MicroRNA-200b suppresses the invasion and migration of hepatocellular carcinoma by downregulating RhoA and circRNA_000839

MicroRNAs could mediate the targeted coding gene and the targeted non-coding RNA to form endogenous competition, which have an important regulatory role in tumorigenesis of many types of cancer, including hepatocellular carcinoma. The goal of this study was to characterize the role of miR-200b in th...

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Main Authors: Ben-gang Wang, Jun-shuai Li, Yong-feng Liu, Qian Xu
Format: Article
Language:English
Published: SAGE Publishing 2017-07-01
Series:Tumor Biology
Online Access:https://doi.org/10.1177/1010428317719577
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author Ben-gang Wang
Jun-shuai Li
Yong-feng Liu
Qian Xu
author_facet Ben-gang Wang
Jun-shuai Li
Yong-feng Liu
Qian Xu
author_sort Ben-gang Wang
collection DOAJ
description MicroRNAs could mediate the targeted coding gene and the targeted non-coding RNA to form endogenous competition, which have an important regulatory role in tumorigenesis of many types of cancer, including hepatocellular carcinoma. The goal of this study was to characterize the role of miR-200b in the pathogenesis of hepatocellular carcinoma. We identified miR-200b that was predicted to regulate RhoA and circ_000839. Our data establish that miR-200b is expressed at a relatively low level in hepatocellular carcinoma ( p  < 0.001). RhoA and circ_000839 are expressed at a relatively high level in hepatocellular carcinoma ( p  < 0.001, respectively). Our mechanistic data indicate that RhoA is a direct target of miR-200b ( p  < 0.001), binding of which affects the expression of invasion and migration in hepatocellular carcinoma cell lines ( p  < 0.05). And correlation analysis showed that miR-200b was inversely correlated with RhoA and circ_000839 ( p  = 0.012, p  = 0.002, respectively), while RhoA was positively correlated with circ_000839 ( p  < 0.001). Taken together, our data suggest that miR-200b could mediate RhoA gene and circ_000839 to form endogenous competition. And this is a direction for the association study of miR-200b and RhoA in the future.
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spelling doaj-art-7c6cfa0c6791469799248e933de7d8a82025-08-20T03:14:54ZengSAGE PublishingTumor Biology1423-03802017-07-013910.1177/1010428317719577MicroRNA-200b suppresses the invasion and migration of hepatocellular carcinoma by downregulating RhoA and circRNA_000839Ben-gang Wang0Jun-shuai Li1Yong-feng Liu2Qian Xu3Hepatobiliary Surgery Department of General Surgery Institute, The First Affiliated Hospital of China Medical University, Shenyang, ChinaHepatobiliary Surgery Department of General Surgery Institute, The First Affiliated Hospital of China Medical University, Shenyang, ChinaHepatobiliary Surgery Department of General Surgery Institute, The First Affiliated Hospital of China Medical University, Shenyang, ChinaTumor Etiology and Screening Department of General Surgery Institute, The First Affiliated Hospital of China Medical University, Shenyang, ChinaMicroRNAs could mediate the targeted coding gene and the targeted non-coding RNA to form endogenous competition, which have an important regulatory role in tumorigenesis of many types of cancer, including hepatocellular carcinoma. The goal of this study was to characterize the role of miR-200b in the pathogenesis of hepatocellular carcinoma. We identified miR-200b that was predicted to regulate RhoA and circ_000839. Our data establish that miR-200b is expressed at a relatively low level in hepatocellular carcinoma ( p  < 0.001). RhoA and circ_000839 are expressed at a relatively high level in hepatocellular carcinoma ( p  < 0.001, respectively). Our mechanistic data indicate that RhoA is a direct target of miR-200b ( p  < 0.001), binding of which affects the expression of invasion and migration in hepatocellular carcinoma cell lines ( p  < 0.05). And correlation analysis showed that miR-200b was inversely correlated with RhoA and circ_000839 ( p  = 0.012, p  = 0.002, respectively), while RhoA was positively correlated with circ_000839 ( p  < 0.001). Taken together, our data suggest that miR-200b could mediate RhoA gene and circ_000839 to form endogenous competition. And this is a direction for the association study of miR-200b and RhoA in the future.https://doi.org/10.1177/1010428317719577
spellingShingle Ben-gang Wang
Jun-shuai Li
Yong-feng Liu
Qian Xu
MicroRNA-200b suppresses the invasion and migration of hepatocellular carcinoma by downregulating RhoA and circRNA_000839
Tumor Biology
title MicroRNA-200b suppresses the invasion and migration of hepatocellular carcinoma by downregulating RhoA and circRNA_000839
title_full MicroRNA-200b suppresses the invasion and migration of hepatocellular carcinoma by downregulating RhoA and circRNA_000839
title_fullStr MicroRNA-200b suppresses the invasion and migration of hepatocellular carcinoma by downregulating RhoA and circRNA_000839
title_full_unstemmed MicroRNA-200b suppresses the invasion and migration of hepatocellular carcinoma by downregulating RhoA and circRNA_000839
title_short MicroRNA-200b suppresses the invasion and migration of hepatocellular carcinoma by downregulating RhoA and circRNA_000839
title_sort microrna 200b suppresses the invasion and migration of hepatocellular carcinoma by downregulating rhoa and circrna 000839
url https://doi.org/10.1177/1010428317719577
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AT yongfengliu microrna200bsuppressestheinvasionandmigrationofhepatocellularcarcinomabydownregulatingrhoaandcircrna000839
AT qianxu microrna200bsuppressestheinvasionandmigrationofhepatocellularcarcinomabydownregulatingrhoaandcircrna000839