Changes in the intestinal microbiota induced by the postnatal environment and their association with hypertension
It has been established that cross-fostering impacts the development of hypertension in spontaneously hypertensive rats (SHR). However, the ability of the cross-fostering protocol to shape gut microbiota profile in SHR and impact hypertension is not known. In this sense, the current study explored t...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-02-01
|
| Series: | Pharmacological Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1043661825000465 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1825199427682304000 |
|---|---|
| author | Patrizia Dardi Camille Perella Coutinho Sarah de Oliveira Simone Aparecida Teixeira Renaide Rodrigues Ferreira Gacek Eduardo Purgatto Marco Aurélio Ramirez Vinolo Marcelo Nicolás Muscará Luciana Venturini Rossoni |
| author_facet | Patrizia Dardi Camille Perella Coutinho Sarah de Oliveira Simone Aparecida Teixeira Renaide Rodrigues Ferreira Gacek Eduardo Purgatto Marco Aurélio Ramirez Vinolo Marcelo Nicolás Muscará Luciana Venturini Rossoni |
| author_sort | Patrizia Dardi |
| collection | DOAJ |
| description | It has been established that cross-fostering impacts the development of hypertension in spontaneously hypertensive rats (SHR). However, the ability of the cross-fostering protocol to shape gut microbiota profile in SHR and impact hypertension is not known. In this sense, the current study explored the influence of normotensive and hypertensive postnatal environments on the intestinal microbiota structure, composition, and functional capacity of SHR and Wistar rats. Our findings revealed significant differences in the microbiota's composition and its metabolic activity in young non-fostered SHR (SS) vs. Wistar (WW) rats, even before hypertension onset, characterized by a reduction of the “low-abundance” bacterial genera, a diminished availability of fecal butyrate and elevated hydrogen sulfide (H2S) production by the SS gut microbiota. Despite influencing the microbiota of both strains, cross-fostering did not fully replicate the microbiota composition of the naturally reared groups in the SHR nursed by Wistar mothers (SW), or in the Wistar rats breastfed by SHR mothers (WS). The SW group had fewer significant genera identified at the Partial Least Squares Discriminant Analysis (PLS-DA), despite resembling the genera profile identified in the normotensive group. While sharing bacterial genera with both SS and WW groups, the WS group is distinguished by its unique microbial composition, particularly by a greater diversity of the ‘low-abundance’ bacterial genera. Moreover, decreased systolic blood pressure was observed in the SW group compared to the SS group in adulthood. Thus, we could establish a link between microbiota composition and hypertension development, associating it with the loss of the ''low-abundance'' bacterial taxa. Our data suggest that the postnatal environment is pivotal to promoting gut microbiota compositional changes and contributes to hypertension development. |
| format | Article |
| id | doaj-art-7c7674b9f97d4dac9f828d4e2a2598d4 |
| institution | Kabale University |
| issn | 1096-1186 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Pharmacological Research |
| spelling | doaj-art-7c7674b9f97d4dac9f828d4e2a2598d42025-02-08T04:59:53ZengElsevierPharmacological Research1096-11862025-02-01212107621Changes in the intestinal microbiota induced by the postnatal environment and their association with hypertensionPatrizia Dardi0Camille Perella Coutinho1Sarah de Oliveira2Simone Aparecida Teixeira3Renaide Rodrigues Ferreira Gacek4Eduardo Purgatto5Marco Aurélio Ramirez Vinolo6Marcelo Nicolás Muscará7Luciana Venturini Rossoni8Laboratory of Vascular Physiology, Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, BrazilDepartment of Food and Experimental Nutrition /FoRC - Food Research Center, University of Sao Paulo, Sao Paulo, BrazilLaboratory of Immunoinflammation, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP), Campinas, BrazilDepartment of Pharmacology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, BrazilAnimal production facility ‘Zuleica Bruno Fortes’, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, BrazilDepartment of Food and Experimental Nutrition /FoRC - Food Research Center, University of Sao Paulo, Sao Paulo, BrazilLaboratory of Immunoinflammation, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP), Campinas, BrazilDepartment of Pharmacology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, BrazilLaboratory of Vascular Physiology, Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil; Correspondence to: Instituto de Ciências Biomédicas – ICB I, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1524 – Room 225, Cidade Universitária – Butantã, São Paulo, SP CEP: 05508-000, Brasil.It has been established that cross-fostering impacts the development of hypertension in spontaneously hypertensive rats (SHR). However, the ability of the cross-fostering protocol to shape gut microbiota profile in SHR and impact hypertension is not known. In this sense, the current study explored the influence of normotensive and hypertensive postnatal environments on the intestinal microbiota structure, composition, and functional capacity of SHR and Wistar rats. Our findings revealed significant differences in the microbiota's composition and its metabolic activity in young non-fostered SHR (SS) vs. Wistar (WW) rats, even before hypertension onset, characterized by a reduction of the “low-abundance” bacterial genera, a diminished availability of fecal butyrate and elevated hydrogen sulfide (H2S) production by the SS gut microbiota. Despite influencing the microbiota of both strains, cross-fostering did not fully replicate the microbiota composition of the naturally reared groups in the SHR nursed by Wistar mothers (SW), or in the Wistar rats breastfed by SHR mothers (WS). The SW group had fewer significant genera identified at the Partial Least Squares Discriminant Analysis (PLS-DA), despite resembling the genera profile identified in the normotensive group. While sharing bacterial genera with both SS and WW groups, the WS group is distinguished by its unique microbial composition, particularly by a greater diversity of the ‘low-abundance’ bacterial genera. Moreover, decreased systolic blood pressure was observed in the SW group compared to the SS group in adulthood. Thus, we could establish a link between microbiota composition and hypertension development, associating it with the loss of the ''low-abundance'' bacterial taxa. Our data suggest that the postnatal environment is pivotal to promoting gut microbiota compositional changes and contributes to hypertension development.http://www.sciencedirect.com/science/article/pii/S1043661825000465Gut microbiotaSHRCross-FosteringSCFAsHydrogen sulfide |
| spellingShingle | Patrizia Dardi Camille Perella Coutinho Sarah de Oliveira Simone Aparecida Teixeira Renaide Rodrigues Ferreira Gacek Eduardo Purgatto Marco Aurélio Ramirez Vinolo Marcelo Nicolás Muscará Luciana Venturini Rossoni Changes in the intestinal microbiota induced by the postnatal environment and their association with hypertension Pharmacological Research Gut microbiota SHR Cross-Fostering SCFAs Hydrogen sulfide |
| title | Changes in the intestinal microbiota induced by the postnatal environment and their association with hypertension |
| title_full | Changes in the intestinal microbiota induced by the postnatal environment and their association with hypertension |
| title_fullStr | Changes in the intestinal microbiota induced by the postnatal environment and their association with hypertension |
| title_full_unstemmed | Changes in the intestinal microbiota induced by the postnatal environment and their association with hypertension |
| title_short | Changes in the intestinal microbiota induced by the postnatal environment and their association with hypertension |
| title_sort | changes in the intestinal microbiota induced by the postnatal environment and their association with hypertension |
| topic | Gut microbiota SHR Cross-Fostering SCFAs Hydrogen sulfide |
| url | http://www.sciencedirect.com/science/article/pii/S1043661825000465 |
| work_keys_str_mv | AT patriziadardi changesintheintestinalmicrobiotainducedbythepostnatalenvironmentandtheirassociationwithhypertension AT camilleperellacoutinho changesintheintestinalmicrobiotainducedbythepostnatalenvironmentandtheirassociationwithhypertension AT sarahdeoliveira changesintheintestinalmicrobiotainducedbythepostnatalenvironmentandtheirassociationwithhypertension AT simoneaparecidateixeira changesintheintestinalmicrobiotainducedbythepostnatalenvironmentandtheirassociationwithhypertension AT renaiderodriguesferreiragacek changesintheintestinalmicrobiotainducedbythepostnatalenvironmentandtheirassociationwithhypertension AT eduardopurgatto changesintheintestinalmicrobiotainducedbythepostnatalenvironmentandtheirassociationwithhypertension AT marcoaurelioramirezvinolo changesintheintestinalmicrobiotainducedbythepostnatalenvironmentandtheirassociationwithhypertension AT marcelonicolasmuscara changesintheintestinalmicrobiotainducedbythepostnatalenvironmentandtheirassociationwithhypertension AT lucianaventurinirossoni changesintheintestinalmicrobiotainducedbythepostnatalenvironmentandtheirassociationwithhypertension |