Deletion of the PHO91 Gene Leads to Impaired Ability to Consume Methanol in Cells of the Methylotrophic Yeast <i>Ogataea Parapolymorpha</i>
The PHO91 and PHO87 are parts of yeast’s phosphate metabolism system. We investigated the influence of PHO91 deletion on the methanol utilization as a sole carbon source in <i>Ogataea parapolymorpha</i> through comparison of wet biomass, protein content, methanol oxidase (MOX) activity a...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-05-01
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| Series: | Proceedings |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2504-3900/105/1/83 |
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| Summary: | The PHO91 and PHO87 are parts of yeast’s phosphate metabolism system. We investigated the influence of PHO91 deletion on the methanol utilization as a sole carbon source in <i>Ogataea parapolymorpha</i> through comparison of wet biomass, protein content, methanol oxidase (MOX) activity and acid-soluble and acid-insoluble inorganic polyphosphate (polyP) content, while growing on methanol and without any carbon source other than trace amounts from the yeast extract. The <i>Δpho91</i> strain has a lack of wet biomass, protein content and MOX activity while grown on methanol (as a <i>ΔMOX</i> strain) and has the same numbers as a WT strain in media without methanol. We found two ways of recovering methanol utilizing capabilities for <i>Δpho91</i> mutant—either adding MOX on plasmid under constitutive promoter control, or adding a <i>Δpho87</i> mutation. Both <i>Δpho91</i> + MOX and <i>Δpho91Δpho87</i> strains showed all the parameters as a WT strain on methanol as a sole carbon source and without methanol addition. Further, we compare the polyP content of the strains in methanol media, media without a sole carbon source and media with glucose. All of the WT, <i>Δpho91</i> + MOX and <i>Δpho91Δpho87</i> strains, capable of utilizing methanol, have the P<sub>i</sub>, acid-soluble and acid-insoluble polyP at the same levels with some variations. The <i>ΔMOX</i> and <i>Δpho91</i> strains, despite both not being able to utilize methanol, showed very different levels of P<sub>i</sub> and polyP’s, which can be interpreted as different mechanisms of the inner cell, leading to a loss of capability utilizing methanol in both of these strains. |
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| ISSN: | 2504-3900 |