Hepatic and Renal Toxicity Induced by TiO2 Nanoparticles in Rats: A Morphological and Metabonomic Study
Titanium dioxide (TiO2) nanoparticles (NPs) are produced abundantly and are frequently used as a white pigment in the manufacture of paints, foods, paper, and toothpaste. Despite the wide ranges of uses, there is a lack of information on the impact of NPs on animal and human health. In the present s...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2019/5767012 |
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| author | Xavier Valentini Pascaline Rugira Annica Frau Vanessa Tagliatti Raphaël Conotte Sophie Laurent Jean-Marie Colet Denis Nonclercq |
| author_facet | Xavier Valentini Pascaline Rugira Annica Frau Vanessa Tagliatti Raphaël Conotte Sophie Laurent Jean-Marie Colet Denis Nonclercq |
| author_sort | Xavier Valentini |
| collection | DOAJ |
| description | Titanium dioxide (TiO2) nanoparticles (NPs) are produced abundantly and are frequently used as a white pigment in the manufacture of paints, foods, paper, and toothpaste. Despite the wide ranges of uses, there is a lack of information on the impact of NPs on animal and human health. In the present study, rats were exposed to different doses of TiO2 nanoparticles and sacrificed, respectively, 4 days, 1 month, and 2 months after treatment. Dosage of TiO2 in tissues was performed by ICP-AES and revealed an important accumulation of TiO2 in the liver. The nanoparticles induced morphological and physiological alterations in liver and kidney. In the liver, these alterations mainly affect the hepatocytes located around the centrilobular veins. These cells were the site of an oxidative stress evidenced by immunocytochemical detection of 4-hydroxynonenal (4-HNE). Kupffer cells are also the site of an important oxidative stress following the massive internalization of TiO2 nanoparticles. Enzymatic markers of liver and kidney functions (such as AST and uric acid) are also disrupted only in animals exposed to highest doses. The metabonomic approach allowed us to detect modifications in urine samples already detectable after 4 days in animals treated at the lowest dose. This metabonomic pattern testifies an oxidative stress as well as renal and hepatic alterations. |
| format | Article |
| id | doaj-art-7caf39e249f2482b887eda5c32e2e339 |
| institution | OA Journals |
| issn | 1687-8191 1687-8205 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-7caf39e249f2482b887eda5c32e2e3392025-08-20T02:07:24ZengWileyJournal of Toxicology1687-81911687-82052019-01-01201910.1155/2019/57670125767012Hepatic and Renal Toxicity Induced by TiO2 Nanoparticles in Rats: A Morphological and Metabonomic StudyXavier Valentini0Pascaline Rugira1Annica Frau2Vanessa Tagliatti3Raphaël Conotte4Sophie Laurent5Jean-Marie Colet6Denis Nonclercq7Laboratory of Histology, University of Mons, Institute for Health Sciences and Technology, Faculty of Medicine and Pharmacy, 23 Place du Parc, 7000 Mons, BelgiumLaboratory of Histology, University of Mons, Institute for Health Sciences and Technology, Faculty of Medicine and Pharmacy, 23 Place du Parc, 7000 Mons, BelgiumLaboratory of Histology, University of Mons, Institute for Health Sciences and Technology, Faculty of Medicine and Pharmacy, 23 Place du Parc, 7000 Mons, BelgiumLaboratory of Human Biology & Toxicology, University of Mons, Institute for Health Sciences and Technology, Faculty of Medicine and Pharmacy, 23 Place du Parc, 7000 Mons, BelgiumLaboratory of Human Biology & Toxicology, University of Mons, Institute for Health Sciences and Technology, Faculty of Medicine and Pharmacy, 23 Place du Parc, 7000 Mons, BelgiumLaboratory of General, Organic and Biomedical Chemistry, NMR and Molecular Imaging Laboratory, University of Mons, Institute for Health Sciences and Technology and Biosciences, Faculty of Medicine and Pharmacy, 23 Place du Parc, 7000 Mons, BelgiumLaboratory of Human Biology & Toxicology, University of Mons, Institute for Health Sciences and Technology, Faculty of Medicine and Pharmacy, 23 Place du Parc, 7000 Mons, BelgiumLaboratory of Histology, University of Mons, Institute for Health Sciences and Technology, Faculty of Medicine and Pharmacy, 23 Place du Parc, 7000 Mons, BelgiumTitanium dioxide (TiO2) nanoparticles (NPs) are produced abundantly and are frequently used as a white pigment in the manufacture of paints, foods, paper, and toothpaste. Despite the wide ranges of uses, there is a lack of information on the impact of NPs on animal and human health. In the present study, rats were exposed to different doses of TiO2 nanoparticles and sacrificed, respectively, 4 days, 1 month, and 2 months after treatment. Dosage of TiO2 in tissues was performed by ICP-AES and revealed an important accumulation of TiO2 in the liver. The nanoparticles induced morphological and physiological alterations in liver and kidney. In the liver, these alterations mainly affect the hepatocytes located around the centrilobular veins. These cells were the site of an oxidative stress evidenced by immunocytochemical detection of 4-hydroxynonenal (4-HNE). Kupffer cells are also the site of an important oxidative stress following the massive internalization of TiO2 nanoparticles. Enzymatic markers of liver and kidney functions (such as AST and uric acid) are also disrupted only in animals exposed to highest doses. The metabonomic approach allowed us to detect modifications in urine samples already detectable after 4 days in animals treated at the lowest dose. This metabonomic pattern testifies an oxidative stress as well as renal and hepatic alterations.http://dx.doi.org/10.1155/2019/5767012 |
| spellingShingle | Xavier Valentini Pascaline Rugira Annica Frau Vanessa Tagliatti Raphaël Conotte Sophie Laurent Jean-Marie Colet Denis Nonclercq Hepatic and Renal Toxicity Induced by TiO2 Nanoparticles in Rats: A Morphological and Metabonomic Study Journal of Toxicology |
| title | Hepatic and Renal Toxicity Induced by TiO2 Nanoparticles in Rats: A Morphological and Metabonomic Study |
| title_full | Hepatic and Renal Toxicity Induced by TiO2 Nanoparticles in Rats: A Morphological and Metabonomic Study |
| title_fullStr | Hepatic and Renal Toxicity Induced by TiO2 Nanoparticles in Rats: A Morphological and Metabonomic Study |
| title_full_unstemmed | Hepatic and Renal Toxicity Induced by TiO2 Nanoparticles in Rats: A Morphological and Metabonomic Study |
| title_short | Hepatic and Renal Toxicity Induced by TiO2 Nanoparticles in Rats: A Morphological and Metabonomic Study |
| title_sort | hepatic and renal toxicity induced by tio2 nanoparticles in rats a morphological and metabonomic study |
| url | http://dx.doi.org/10.1155/2019/5767012 |
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