Extrachromosomal circular DNA as a novel biomarker for the progression of colorectal cancer
Abstract Background Extrachromosomal circular DNA (eccDNA) has potential in tumor diagnosis, particularly for improving diagnostic accuracy and early cancer detection; however, many challenges remain in its application to clinical practice. Methods We conducted a Circle‐Seq analysis on clinical samp...
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| Format: | Article |
| Language: | English |
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BMC
2025-04-01
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| Series: | Molecular Medicine |
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| Online Access: | https://doi.org/10.1186/s10020-025-01164-y |
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| author | Quanpeng Qiu Yi Ding Xiaolong Guo Jing Han Jiaqi Zhang Yaping Liu Junjun She Yinnan Chen |
| author_facet | Quanpeng Qiu Yi Ding Xiaolong Guo Jing Han Jiaqi Zhang Yaping Liu Junjun She Yinnan Chen |
| author_sort | Quanpeng Qiu |
| collection | DOAJ |
| description | Abstract Background Extrachromosomal circular DNA (eccDNA) has potential in tumor diagnosis, particularly for improving diagnostic accuracy and early cancer detection; however, many challenges remain in its application to clinical practice. Methods We conducted a Circle‐Seq analysis on clinical samples at different stages of colorectal cancer progression to examine the dynamic changes of eccDNA during the progression of colorectal cancer. We used breakpoint-specific PCR to verify candidate eccDNAs identified by Circle‐Seq. The results were further validated using the AOM/DSS-induced colorectal cancer model. Results There was an increase in the abundance of eccDNA with the progression of colorectal cancer. The genes associated with these eccDNA molecules were primarily related to signaling pathways involved in tumor development and metastasis. Our analysis also revealed that eccDNA abundance positively correlates with gene expression, and eccDNA derived from specific genes has potential value for the early diagnosis of tumors. Conclusions This study revealed a connection between eccDNA and colorectal cancer progression and highlights the clinical potential of eccDNA for the early diagnosis of colorectal cancer. |
| format | Article |
| id | doaj-art-7cb67e4997754ce796c4462bc171f11d |
| institution | OA Journals |
| issn | 1528-3658 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Medicine |
| spelling | doaj-art-7cb67e4997754ce796c4462bc171f11d2025-08-20T01:54:30ZengBMCMolecular Medicine1528-36582025-04-0131111110.1186/s10020-025-01164-yExtrachromosomal circular DNA as a novel biomarker for the progression of colorectal cancerQuanpeng Qiu0Yi Ding1Xiaolong Guo2Jing Han3Jiaqi Zhang4Yaping Liu5Junjun She6Yinnan Chen7Department of General Surgery, The First Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of General Surgery, The First Affiliated Hospital of Xi’an Jiaotong UniversityCenter for Gut Microbiome Research, Med-X Institute, the First Affiliated Hospital of Xi’an Jiao Tong UniversityCenter for Gut Microbiome Research, Med-X Institute, the First Affiliated Hospital of Xi’an Jiao Tong UniversityCenter for Gut Microbiome Research, Med-X Institute, the First Affiliated Hospital of Xi’an Jiao Tong UniversityDepartment of High Talent, The First Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of General Surgery, The First Affiliated Hospital of Xi’an Jiaotong UniversityCenter for Gut Microbiome Research, Med-X Institute, the First Affiliated Hospital of Xi’an Jiao Tong UniversityAbstract Background Extrachromosomal circular DNA (eccDNA) has potential in tumor diagnosis, particularly for improving diagnostic accuracy and early cancer detection; however, many challenges remain in its application to clinical practice. Methods We conducted a Circle‐Seq analysis on clinical samples at different stages of colorectal cancer progression to examine the dynamic changes of eccDNA during the progression of colorectal cancer. We used breakpoint-specific PCR to verify candidate eccDNAs identified by Circle‐Seq. The results were further validated using the AOM/DSS-induced colorectal cancer model. Results There was an increase in the abundance of eccDNA with the progression of colorectal cancer. The genes associated with these eccDNA molecules were primarily related to signaling pathways involved in tumor development and metastasis. Our analysis also revealed that eccDNA abundance positively correlates with gene expression, and eccDNA derived from specific genes has potential value for the early diagnosis of tumors. Conclusions This study revealed a connection between eccDNA and colorectal cancer progression and highlights the clinical potential of eccDNA for the early diagnosis of colorectal cancer.https://doi.org/10.1186/s10020-025-01164-yColorectal cancerExtrachromosomal circular DNABiomarkers |
| spellingShingle | Quanpeng Qiu Yi Ding Xiaolong Guo Jing Han Jiaqi Zhang Yaping Liu Junjun She Yinnan Chen Extrachromosomal circular DNA as a novel biomarker for the progression of colorectal cancer Molecular Medicine Colorectal cancer Extrachromosomal circular DNA Biomarkers |
| title | Extrachromosomal circular DNA as a novel biomarker for the progression of colorectal cancer |
| title_full | Extrachromosomal circular DNA as a novel biomarker for the progression of colorectal cancer |
| title_fullStr | Extrachromosomal circular DNA as a novel biomarker for the progression of colorectal cancer |
| title_full_unstemmed | Extrachromosomal circular DNA as a novel biomarker for the progression of colorectal cancer |
| title_short | Extrachromosomal circular DNA as a novel biomarker for the progression of colorectal cancer |
| title_sort | extrachromosomal circular dna as a novel biomarker for the progression of colorectal cancer |
| topic | Colorectal cancer Extrachromosomal circular DNA Biomarkers |
| url | https://doi.org/10.1186/s10020-025-01164-y |
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