Drop-on-demand printing of amine-responsive fluorescence-ratiometric sensor array for online monitoring meat freshness
Aiming to enable online freshness-monitoring of meat within modified-atmosphere package, we developed a ratiometric array that was fluorescently responsive to volatile organic compounds-ammonia (NH3) released by protein decaying. The array was consisted of two 3 mm × 6 mm rectangles precisely and un...
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Format: | Article |
Language: | English |
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Elsevier
2025-01-01
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Series: | Food Chemistry: X |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157524009878 |
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author | Zhijian Wang Xudong Shi Jingze Guo Lin Wang Meilin Cao Shiyao Wang Yisheng Chen |
author_facet | Zhijian Wang Xudong Shi Jingze Guo Lin Wang Meilin Cao Shiyao Wang Yisheng Chen |
author_sort | Zhijian Wang |
collection | DOAJ |
description | Aiming to enable online freshness-monitoring of meat within modified-atmosphere package, we developed a ratiometric array that was fluorescently responsive to volatile organic compounds-ammonia (NH3) released by protein decaying. The array was consisted of two 3 mm × 6 mm rectangles precisely and uniformly printed with fluorescein isothiocyanate (FITC) as indicator and rhodamine B (RhB) as internal reference on the filter-paper, respectively. The fluorescence intensity of the array area was calibrated according to Green/Red ratio of the digitalized pixels extracted from images facilitated by a smartphone. The fluorescence-ratiometric sensor array displayed remarkable detection performances, including high sensitivity (LOD = 1.1 ppm), stability (91 % responding attenuation over 10 d of storage) and reproducibility (RSD < 10 %), which was further validated with real pork and shrimp samples. Subsequently, the fluorescent signals of the dual-rectangle array showed high correlation to the total volatile base nitrogen value that was officially used for indexing the meat freshness status. |
format | Article |
id | doaj-art-7c71063184c341ca88c12e5878604cd1 |
institution | Kabale University |
issn | 2590-1575 |
language | English |
publishDate | 2025-01-01 |
publisher | Elsevier |
record_format | Article |
series | Food Chemistry: X |
spelling | doaj-art-7c71063184c341ca88c12e5878604cd12025-02-12T05:31:57ZengElsevierFood Chemistry: X2590-15752025-01-0125102099Drop-on-demand printing of amine-responsive fluorescence-ratiometric sensor array for online monitoring meat freshnessZhijian Wang0Xudong Shi1Jingze Guo2Lin Wang3Meilin Cao4Shiyao Wang5Yisheng Chen6College of Food Science and Engineering, Shanxi Agricultural University, Taiyuan, Shanxi, ChinaCollege of Food Science and Engineering, Shanxi Agricultural University, Taiyuan, Shanxi, ChinaCollege of Food Science and Engineering, Shanxi Agricultural University, Taiyuan, Shanxi, ChinaCollege of Food Science and Engineering, Shanxi Agricultural University, Taiyuan, Shanxi, ChinaCollege of Food Science and Engineering, Shanxi Agricultural University, Taiyuan, Shanxi, ChinaCollege of Food Science and Engineering, Shanxi Agricultural University, Taiyuan, Shanxi, ChinaCorresponding author.; College of Food Science and Engineering, Shanxi Agricultural University, Taiyuan, Shanxi, ChinaAiming to enable online freshness-monitoring of meat within modified-atmosphere package, we developed a ratiometric array that was fluorescently responsive to volatile organic compounds-ammonia (NH3) released by protein decaying. The array was consisted of two 3 mm × 6 mm rectangles precisely and uniformly printed with fluorescein isothiocyanate (FITC) as indicator and rhodamine B (RhB) as internal reference on the filter-paper, respectively. The fluorescence intensity of the array area was calibrated according to Green/Red ratio of the digitalized pixels extracted from images facilitated by a smartphone. The fluorescence-ratiometric sensor array displayed remarkable detection performances, including high sensitivity (LOD = 1.1 ppm), stability (91 % responding attenuation over 10 d of storage) and reproducibility (RSD < 10 %), which was further validated with real pork and shrimp samples. Subsequently, the fluorescent signals of the dual-rectangle array showed high correlation to the total volatile base nitrogen value that was officially used for indexing the meat freshness status.http://www.sciencedirect.com/science/article/pii/S2590157524009878Fluorescence-ratiometric sensor arraySmart printingMeat freshness |
spellingShingle | Zhijian Wang Xudong Shi Jingze Guo Lin Wang Meilin Cao Shiyao Wang Yisheng Chen Drop-on-demand printing of amine-responsive fluorescence-ratiometric sensor array for online monitoring meat freshness Food Chemistry: X Fluorescence-ratiometric sensor array Smart printing Meat freshness |
title | Drop-on-demand printing of amine-responsive fluorescence-ratiometric sensor array for online monitoring meat freshness |
title_full | Drop-on-demand printing of amine-responsive fluorescence-ratiometric sensor array for online monitoring meat freshness |
title_fullStr | Drop-on-demand printing of amine-responsive fluorescence-ratiometric sensor array for online monitoring meat freshness |
title_full_unstemmed | Drop-on-demand printing of amine-responsive fluorescence-ratiometric sensor array for online monitoring meat freshness |
title_short | Drop-on-demand printing of amine-responsive fluorescence-ratiometric sensor array for online monitoring meat freshness |
title_sort | drop on demand printing of amine responsive fluorescence ratiometric sensor array for online monitoring meat freshness |
topic | Fluorescence-ratiometric sensor array Smart printing Meat freshness |
url | http://www.sciencedirect.com/science/article/pii/S2590157524009878 |
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