Increased Responsiveness of Human Coronary Artery Endothelial Cells in Inflammation and Coagulation
The effects of anti-inflammatory plant extracts, such as black tea extract (BTE) and resveratrol (RSV) could modulate cell activation leading to atherosclerosis, however there is little comparative information about how different endothelial cell types are affected by these compounds. In order to co...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2009-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2009/146872 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849409602519564288 |
|---|---|
| author | Katja Lakota Katjusa Mrak-Poljsak Blaz Rozman Snezna Sodin-Semrl |
| author_facet | Katja Lakota Katjusa Mrak-Poljsak Blaz Rozman Snezna Sodin-Semrl |
| author_sort | Katja Lakota |
| collection | DOAJ |
| description | The effects of anti-inflammatory plant extracts, such as black tea extract (BTE) and resveratrol (RSV) could modulate cell activation leading to atherosclerosis, however there is little comparative information about how different endothelial cell types are affected by these compounds. In order to compare human endothelial cells derived from different origins (umbilical vein or HUVEC, coronary artery or HCAEC, microvascular or HMVEC) and their interleukin-1β (IL-1β) responsiveness, IL-6 ELISA, RT-PCR, tissue factor assay, and prostacyclin responses using 6-keto PGF1α ELISA were determined. The IL-1β-induced IL-6 levels were dose-dependent with highest responses seen in HCAEC. Significant inhibition of IL-1β responses was achieved with BTE and RSV, with the largest decrease of IL-6 and TF seen in HCAEC. Prostacyclin levels were highest in HUVEC and were inhibited by RSV in all cell types. The differences between the endothelial cell types could account for greater susceptibility of coronary arteries to inflammation and atherogenesis. |
| format | Article |
| id | doaj-art-7ca6a32bc64d4da0866603d34eb7c3ed |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-7ca6a32bc64d4da0866603d34eb7c3ed2025-08-20T03:35:27ZengWileyMediators of Inflammation0962-93511466-18612009-01-01200910.1155/2009/146872146872Increased Responsiveness of Human Coronary Artery Endothelial Cells in Inflammation and CoagulationKatja Lakota0Katjusa Mrak-Poljsak1Blaz Rozman2Snezna Sodin-Semrl3Department of Rheumatology, University Medical Centre, Vodnikova 62, 1000 Ljubljana, SloveniaDepartment of Rheumatology, University Medical Centre, Vodnikova 62, 1000 Ljubljana, SloveniaDepartment of Rheumatology, University Medical Centre, Vodnikova 62, 1000 Ljubljana, SloveniaDepartment of Rheumatology, University Medical Centre, Vodnikova 62, 1000 Ljubljana, SloveniaThe effects of anti-inflammatory plant extracts, such as black tea extract (BTE) and resveratrol (RSV) could modulate cell activation leading to atherosclerosis, however there is little comparative information about how different endothelial cell types are affected by these compounds. In order to compare human endothelial cells derived from different origins (umbilical vein or HUVEC, coronary artery or HCAEC, microvascular or HMVEC) and their interleukin-1β (IL-1β) responsiveness, IL-6 ELISA, RT-PCR, tissue factor assay, and prostacyclin responses using 6-keto PGF1α ELISA were determined. The IL-1β-induced IL-6 levels were dose-dependent with highest responses seen in HCAEC. Significant inhibition of IL-1β responses was achieved with BTE and RSV, with the largest decrease of IL-6 and TF seen in HCAEC. Prostacyclin levels were highest in HUVEC and were inhibited by RSV in all cell types. The differences between the endothelial cell types could account for greater susceptibility of coronary arteries to inflammation and atherogenesis.http://dx.doi.org/10.1155/2009/146872 |
| spellingShingle | Katja Lakota Katjusa Mrak-Poljsak Blaz Rozman Snezna Sodin-Semrl Increased Responsiveness of Human Coronary Artery Endothelial Cells in Inflammation and Coagulation Mediators of Inflammation |
| title | Increased Responsiveness of Human Coronary Artery Endothelial Cells in Inflammation and Coagulation |
| title_full | Increased Responsiveness of Human Coronary Artery Endothelial Cells in Inflammation and Coagulation |
| title_fullStr | Increased Responsiveness of Human Coronary Artery Endothelial Cells in Inflammation and Coagulation |
| title_full_unstemmed | Increased Responsiveness of Human Coronary Artery Endothelial Cells in Inflammation and Coagulation |
| title_short | Increased Responsiveness of Human Coronary Artery Endothelial Cells in Inflammation and Coagulation |
| title_sort | increased responsiveness of human coronary artery endothelial cells in inflammation and coagulation |
| url | http://dx.doi.org/10.1155/2009/146872 |
| work_keys_str_mv | AT katjalakota increasedresponsivenessofhumancoronaryarteryendothelialcellsininflammationandcoagulation AT katjusamrakpoljsak increasedresponsivenessofhumancoronaryarteryendothelialcellsininflammationandcoagulation AT blazrozman increasedresponsivenessofhumancoronaryarteryendothelialcellsininflammationandcoagulation AT sneznasodinsemrl increasedresponsivenessofhumancoronaryarteryendothelialcellsininflammationandcoagulation |