxin (IAA), gibberellin (GA) and abscisic acid (ABA). Transcriptome analysis was carried out on <i>M. lasiflora</i> seedlings exposed to 24 h, 48 h, and 72 h of Cd stress. Compared with 0 h (control), 2470, 11,707, and 11,733 differential expressed genes (DEGs) were identified, respectively. Among them, the number of down-regulated genes is more than the number of up-regulated genes. Transcriptome analysis showed that the upregulated genes were mainly enriched in MAPK signaling pathway, ethylene-induced pathway, ABA response pathway and other pathways, and the downregulated genes were mainly enriched in photosynthesis related pathways. Cd stress affected photosynthesis of <i>M. laxiflora</i>, and <i>M. laxiflora</i> may activate the MAPK signaling pathway through ethylene and ABA to improve the ability of Cd stress tolerance. These results reveal morphological changes, physiological and biochemical reactions and related key response pathways of <i>M. laxiflora</i> during Cd stress. It can provide a reference basis for habitat restoration and selection of wildlife environments for <i>M. laxiflora</i>.https://www.mdpi.com/2223-7747/13/23/3433<i>M. laxiflora</i>cadmium stressmorphological responsephysiological and biochemical responsedifferentially expressed genes |
| spellingShingle | Yang Peng Yu-Bing Yang Jing-Cheng Wang Mao-Yuan Tian Xing-Hai Yuan Zhi-Jiang Yang You-Wei Zuo Hong-Ping Deng Transcriptome Proffling, Physiological and Biochemical Analyses Reveal Comprehensive Insights into Cadmium Stress in <i>Myricaria laxiflora</i> Plants <i>M. laxiflora</i> cadmium stress morphological response physiological and biochemical response differentially expressed genes |
| title | Transcriptome Proffling, Physiological and Biochemical Analyses Reveal Comprehensive Insights into Cadmium Stress in <i>Myricaria laxiflora</i> |
| title_full | Transcriptome Proffling, Physiological and Biochemical Analyses Reveal Comprehensive Insights into Cadmium Stress in <i>Myricaria laxiflora</i> |
| title_fullStr | Transcriptome Proffling, Physiological and Biochemical Analyses Reveal Comprehensive Insights into Cadmium Stress in <i>Myricaria laxiflora</i> |
| title_full_unstemmed | Transcriptome Proffling, Physiological and Biochemical Analyses Reveal Comprehensive Insights into Cadmium Stress in <i>Myricaria laxiflora</i> |
| title_short | Transcriptome Proffling, Physiological and Biochemical Analyses Reveal Comprehensive Insights into Cadmium Stress in <i>Myricaria laxiflora</i> |
| title_sort | transcriptome proffling physiological and biochemical analyses reveal comprehensive insights into cadmium stress in i myricaria laxiflora i |
| topic | <i>M. laxiflora</i> cadmium stress morphological response physiological and biochemical response differentially expressed genes |
| url | https://www.mdpi.com/2223-7747/13/23/3433 |
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