The extraction of effective components and an antioxidant activity study of Tulipa edulis
Plant extracts from natural sources are an excellent choice for food additives and natural antioxidants. In this study, the active components of Tulipa edulis were extracted and analysed, and their antioxidant capacity was measured. Then, the crude extract mixture was separated and purified using a...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2024-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023001611 |
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| author | Doudou Zhang Dong Xiao Tingting Yin Shuangzhi Zhao Olena Zhur Xun Xiao Hailun He Leilei Chen |
| author_facet | Doudou Zhang Dong Xiao Tingting Yin Shuangzhi Zhao Olena Zhur Xun Xiao Hailun He Leilei Chen |
| author_sort | Doudou Zhang |
| collection | DOAJ |
| description | Plant extracts from natural sources are an excellent choice for food additives and natural antioxidants. In this study, the active components of Tulipa edulis were extracted and analysed, and their antioxidant capacity was measured. Then, the crude extract mixture was separated and purified using a Sephadex LH-20 gel, and the antioxidant activity of the purified products was determined. Human umbilical vein endothelial human umbilical vein endothelial cells (HUVEC) cells were treated with 35 mmol/L glucose to construct a model of oxidative stress. Then, the cells were treated with the active component to observe whether the products of T. edulis could have a good protective effect on HUVEC cells induced by glucose. Transcriptome analysis was also performed on HUVEC cells after same treatment to explore the possible mechanism of the component F2 protecting HUVEC cells from oxidative stress induced by high glucose. The results showed that component F2 obtained from T. edulis has strong antioxidant activity. Moreover, F2 can play a strong antioxidant protective role in HUVEC cells. Meanwhile, the gene expression of heme oxygenase 1 (HO-1), γ-glutamyl cysteine ligase catalytic subunit (GCLC) and NAD(P)H quinone oxidoreductase-1 (NQO1) in HUVEC cells was up-regulated after treated with F2. This study provides reference value for the further development and application of T. edulis and the development of functional food. |
| format | Article |
| id | doaj-art-7caeb757ea224a33b906e8ceaae169a3 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-7caeb757ea224a33b906e8ceaae169a32025-02-03T06:57:18ZengTsinghua University PressFood Science and Human Wellness2213-45302024-01-01131276286The extraction of effective components and an antioxidant activity study of Tulipa edulisDoudou Zhang0Dong Xiao1Tingting Yin2Shuangzhi Zhao3Olena Zhur4Xun Xiao5Hailun He6Leilei Chen7Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 102488, China; School of Life Sciences, Central South University, Changsha 410013, ChinaState Key Laboratory of Coal Resources and Safe Mining, University of Mining and Technology, Xuzhou 221116, ChinaSchool of Life Sciences, Central South University, Changsha 410013, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 102488, China; Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaSchool of Life Sciences, Central South University, Changsha 410013, ChinaSchool of Life Sciences, Central South University, Changsha 410013, ChinaSchool of Life Sciences, Central South University, Changsha 410013, China; Corresponding authors.Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 102488, China; Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, China; Corresponding authors.Plant extracts from natural sources are an excellent choice for food additives and natural antioxidants. In this study, the active components of Tulipa edulis were extracted and analysed, and their antioxidant capacity was measured. Then, the crude extract mixture was separated and purified using a Sephadex LH-20 gel, and the antioxidant activity of the purified products was determined. Human umbilical vein endothelial human umbilical vein endothelial cells (HUVEC) cells were treated with 35 mmol/L glucose to construct a model of oxidative stress. Then, the cells were treated with the active component to observe whether the products of T. edulis could have a good protective effect on HUVEC cells induced by glucose. Transcriptome analysis was also performed on HUVEC cells after same treatment to explore the possible mechanism of the component F2 protecting HUVEC cells from oxidative stress induced by high glucose. The results showed that component F2 obtained from T. edulis has strong antioxidant activity. Moreover, F2 can play a strong antioxidant protective role in HUVEC cells. Meanwhile, the gene expression of heme oxygenase 1 (HO-1), γ-glutamyl cysteine ligase catalytic subunit (GCLC) and NAD(P)H quinone oxidoreductase-1 (NQO1) in HUVEC cells was up-regulated after treated with F2. This study provides reference value for the further development and application of T. edulis and the development of functional food.http://www.sciencedirect.com/science/article/pii/S2213453023001611Tulipa edulisOxidative stressPurificationAntioxidant |
| spellingShingle | Doudou Zhang Dong Xiao Tingting Yin Shuangzhi Zhao Olena Zhur Xun Xiao Hailun He Leilei Chen The extraction of effective components and an antioxidant activity study of Tulipa edulis Food Science and Human Wellness Tulipa edulis Oxidative stress Purification Antioxidant |
| title | The extraction of effective components and an antioxidant activity study of Tulipa edulis |
| title_full | The extraction of effective components and an antioxidant activity study of Tulipa edulis |
| title_fullStr | The extraction of effective components and an antioxidant activity study of Tulipa edulis |
| title_full_unstemmed | The extraction of effective components and an antioxidant activity study of Tulipa edulis |
| title_short | The extraction of effective components and an antioxidant activity study of Tulipa edulis |
| title_sort | extraction of effective components and an antioxidant activity study of tulipa edulis |
| topic | Tulipa edulis Oxidative stress Purification Antioxidant |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023001611 |
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