An Enhanced Bimetallic Optical Fiber SPR Biosensor Using Graphene Oxide for the Label-Free and Sensitive Detection of Human IgG
A fiber-reinforced SPR sensor based on silver-nucleated gold-shell bimetallic nanoparticles and graphene oxide was developed and applied to human IgG detection. The refractive index (RI) sensitivity of the Ag@Au/GO fiber SPR sensor is as high as 4715.9 nm/RIU in the RI range of 1.333–1.365. Staphylo...
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MDPI AG
2025-03-01
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| author | Qiang Xu Huiting Yin Mei Cui Renliang Huang Rongxin Su |
| author_facet | Qiang Xu Huiting Yin Mei Cui Renliang Huang Rongxin Su |
| author_sort | Qiang Xu |
| collection | DOAJ |
| description | A fiber-reinforced SPR sensor based on silver-nucleated gold-shell bimetallic nanoparticles and graphene oxide was developed and applied to human IgG detection. The refractive index (RI) sensitivity of the Ag@Au/GO fiber SPR sensor is as high as 4715.9 nm/RIU in the RI range of 1.333–1.365. Staphylococcus aureus protein A (SPA) can specifically recognize and bind to the fragment crystallizable (Fc) of the antibody; it facilitates the highly targeted immobilization of the antibody. SPA and rabbit anti-human IgG were immobilized on the surface of the Ag@Au/GO fiber SPR sensor for the detection of different concentrations of human IgG with a sensitivity of 0.53 nm/μg/mL and detection limits of 0.037 μg/mL. This biosensor based on the mixed structure of GO and Ag@Au combined the common advantages of the two materials. Therefore, our study provides a simple platform for biological analysis and has a good application prospect. |
| format | Article |
| id | doaj-art-7cc5ef486a4546c98eb39f720cec2bae |
| institution | OA Journals |
| issn | 1424-8220 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
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| series | Sensors |
| spelling | doaj-art-7cc5ef486a4546c98eb39f720cec2bae2025-08-20T02:06:15ZengMDPI AGSensors1424-82202025-03-01255163010.3390/s25051630An Enhanced Bimetallic Optical Fiber SPR Biosensor Using Graphene Oxide for the Label-Free and Sensitive Detection of Human IgGQiang Xu0Huiting Yin1Mei Cui2Renliang Huang3Rongxin Su4Tianjin Key Laboratory for Marine Environmental Research and Service, School of Marine Science and Technology, Tianjin University, Tianjin 300072, ChinaNingbo Key Laboratory of Green Petrochemical Carbon Emission Reduction Technology and Equipment, Zhejiang Institute of Tianjin University, Ningbo 315201, ChinaState Key Laboratory of Chemical Engineering, Tianjin Key Laboratory of Membrane Science and Desalination Technology, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, ChinaTianjin Key Laboratory for Marine Environmental Research and Service, School of Marine Science and Technology, Tianjin University, Tianjin 300072, ChinaTianjin Key Laboratory for Marine Environmental Research and Service, School of Marine Science and Technology, Tianjin University, Tianjin 300072, ChinaA fiber-reinforced SPR sensor based on silver-nucleated gold-shell bimetallic nanoparticles and graphene oxide was developed and applied to human IgG detection. The refractive index (RI) sensitivity of the Ag@Au/GO fiber SPR sensor is as high as 4715.9 nm/RIU in the RI range of 1.333–1.365. Staphylococcus aureus protein A (SPA) can specifically recognize and bind to the fragment crystallizable (Fc) of the antibody; it facilitates the highly targeted immobilization of the antibody. SPA and rabbit anti-human IgG were immobilized on the surface of the Ag@Au/GO fiber SPR sensor for the detection of different concentrations of human IgG with a sensitivity of 0.53 nm/μg/mL and detection limits of 0.037 μg/mL. This biosensor based on the mixed structure of GO and Ag@Au combined the common advantages of the two materials. Therefore, our study provides a simple platform for biological analysis and has a good application prospect.https://www.mdpi.com/1424-8220/25/5/1630bimetal sensorfiber-optic SPRgraphene oxideantibodyhuman IgG |
| spellingShingle | Qiang Xu Huiting Yin Mei Cui Renliang Huang Rongxin Su An Enhanced Bimetallic Optical Fiber SPR Biosensor Using Graphene Oxide for the Label-Free and Sensitive Detection of Human IgG Sensors bimetal sensor fiber-optic SPR graphene oxide antibody human IgG |
| title | An Enhanced Bimetallic Optical Fiber SPR Biosensor Using Graphene Oxide for the Label-Free and Sensitive Detection of Human IgG |
| title_full | An Enhanced Bimetallic Optical Fiber SPR Biosensor Using Graphene Oxide for the Label-Free and Sensitive Detection of Human IgG |
| title_fullStr | An Enhanced Bimetallic Optical Fiber SPR Biosensor Using Graphene Oxide for the Label-Free and Sensitive Detection of Human IgG |
| title_full_unstemmed | An Enhanced Bimetallic Optical Fiber SPR Biosensor Using Graphene Oxide for the Label-Free and Sensitive Detection of Human IgG |
| title_short | An Enhanced Bimetallic Optical Fiber SPR Biosensor Using Graphene Oxide for the Label-Free and Sensitive Detection of Human IgG |
| title_sort | enhanced bimetallic optical fiber spr biosensor using graphene oxide for the label free and sensitive detection of human igg |
| topic | bimetal sensor fiber-optic SPR graphene oxide antibody human IgG |
| url | https://www.mdpi.com/1424-8220/25/5/1630 |
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