Cloning and expression of α-acetolactate decarboxylase gene from Enterobacter aerogenes in Escherichia coli
The ALDC gene was cloned by PCR using Enterobactr aerogenes chromosome DNA as template and got 0.78 kb DNA fragment. The fragment was inserted directionally into a prokaryotic expression vector pQE-30 with high efficiency. The ALDC gene was overexpressed in E. coli M15, and the production of ALDC wa...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2000-05-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2000.03.0291 |
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| Summary: | The ALDC gene was cloned by PCR using Enterobactr aerogenes chromosome DNA as template and got 0.78 kb DNA fragment. The fragment was inserted directionally into a prokaryotic expression vector pQE-30 with high efficiency. The ALDC gene was overexpressed in E. coli M15, and the production of ALDC was over 180 U/mL, 100 times more than the parented strain. The recombinant strain was still stable after 67 generations at 37℃ without antibiotic selection. |
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| ISSN: | 1008-9209 2097-5155 |