Digital sequencing is improved by using structured unique molecular identifiers

Digital sequencing is improved by using structured unique molecular identifiers

Abstract Digital sequencing uses unique molecular identifiers (UMIs) to correct for polymerase induced errors and amplification biases. Here, we design 19 different structured UMIs to minimize the capacity of primers to form non-specific PCR products during library construction using SiMSen-Seq, a P...

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Bibliographic Details
Main Authors: Peter Micallef, Manuel Luna Santamaría, Mandy Escobar, Daniel Andersson, Tobias Österlund, Pia Mouhanna, Stefan Filges, Gustav Johansson, Henrik Fagman, Christoffer Vannas, Anders Ståhlberg
Format: Article
Language:English
Published: BMC 2025-02-01
Series:Genome Biology
Subjects:
Digital sequencing
Error-free sequencing
Molecular barcode
Sequencing
Unique molecular identifier
Online Access:https://doi.org/10.1186/s13059-025-03504-x
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