Sequencing airborne DNA to monitor crop pathogens and pests
Summary: Crop pests and diseases increasingly challenge the global food system. To prepare for and detect outbreaks, surveillance plays an important role. Traditional monitoring methods are often organism-specific, making large-scale monitoring of crop pathogens and pests impractical. We here invest...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-07-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225011733 |
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| author | Amanda Mikko Jose Antonio Villegas Daniel Svensson Edvin Karlsson Per-Anders Esseen Benedicte Riber Albrectsen Ola Lundin Mats Forsman Anna Berlin Per Stenberg |
| author_facet | Amanda Mikko Jose Antonio Villegas Daniel Svensson Edvin Karlsson Per-Anders Esseen Benedicte Riber Albrectsen Ola Lundin Mats Forsman Anna Berlin Per Stenberg |
| author_sort | Amanda Mikko |
| collection | DOAJ |
| description | Summary: Crop pests and diseases increasingly challenge the global food system. To prepare for and detect outbreaks, surveillance plays an important role. Traditional monitoring methods are often organism-specific, making large-scale monitoring of crop pathogens and pests impractical. We here investigate the potential for using shotgun sequencing of airborne eDNA for large-scale surveillance of crop pathogens and pests. We show that it is possible to detect DNA from all types of organisms in air, and that DNA can be classified down to species level. However, the accuracy of the identification is highly dependent on the quality of reference genomes of both the pathogens or pests, and their close relatives present in the region. Finally, we find that observed degree of crop damages correlate with amount of DNA from crop pathogens and pests in air, showing the promise of this approach for surveillance of all types of crop pathogens and pests. |
| format | Article |
| id | doaj-art-7caafb3cdcc549c1b241e9423ef60ad0 |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-7caafb3cdcc549c1b241e9423ef60ad02025-08-20T03:32:50ZengElsevieriScience2589-00422025-07-0128711291210.1016/j.isci.2025.112912Sequencing airborne DNA to monitor crop pathogens and pestsAmanda Mikko0Jose Antonio Villegas1Daniel Svensson2Edvin Karlsson3Per-Anders Esseen4Benedicte Riber Albrectsen5Ola Lundin6Mats Forsman7Anna Berlin8Per Stenberg9Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, Umeå, SwedenDepartment of Ecology and Environmental Sciences, Umeå University, Umeå, SwedenDepartment of Ecology and Environmental Sciences, Umeå University, Umeå, SwedenDepartment of Ecology and Environmental Sciences, Umeå University, Umeå, Sweden; CBRN Defence and Security, Swedish Defence Research Agency (FOI), Umeå, SwedenDepartment of Ecology and Environmental Sciences, Umeå University, Umeå, SwedenUmeå Plant Science Centre, Department of Plant Physiology, Umeå University, Umeå, SwedenDepartment of Ecology, Swedish University of Agricultural Sciences, Uppsala, SwedenCBRN Defence and Security, Swedish Defence Research Agency (FOI), Umeå, SwedenDepartment of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, Uppsala, SwedenDepartment of Ecology and Environmental Sciences, Umeå University, Umeå, Sweden; CBRN Defence and Security, Swedish Defence Research Agency (FOI), Umeå, Sweden; Corresponding authorSummary: Crop pests and diseases increasingly challenge the global food system. To prepare for and detect outbreaks, surveillance plays an important role. Traditional monitoring methods are often organism-specific, making large-scale monitoring of crop pathogens and pests impractical. We here investigate the potential for using shotgun sequencing of airborne eDNA for large-scale surveillance of crop pathogens and pests. We show that it is possible to detect DNA from all types of organisms in air, and that DNA can be classified down to species level. However, the accuracy of the identification is highly dependent on the quality of reference genomes of both the pathogens or pests, and their close relatives present in the region. Finally, we find that observed degree of crop damages correlate with amount of DNA from crop pathogens and pests in air, showing the promise of this approach for surveillance of all types of crop pathogens and pests.http://www.sciencedirect.com/science/article/pii/S2589004225011733Environmental monitoringEnvironmental biotechnologyOmicsGenomicsAgricultural science |
| spellingShingle | Amanda Mikko Jose Antonio Villegas Daniel Svensson Edvin Karlsson Per-Anders Esseen Benedicte Riber Albrectsen Ola Lundin Mats Forsman Anna Berlin Per Stenberg Sequencing airborne DNA to monitor crop pathogens and pests iScience Environmental monitoring Environmental biotechnology Omics Genomics Agricultural science |
| title | Sequencing airborne DNA to monitor crop pathogens and pests |
| title_full | Sequencing airborne DNA to monitor crop pathogens and pests |
| title_fullStr | Sequencing airborne DNA to monitor crop pathogens and pests |
| title_full_unstemmed | Sequencing airborne DNA to monitor crop pathogens and pests |
| title_short | Sequencing airborne DNA to monitor crop pathogens and pests |
| title_sort | sequencing airborne dna to monitor crop pathogens and pests |
| topic | Environmental monitoring Environmental biotechnology Omics Genomics Agricultural science |
| url | http://www.sciencedirect.com/science/article/pii/S2589004225011733 |
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