Genome-wide identification and characterization of the Lateral Organ Boundaries Domain (LBD) gene family in nine Rosaceae species and expression pattern in Prunus mume

Transcription factors (TFs) encoded by the lateral organ boundaries domain (LBD) gene family are known to control many plant-specific developmental processes. However, the comparative analysis of the LBD gene family in Rosaceae species and its expression pattern in mei remains unclear. Here, we iden...

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Main Authors: Weichao Liu, Xiaoyu Guo, Tangchun Zheng, Xue Li, Sagheer Ahmad, Jia Wang, Qixiang Zhang, Tangren Cheng
Format: Article
Language:English
Published: Maximum Academic Press 2024-01-01
Series:Ornamental Plant Research
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Online Access:https://www.maxapress.com/article/doi/10.48130/opr-0024-0005
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author Weichao Liu
Xiaoyu Guo
Tangchun Zheng
Xue Li
Sagheer Ahmad
Jia Wang
Qixiang Zhang
Tangren Cheng
author_facet Weichao Liu
Xiaoyu Guo
Tangchun Zheng
Xue Li
Sagheer Ahmad
Jia Wang
Qixiang Zhang
Tangren Cheng
author_sort Weichao Liu
collection DOAJ
description Transcription factors (TFs) encoded by the lateral organ boundaries domain (LBD) gene family are known to control many plant-specific developmental processes. However, the comparative analysis of the LBD gene family in Rosaceae species and its expression pattern in mei remains unclear. Here, we identified a total of 406 LBDs in nine Rosaceae species, including 39 in black raspberry (Rubus occidentalis), 34 in strawberry (Fragaria vesca), 39 in Chinese rose (Rosa chinensis), 42 in peach (Prunus persica), 41 in apricot (Prunus armeniaca), 41 in mei (Prunus mume var. tortuosa), 60 in pear (Pyrus communis), 41 in hawthorn (Crataegus pinnatifida) and 69 in apple (Malus domestica), respectively. The LBDs of nine Rosaceae species were classified into seven major subclasses. The chromosome localization, collinearity analysis, and gene duplication relationship revealed that segment duplication was the main driving force for the amplification of LBDs in the Rosoideae and Amygdaloideae. Ka/Ks analysis suggested most of the LBD gene pairs might be under purification selection. GO and cis-acting elements analysis showed that LBDs may play important roles in many biological processes and could respond to hormones and stresses. RNA-seq data showed that PmLBD17/19/41 genes contained both low-temperature and MeJA response elements and played a significant variation across different geographic locations and periods. PmLBD30, the ortholog of EgLBD29, exhibited an up-regulation followed by a decrease, which is hypothesized to possibly play a role in the formation of a weeping trait in mei. Our studies offer important data about the development of the LBD family in Rosaceae and the subsequent validation of LBDs' functional genes in P. mume.
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spelling doaj-art-7c90970aa7504a0496eecf9d2bfc41f52025-08-20T02:27:14ZengMaximum Academic PressOrnamental Plant Research2769-20942024-01-014111410.48130/opr-0024-0005opr-0024-0005Genome-wide identification and characterization of the Lateral Organ Boundaries Domain (LBD) gene family in nine Rosaceae species and expression pattern in Prunus mumeWeichao Liu0Xiaoyu Guo1Tangchun Zheng2Xue Li3Sagheer Ahmad4Jia Wang5Qixiang Zhang6Tangren Cheng7Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Engineering Research Center of Landscape Environment of Ministry of Education, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Engineering Research Center of Landscape Environment of Ministry of Education, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Engineering Research Center of Landscape Environment of Ministry of Education, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Engineering Research Center of Landscape Environment of Ministry of Education, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaKey Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Engineering Research Center of Landscape Environment of Ministry of Education, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Engineering Research Center of Landscape Environment of Ministry of Education, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Engineering Research Center of Landscape Environment of Ministry of Education, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaTranscription factors (TFs) encoded by the lateral organ boundaries domain (LBD) gene family are known to control many plant-specific developmental processes. However, the comparative analysis of the LBD gene family in Rosaceae species and its expression pattern in mei remains unclear. Here, we identified a total of 406 LBDs in nine Rosaceae species, including 39 in black raspberry (Rubus occidentalis), 34 in strawberry (Fragaria vesca), 39 in Chinese rose (Rosa chinensis), 42 in peach (Prunus persica), 41 in apricot (Prunus armeniaca), 41 in mei (Prunus mume var. tortuosa), 60 in pear (Pyrus communis), 41 in hawthorn (Crataegus pinnatifida) and 69 in apple (Malus domestica), respectively. The LBDs of nine Rosaceae species were classified into seven major subclasses. The chromosome localization, collinearity analysis, and gene duplication relationship revealed that segment duplication was the main driving force for the amplification of LBDs in the Rosoideae and Amygdaloideae. Ka/Ks analysis suggested most of the LBD gene pairs might be under purification selection. GO and cis-acting elements analysis showed that LBDs may play important roles in many biological processes and could respond to hormones and stresses. RNA-seq data showed that PmLBD17/19/41 genes contained both low-temperature and MeJA response elements and played a significant variation across different geographic locations and periods. PmLBD30, the ortholog of EgLBD29, exhibited an up-regulation followed by a decrease, which is hypothesized to possibly play a role in the formation of a weeping trait in mei. Our studies offer important data about the development of the LBD family in Rosaceae and the subsequent validation of LBDs' functional genes in P. mume.https://www.maxapress.com/article/doi/10.48130/opr-0024-0005rosaceaelbd gene familyprunus mumephylogenetic analysiscollinearity analysisexpression pattern
spellingShingle Weichao Liu
Xiaoyu Guo
Tangchun Zheng
Xue Li
Sagheer Ahmad
Jia Wang
Qixiang Zhang
Tangren Cheng
Genome-wide identification and characterization of the Lateral Organ Boundaries Domain (LBD) gene family in nine Rosaceae species and expression pattern in Prunus mume
Ornamental Plant Research
rosaceae
lbd gene family
prunus mume
phylogenetic analysis
collinearity analysis
expression pattern
title Genome-wide identification and characterization of the Lateral Organ Boundaries Domain (LBD) gene family in nine Rosaceae species and expression pattern in Prunus mume
title_full Genome-wide identification and characterization of the Lateral Organ Boundaries Domain (LBD) gene family in nine Rosaceae species and expression pattern in Prunus mume
title_fullStr Genome-wide identification and characterization of the Lateral Organ Boundaries Domain (LBD) gene family in nine Rosaceae species and expression pattern in Prunus mume
title_full_unstemmed Genome-wide identification and characterization of the Lateral Organ Boundaries Domain (LBD) gene family in nine Rosaceae species and expression pattern in Prunus mume
title_short Genome-wide identification and characterization of the Lateral Organ Boundaries Domain (LBD) gene family in nine Rosaceae species and expression pattern in Prunus mume
title_sort genome wide identification and characterization of the lateral organ boundaries domain lbd gene family in nine rosaceae species and expression pattern in prunus mume
topic rosaceae
lbd gene family
prunus mume
phylogenetic analysis
collinearity analysis
expression pattern
url https://www.maxapress.com/article/doi/10.48130/opr-0024-0005
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