ESCRT may function as a tumor biomarker, transitioning from pan-cancer analysis to validation within breast cancer
BackgroundStudies have shown that ESCRT genes affect cell aging, immune environment, and tumors. BRCA was used to explore its effects on tumor prognosis and therapy.MethodsThe data sets of Cancer Genome Atlas (TCGA), Genome-Tissue Expression Plan (GTEX), Human Protein Mapping (HPA), Gene Expression...
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Frontiers Media S.A.
2025-03-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1531940/full |
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| author | Xiao-rui Chen Xiao-rui Chen Xue-ying Tan Zong-liang Zhang Jiang-shui Yuan Wei-qing Song Wei-qing Song |
| author_facet | Xiao-rui Chen Xiao-rui Chen Xue-ying Tan Zong-liang Zhang Jiang-shui Yuan Wei-qing Song Wei-qing Song |
| author_sort | Xiao-rui Chen |
| collection | DOAJ |
| description | BackgroundStudies have shown that ESCRT genes affect cell aging, immune environment, and tumors. BRCA was used to explore its effects on tumor prognosis and therapy.MethodsThe data sets of Cancer Genome Atlas (TCGA), Genome-Tissue Expression Plan (GTEX), Human Protein Mapping (HPA), Gene Expression Omnibus (GEO), Clinical Proteomic Tumor Analysis Consortium (CPTAC), R software package, and bioinformatics methods were used to mine the potential carcinogenic effects of ESCRT, including the expression level, prognostic value, and immune value of ESCRT in various types of tumor tissues, and the potential function of ESCRT family genes was further verified in breast cancer.ResultsESCRT showed significant differential expression in various cancers, such as bladder urothelial carcinoma and liver, cervical, renal, esophageal, head, and neck cancers (P <0.05). Abnormal ESCRT expression is associated with poor prognosis in various cancers, such as adrenocortical carcinoma, bladder urothelial carcinoma, breast cancer, and cervical cancer (P <0.05). The expression level of ESCRT was significantly associated with immune cell infiltration and the modulation of the stromal/immune score (all P <0.05). Enrichment analysis showed that ESCRT is associated with immune-related functions and transport signaling pathways in various tumor cells. Moreover, ESCRT serves as an early diagnostic marker for several tumors and is significantly associated with prognosis. This confirms that ESCRT is associated with most immune-infiltrating cells in pan-carcinomas. Taken together, these studies highlight the importance of the ESCRT family gene VPS37D in breast cancer initiation, progression, and immune response.ConclusionThis study highlights ESCRT’s potential in tumor detection via pan-cancer analysis, showing expression variations between tumor and normal tissues, its role in cancer progression through the immune microenvironment, and its specificity and sensitivity in detection. The VPS37D gene, with significant variation in breast cancer, predicts patient prognosis and is related to the tumor microenvironment, suggesting that ESCRT is a novel biomarker for early diagnosis and prognosis assessment. |
| format | Article |
| id | doaj-art-7ca96d8d614849779b903e63f11dd4ba |
| institution | OA Journals |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj-art-7ca96d8d614849779b903e63f11dd4ba2025-08-20T02:10:53ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-03-011610.3389/fimmu.2025.15319401531940ESCRT may function as a tumor biomarker, transitioning from pan-cancer analysis to validation within breast cancerXiao-rui Chen0Xiao-rui Chen1Xue-ying Tan2Zong-liang Zhang3Jiang-shui Yuan4Wei-qing Song5Wei-qing Song6Qingdao Medical College, Qingdao University, Qingdao, ChinaClinical Laboratory Department, Qingdao Municipal Hospital, Qingdao, ChinaClinical Laboratory Department, Qingdao Municipal Hospital, Qingdao, ChinaDepartment of Urology, Affiliated Hospital of Qingdao University, Qingdao, ChinaClinical Laboratory Department, Qingdao Municipal Hospital, Qingdao, ChinaQingdao Medical College, Qingdao University, Qingdao, ChinaClinical Laboratory Department, Qingdao Municipal Hospital, Qingdao, ChinaBackgroundStudies have shown that ESCRT genes affect cell aging, immune environment, and tumors. BRCA was used to explore its effects on tumor prognosis and therapy.MethodsThe data sets of Cancer Genome Atlas (TCGA), Genome-Tissue Expression Plan (GTEX), Human Protein Mapping (HPA), Gene Expression Omnibus (GEO), Clinical Proteomic Tumor Analysis Consortium (CPTAC), R software package, and bioinformatics methods were used to mine the potential carcinogenic effects of ESCRT, including the expression level, prognostic value, and immune value of ESCRT in various types of tumor tissues, and the potential function of ESCRT family genes was further verified in breast cancer.ResultsESCRT showed significant differential expression in various cancers, such as bladder urothelial carcinoma and liver, cervical, renal, esophageal, head, and neck cancers (P <0.05). Abnormal ESCRT expression is associated with poor prognosis in various cancers, such as adrenocortical carcinoma, bladder urothelial carcinoma, breast cancer, and cervical cancer (P <0.05). The expression level of ESCRT was significantly associated with immune cell infiltration and the modulation of the stromal/immune score (all P <0.05). Enrichment analysis showed that ESCRT is associated with immune-related functions and transport signaling pathways in various tumor cells. Moreover, ESCRT serves as an early diagnostic marker for several tumors and is significantly associated with prognosis. This confirms that ESCRT is associated with most immune-infiltrating cells in pan-carcinomas. Taken together, these studies highlight the importance of the ESCRT family gene VPS37D in breast cancer initiation, progression, and immune response.ConclusionThis study highlights ESCRT’s potential in tumor detection via pan-cancer analysis, showing expression variations between tumor and normal tissues, its role in cancer progression through the immune microenvironment, and its specificity and sensitivity in detection. The VPS37D gene, with significant variation in breast cancer, predicts patient prognosis and is related to the tumor microenvironment, suggesting that ESCRT is a novel biomarker for early diagnosis and prognosis assessment.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1531940/fullpan-cancerESCRTbiomarkerimmune cell infiltrationVPS37D |
| spellingShingle | Xiao-rui Chen Xiao-rui Chen Xue-ying Tan Zong-liang Zhang Jiang-shui Yuan Wei-qing Song Wei-qing Song ESCRT may function as a tumor biomarker, transitioning from pan-cancer analysis to validation within breast cancer Frontiers in Immunology pan-cancer ESCRT biomarker immune cell infiltration VPS37D |
| title | ESCRT may function as a tumor biomarker, transitioning from pan-cancer analysis to validation within breast cancer |
| title_full | ESCRT may function as a tumor biomarker, transitioning from pan-cancer analysis to validation within breast cancer |
| title_fullStr | ESCRT may function as a tumor biomarker, transitioning from pan-cancer analysis to validation within breast cancer |
| title_full_unstemmed | ESCRT may function as a tumor biomarker, transitioning from pan-cancer analysis to validation within breast cancer |
| title_short | ESCRT may function as a tumor biomarker, transitioning from pan-cancer analysis to validation within breast cancer |
| title_sort | escrt may function as a tumor biomarker transitioning from pan cancer analysis to validation within breast cancer |
| topic | pan-cancer ESCRT biomarker immune cell infiltration VPS37D |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1531940/full |
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